{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:07:22Z","timestamp":1790258842859,"version":"4.1.0"},"reference-count":65,"publisher":"Akademiai Kiado Zrt.","issue":"3","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["www.akjournals.com"],"crossmark-restriction":true},"short-container-title":["EUJMI"],"published-print":{"date-parts":[[2026,9,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The investigation was conducted to evaluate a robust commercial multiplexed species- or species complex-specific real-time PCR assay for human malaria applicable with mobile cyclers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A well-characterized sample set of blood samples from travelers with high pre-test probability of being infected with plasmodial species was assessed with the\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      qPCR panel (Bioeksen, Istanbul, Turkey) against a composite reference standard comprising microscopy following the protocols by World Health Organization in combination with four nucleic acid amplification tests for malaria.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      From 1,020 included samples, composite reference standard testing identified 248\u00a0(24.3%) as positive for plasmodial DNA, comprising 217x\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      , 14x\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      , 8x\n                      <jats:italic>Plasmodium ovale<\/jats:italic>\n                      complex, 6x\n                      <jats:italic>Plasmodium malariae<\/jats:italic>\n                      and 3 samples containing DNA of both\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      and\n                      <jats:italic>P. vivax<\/jats:italic>\n                      . Diagnostic accuracy of the evaluated PCR in terms of sensitivity and specificity was 96.8% (240\/248) and 99.5% (768\/772). The resulting positive and negative predictive values were 98.4% (240\/244) and 99.0% (768\/776). False-negative results were associated with either submicroscopic parasitemia or parasitemia at the threshold of microscopic detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The assessed multiplex real-time PCR assay showed similar performance characteristics like comparable diagnostic assays and thus proved to be suitable for the differentiation of plasmodia in the non-endemic setting.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1556\/1886.2026.00033","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T07:43:39Z","timestamp":1782891819000},"page":"217-230","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Head-to-head evaluation of a commercial real-time PCR assay targeting plasmodial species\u00a0causing human malaria against a\u00a0composite reference standard"],"prefix":"10.1556","volume":"16","author":[{"given":"Marc Tim","family":"Hennies","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"May","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Tanida","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egbert","family":"Tannich","sequence":"additional","affiliation":[{"name":"National Reference Centre for Tropical Pathogens, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8967-9528","authenticated-orcid":true,"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany"},{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"B1","series-title":"WHO guidelines for malaria, 30 November 2024","doi-asserted-by":"publisher","DOI":"10.2471\/B09146","year":"2024","unstructured":"WHO guidelines for malaria, 30 November 2024. 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