{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T11:10:24Z","timestamp":1710846624486},"reference-count":30,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiol Hung"],"published-print":{"date-parts":[[2017,6,21]]},"abstract":"Atherosclerosis is a disease caused by a build-up of fatty plaques and cholesterol in the arteries. The lumen of the vessels is obliterated resulting in restricted blood supply to tissues. In ischemic conditions, the cytosolic Ca2+<\/jats:sup> level of skeletal muscle may increase, indicating the alteration of Ca2+<\/jats:sup> removal mechanisms. Ca2+<\/jats:sup> is transported from cytosol into the sarcoplasmic reticulum by Ca2+<\/jats:sup> ATPase (SERCA), with its 1a isoform expressed in adult, while its 1b isoform in neonatal and regenerating fast-twitch skeletal muscle. To investigate the role of these isoforms in ischemic skeletal muscle, biopsies from musculus biceps femoris<\/jats:italic> of patients who underwent amputation due to atherosclerosis were examined. Samples were removed from the visibly healthy and hypoxia-affected tissue. Significantly increased SERCA1a expression was detected under the ischemic conditions (246\u00a0\u00b1\u00a069%; p<\/jats:italic>\u00a0<\u00a00.05) compared with the healthy tissue. Furthermore, the ratio of SERCA1a-positive fibers was slightly increased (46\u00a0\u00b1\u00a04% in healthy tissue and 60\u00a0\u00b1\u00a05% in ischemic tissue; p<\/jats:italic>\u00a0>\u00a00.05), whereas SERCA2a did not change. In addition, in primary cultures derived from hypoxia-affected tissue, the diameter and fusion index of myotubes were significantly increased (30\u00a0\u00b1\u00a01.6\u00a0\u00b5m vs. 41\u00a0\u00b1\u00a02.4\u00a0\u00b5m and 31\u00a0\u00b1\u00a04% vs. 45\u00a0\u00b1\u00a03%; p<\/jats:italic>\u00a0<\u00a00.05). We propose that the increased SERCA1a expression indicates the existence and location of compensating mechanisms in ischemic muscle.<\/jats:p>","DOI":"10.1556\/2060.104.2017.2.1","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T07:30:51Z","timestamp":1498462251000},"page":"183-192","source":"Crossref","is-referenced-by-count":3,"title":["Alteration of sarcoplasmic reticulum Ca2+ ATPase expression in lower limb ischemia caused by atherosclerosis obliterans"],"prefix":"10.1556","volume":"104","author":[{"given":"J","family":"Fodor","sequence":"first","affiliation":[{"name":"1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Gomba-T\u00f3th","sequence":"additional","affiliation":[{"name":"1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Ol\u00e1h","sequence":"additional","affiliation":[{"name":"1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Z\u00e1dor","sequence":"additional","affiliation":[{"name":"2 Department of Biochemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zs Cs","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"3 Limb Surgery Ward, Ken\u00e9zy Hospital and Outpatient Clinic, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Ioannis","sequence":"additional","affiliation":[{"name":"3 Limb Surgery Ward, Ken\u00e9zy Hospital and Outpatient Clinic, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"3 Limb Surgery Ward, Ken\u00e9zy Hospital and Outpatient Clinic, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"4 Pathology Department, Ken\u00e9zy Hospital and Outpatient Clinic, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Csernoch","sequence":"additional","affiliation":[{"name":"1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref281","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1007\/s00424-014-1671-3","article-title":"The neonatal sarcoplasmic\/endoplasmic reticulum Ca2+ ATPase (SERCA1b): a neglected pump in scope","volume":"467","author":"Z\u00e1dor","year":"2015","journal-title":"Pflugers Arch."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s10974-015-9411-7","article-title":"HMGB1 expression and muscle regeneration in idiopathic inflammatory myopathies and degenerative joint diseases","volume":"36","author":"Cseri","year":"2015","journal-title":"J. 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