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In endemic regions, infected dogs are the main parasitic reservoir host of human Visceral leishmaniasis (VL) infection. Vaccination of dogs against <jats:italic>Leishmania<\/jats:italic> infection constitutes an important strategy to prevent or to better control CVL, thus, a serological test that can discriminate between antibodies induced by immunization versus infection is highly desirable in order to improve and simplify diagnosis. Here, four recombinant proteins were evaluated for their ability to detect and differentiate between dogs that are infected with <jats:italic>Leishmania<\/jats:italic> or have been immunized with the anti-<jats:italic>Leishmania<\/jats:italic> vaccine Leish-Tec\u00ae. Receiver operating characteristic (ROC) curve analysis of the four <jats:italic>Leishmania<\/jats:italic>-specific IgG ELISA revealed superior performance of rK28, followed by rKLO8, rK39 and rLb6H. The rK28-based ELISA revealed not only the best accuracy against CVL, but also the lowest cross-reactivity with sera from Leish-Tec\u00ae immunized dogs. Our data show that the rK28-based ELISA is highly suitable for CVL screening as it shows high sensitivity with simultaneous low cross-reactivity. Further, the high specificity of the rKLO8 indicates its suitability for the confirmation of CVL diagnosis.<\/jats:p>","DOI":"10.1556\/1886.2020.00018","type":"journal-article","created":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T03:32:55Z","timestamp":1598671975000},"page":"165-171","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":11,"title":["Performance of recombinant proteins in diagnosis and differentiation of canine visceral leishmaniasis infected and vaccinated dogs"],"prefix":"10.1556","volume":"10","author":[{"given":"Ingrid E.","family":"Pereira","sequence":"first","affiliation":[{"name":"1Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyssia P.","family":"Silva","sequence":"additional","affiliation":[{"name":"1Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura M.","family":"Menegati","sequence":"additional","affiliation":[{"name":"1Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aimara C.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"2Zoonosis Control Center, Municipal Health Secretariat, 35022-280, Governador Valadares, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine A. O.","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"2Zoonosis Control Center, Municipal Health Secretariat, 35022-280, Governador Valadares, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria De Lourdes P.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"3Santo Agostinho Veterinary Hospital, 30180-003, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elfadil","family":"Abass","sequence":"additional","affiliation":[{"name":"4Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm S.","family":"Duthie","sequence":"additional","affiliation":[{"name":"5HDT Biotech, 98102, Seattle, United 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