{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:17:53Z","timestamp":1787807873038,"version":"build-2784847793"},"reference-count":29,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2018,12]]},"abstract":"\n Background:<\/jats:italic> The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials.<\/jats:p>\n \n Methods:<\/jats:italic> Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised Staphylococcus<\/jats:italic> spp., such as S. aureus<\/jats:italic>, Micrococcus<\/jats:italic> spp., Enterococcus<\/jats:italic> spp., including E. faecium<\/jats:italic>, E. faecalis<\/jats:italic>, and E. gallinarum<\/jats:italic>, Streptococcus<\/jats:italic> spp., like S. pyogenes<\/jats:italic>, S. agalactiae<\/jats:italic>, and S. pneumoniae<\/jats:italic>, Enterobacteriaceae<\/jats:italic>, such as Escherichia coli<\/jats:italic>, Klebsiella pneumoniae<\/jats:italic> and Salmonella<\/jats:italic> spp., Pseudomonas aeruginosa<\/jats:italic>, Stenotrophomonas maltophilia<\/jats:italic>, and Bacteroides<\/jats:italic> spp.<\/jats:p>\n \n Results:<\/jats:italic> A total of 955 blood culture materials were assessed with FISH. In 21 (2.2%) instances, FISH reaction led to non-interpretable results. With few exemptions, the tested FISH probes showed acceptable test characteristics even in the routine setting, with a sensitivity ranging from 28.6% (Bacteroides<\/jats:italic> spp.) to 100% (6 probes) and a specificity of >95% in all instances.<\/jats:p>\n \n Conclusion:<\/jats:italic> If sophisticated rapid diagnostic methods like mass spectrometry from blood culture materials are not available, FISH provides an option for rapid differentiation for laboratories in resource-limited settings.<\/jats:p>","DOI":"10.1556\/1886.2018.00024","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T11:30:33Z","timestamp":1544614233000},"page":"135-141","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of FISH for blood cultures under diagnostic real-life conditions"],"prefix":"10.1556","volume":"8","author":[{"given":"Annalena","family":"Reitz","sequence":"first","affiliation":[{"name":"University Hospital of the Goethe University, Frankfurt\/Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Poppert","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"Faculty of Medicine, University Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Rieker","sequence":"additional","affiliation":[{"name":"MVZ Humangenetik Ulm, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany"},{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Hospital Rostock, Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref1","series-title":"Crit Rev Microbiol","first-page":"263","article-title":"Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review","volume":"43","author":"Frickmann\n H","year":"2017","unstructured":"Frickmann\n H\n , ZautnerAE, MoterA, KikhneyJ, HagenRM, StenderH, et al\n Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review. Crit Rev Microbiol. 2017;43:263\u201393.28129707"},{"key":"ref2","series-title":"J Clin Microbiol.","first-page":"830","article-title":"Fluorescent in situ hybridisation allows rapid identification of microorganisms in blood cultures","volume":"38","author":"Kempf\n VAJ","year":"2000","unstructured":"Kempf\n VAJ\n , TrebesiusK, AutenriethIB. Fluorescent in situ hybridisation allows rapid identification of microorganisms in blood cultures. J Clin Microbiol.2000;38:830\u20138."},{"key":"ref3","series-title":"Med Dosw Mikrobiol","first-page":"319","article-title":"Comparision of the sensitivity of detection of bacteria in human blood using classic culture methods and molecular techniques: PCR and FISH","volume":"57","author":"Gosiewski\n T","year":"2005","unstructured":"Gosiewski\n T\n , KasprzykA, StrusM. Comparision of the sensitivity of detection of bacteria in human blood using classic culture methods and molecular techniques: PCR and FISH. Med Dosw Mikrobiol. 2005;57:319\u201325.16494209"},{"key":"ref4","series-title":"J Clin Microbiol","first-page":"3424","article-title":"Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization","volume":"45","author":"Wellinghausen\n N","year":"2007","unstructured":"Wellinghausen\n N\n , BartelM, EssigA, PoppertS. Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization. J Clin Microbiol. 2007;45:3424\u20136.17670922"},{"key":"ref5","series-title":"J Med Microbiol","first-page":"65","article-title":"Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure","volume":"59","author":"Poppert\n S","year":"2010","unstructured":"Poppert\n S\n , RieckerM, WellinghausenN, FrickmannH, EssigA. Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure. J Med Microbiol. 2010;59:65\u20138.19797464"},{"key":"ref6","series-title":"Eu J Microbiol Imunol (Bd)","first-page":"289","article-title":"Rapid identification of Acinetobacter spp. by fluorescence in situ hybridization (FISH) from colony and blood culture material","volume":"1","author":"Frickmann\n H","year":"2011","unstructured":"Frickmann\n H\n , EssigA, HagenRM, RieckerM, JerkeK, EllisonD, et al\n Rapid identification of Acinetobacter spp. by fluorescence in situ hybridization (FISH) from colony and blood culture material. Eu J Microbiol Imunol (Bd). 2011;1:289\u201396."},{"key":"ref7","series-title":"In J Med Microbiol","first-page":"277","article-title":"Fluorescence in situ hybridization (FISH) for rapid identification of Salmonella spp. from agar and blood culture broth \u2013 An option for the tropics?","volume":"303","author":"Frickmann\n H","year":"2013","unstructured":"Frickmann\n H\n , H\u00e4nleA, EssigA, DekkerD, BoahenK, AcquahS, et al\n Fluorescence in situ hybridization (FISH) for rapid identification of Salmonella spp. from agar and blood culture broth \u2013 An option for the tropics?\n In J Med Microbiol. 2013;303:277\u201384."},{"key":"ref8","series-title":"Clin Infect Dis","first-page":"1101","article-title":"Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study","volume":"56","author":"Clerc\n O","year":"2013","unstructured":"Clerc\n O\n , Prod'homG, VogneC, BizziniA, CalandraT, GreubG. Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study. Clin Infect Dis. 2013;56:1101\u20137.23264363"},{"key":"ref9","series-title":"Eur J Clin Microbiol Infect Dis","first-page":"699","article-title":"A differential centrifugation protocol and validation criterion for enhancing mass spectrometry (MALDI-TOF) results in microbial identification using blood culture growth bottles","volume":"32","author":"March-Rossell\u00f3\n GA","year":"2013","unstructured":"March-Rossell\u00f3\n GA\n , Mu\u00f1oz-MorenoMF, Garc\u00eda-Loygorri-Jord\u00e1n de Urri\u00e9sMC, Bratos-P\u00e9rezMA. A differential centrifugation protocol and validation criterion for enhancing mass spectrometry (MALDI-TOF) results in microbial identification using blood culture growth bottles. Eur J Clin Microbiol Infect Dis. 2013;32:699\u2013704.23274860"},{"key":"ref10","series-title":"J Clin Microbiol","first-page":"1947","article-title":"Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes","volume":"43","author":"S\u00f8gaard\n M","year":"2005","unstructured":"S\u00f8gaard\n M\n , StenderH, Sch\u00f8nheyderHC. Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes. J Clin Microbiol. 2005;43:1947\u20139."},{"key":"ref11","series-title":"J Med Microbiol","first-page":"914","article-title":"Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures","volume":"56","author":"S\u00f8gaard\n M","year":"2007","unstructured":"S\u00f8gaard\n M\n , HansenDS, FiandacaMJ, StenderH, Sch\u00f8nheyderHC. Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures. J Med Microbiol. 2007;56:914\u20137.17577055"},{"key":"ref12","series-title":"J Clin Microbiol","first-page":"830","article-title":"Utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa","volume":"47","author":"Peleg\n AY","year":"2009","unstructured":"Peleg\n AY\n , TilahunY, FiandacaMJ, D\u2019AgataEMC, VenkataramanL, Moellering Jr. RC, et alUtility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa. J Clin Microbiol. 2009;47:830\u20132.19116347"},{"key":"ref13","series-title":"J Clin Microbiol","first-page":"2268","article-title":"Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures","volume":"48","author":"Morgan\n M","year":"2010","unstructured":"Morgan\n M\n , MarloweE, Della-LattaP, SalimniaH, Novak-WeekleyS, WuF, et al\n Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol. 2010;48:2268\u201370.20357212"},{"key":"ref14","series-title":"J Clin Microbiol","first-page":"2259","article-title":"Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay","volume":"49","author":"Della-Latta\n P","year":"2011","unstructured":"Della-Latta\n P\n , SalimniaH, PainterT, WuF, ProcopGW, WilsonDA, et al\n Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay. J Clin Microbiol. 2011;49:2259\u201361.21490185"},{"key":"ref15","series-title":"J Mol Diagn","first-page":"835","article-title":"Rapid Identification of Pathogens with the hemoFISH Test Applying a Novel Beacon-Based Fluorescence in Situ Hybridization (bbFISH) Technology in Positive Blood Culture Bottles","volume":"15","author":"Leitner\n E","year":"2013","unstructured":"Leitner\n E\n , ScherrS, StrempflC, KrauseR, FeierlG, GrisoldAJ. Rapid Identification of Pathogens with the hemoFISH Test Applying a Novel Beacon-Based Fluorescence in Situ Hybridization (bbFISH) Technology in Positive Blood Culture Bottles. J Mol Diagn. 2013;15:835\u20139.23988621"},{"key":"ref16","series-title":"Diagn Microbiol Infect Dis","first-page":"214","article-title":"Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization","volume":"66","author":"Poppert\n S","year":"2010","unstructured":"Poppert\n S\n , RieckerM, EssigA. Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization. Diagn Microbiol Infect Dis. 2010;66:214\u20136.19815363"},{"key":"ref17","series-title":"J Clin Microbiol","first-page":"119","article-title":"Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice","volume":"44","author":"Peters\n RP","year":"2006","unstructured":"Peters\n RP\n , SavelkoulPH, Simoons-SmitAM, DannerSA, Vandenbroucke-GraulsCM, van AgtmaelMA. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol. 2006;44:119\u201323.16390958"},{"key":"ref18","series-title":"Indian J Med Microbiol","first-page":"391","article-title":"Rapid detection of non-Enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization","volume":"25","author":"Wong\n EH","year":"2007","unstructured":"Wong\n EH\n , SubramaniamG, NavaratnamP, SekaranSD. Rapid detection of non-Enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization. Indian J Med Microbiol. 2007;25:391\u20134.18087092"},{"key":"ref19","series-title":"Int J Antimicrob Agents","first-page":"S51","article-title":"Fluorescence in situ hybridisation (FISH) accelerates identification of Gram-positive cocci in positive blood cultures","volume":"32","author":"Gescher\n DM","year":"2008","unstructured":"Gescher\n DM\n , KovacevicD, SchmiedelD, SiemoneitS, MallmannC, HalleE, et al\n Fluorescence in situ hybridisation (FISH) accelerates identification of Gram-positive cocci in positive blood cultures. Int J Antimicrob Agents. 2008;32:S51\u20139.18718741"},{"key":"ref20","series-title":"Acta Microbiol Immunol Hung","first-page":"225","article-title":"Rapid identification of pathogens in blood culture with fluorescent in situ hybridization (FISH)","volume":"57","author":"Horv\u00e1th\n A","year":"2010","unstructured":"Horv\u00e1th\n A\n , Krist\u00f3fK, Konkoly-ThegeM, NagyK. Rapid identification of pathogens in blood culture with fluorescent in situ hybridization (FISH). Acta Microbiol Immunol Hung. 2010;57:225\u201334.20870594"},{"key":"ref21","series-title":"Open Microbiol J","first-page":"123","article-title":"Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis","volume":"4","author":"S\u00f6nksen\n UW","year":"2010","unstructured":"S\u00f6nksen\n UW\n , ChristensenJJ, NielsenL, HesselbjergA, HansenDS, BruunB. Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis. Open Microbiol J. 2010;4:123\u201331.21347215"},{"key":"ref22","series-title":"J Microbiol Methods","first-page":"209","article-title":"A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level","volume":"92","author":"Kierzkowska\n M","year":"2013","unstructured":"Kierzkowska\n M\n , MajewskaA, KuthanRT, Sawicka-GrzelakA, M\u0142ynarczykG. A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level. J Microbiol Methods. 2013;92:209\u201312."},{"key":"ref23","series-title":"J Clin Microbiol","first-page":"131","article-title":"Comparison of the Staphylococcus QuickFISH BC test with the tube coagulase test performed on positive blood cultures for evaluation and application in a clinical routine setting","volume":"51","author":"Carretto\n E","year":"2013","unstructured":"Carretto\n E\n , BardaroM, RusselloG, MirraM, ZuelliC, BarbariniD. Comparison of the Staphylococcus QuickFISH BC test with the tube coagulase test performed on positive blood cultures for evaluation and application in a clinical routine setting. J Clin Microbiol. 2013;51:131\u20135."},{"key":"ref24","series-title":"Mycoses","first-page":"e114","article-title":"Evaluation of fluorescence in situ hybridisation (FISH) for the identification of Candida albicans in comparison with three phenotypic methods","volume":"55","author":"Lakner\n A","year":"2012","unstructured":"Lakner\n A\n , EssigA, FrickmannH, PoppertS. Evaluation of fluorescence in situ hybridisation (FISH) for the identification of Candida albicans in comparison with three phenotypic methods. Mycoses. 2012;55:e114\u201323."},{"key":"ref25","series-title":"Mycoses","first-page":"521","article-title":"Rapid identification of yeast by fluorescence in situ hybridisation from broth and blood cultures","volume":"55","author":"Frickmann\n H","year":"2012","unstructured":"Frickmann\n H\n , LaknerA, EssigA, PoppertS. Rapid identification of yeast by fluorescence in situ hybridisation from broth and blood cultures. Mycoses. 2012;55:521\u201331.22681159"},{"key":"ref26","series-title":"J Clinical Microbiol","first-page":"818","article-title":"Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients","volume":"38","author":"Hogardt\n M","year":"2000","unstructured":"Hogardt\n M\n , TrebesiusK, GeigerAM, HornefM, RoseneckerJ, HeesemannJ. Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J Clinical Microbiol. 2000;38:818\u201325."},{"key":"ref27","series-title":"J Bacteriol","first-page":"762","article-title":"Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology","volume":"172","author":"Amann\n RI","year":"1990","unstructured":"Amann\n RI\n , KrumholzL, StahlDA. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol. 1990;172:762\u201370.1688842"},{"key":"ref28","series-title":"Med Microbiol Immunol","first-page":"169","article-title":"Culture independent and rapid identification of bacterial pathogens in necrotizing fasciitis and streptococcal toxic shock syndrome by fluorescence in situ hybridization","volume":"188","author":"Trebesius\n K","year":"2000","unstructured":"Trebesius\n K\n , LeitritzL, AdlerK, SchubertS, AutenriethIB, HeesemannJ. Culture independent and rapid identification of bacterial pathogens in necrotizing fasciitis and streptococcal toxic shock syndrome by fluorescence in situ hybridization. Med Microbiol Immunol. 2000;188:169\u201375.10917153"},{"key":"ref29","series-title":"Appl Environ Microbiol","first-page":"3336","article-title":"Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes","volume":"64","author":"Franks\n AH","year":"1998","unstructured":"Franks\n AH\n , HarmsenHJ, RaangsGC, JansenGJ, SchutF, WellingGW. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol. 1998;64:3336\u201345.9726880"}],"container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/1886.2018.00024","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/4\/article-p135.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/4\/article-p135.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/4\/article-p135.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:37:14Z","timestamp":1704299834000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/4\/article-p135.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":29,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/1886.2018.00024","relation":{},"ISSN":["2062-8633"],"issn-type":[{"value":"2062-8633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12]]}}}