{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T18:15:02Z","timestamp":1649096102175},"reference-count":46,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiol Hung"],"published-print":{"date-parts":[[2018,3,30]]},"abstract":"Moderate hypothermia (25\u201331\u00a0\u00b0C) may have a significant influence on vascular tone. At present, very little is known about the role of endothelial nitric oxide on the hypothermia-induced responses. In this study, we investigated the effect of hypothermia (to 28\u00a0\u00b0C) on the vasodilatation induced by verapamil, a phenylalkylamine calcium channel blocker (10\u22129<\/jats:sup>\u20133\u2009\u00d7\u200910\u22124<\/jats:sup>\u00a0M) and dihydropyridines, amlodipine (10\u22129<\/jats:sup>\u20133\u2009\u00d7\u200910\u22124<\/jats:sup>\u00a0M), and benidipine (10\u22129<\/jats:sup>\u201310\u22123<\/jats:sup>\u00a0M) on 5-hydroxytryptamine (5-HT or serotonin) precontracted calf cardiac veins. Furthermore, the role of nitric oxide in the hypothermia-induced responses was analyzed. Ring preparations of veins obtained from calf hearts were suspended in organ baths containing 15\u00a0ml of Krebs\u2013Henseleit solution, maintained at 37\u00a0\u00b0C, and continuously gassed with 95% O2<\/jats:sub>\u20135% CO2<\/jats:sub>. After a resting period, verapamil, amlodipine, and benidipine were applied cumulatively on serotonin (10\u22126<\/jats:sup>\u00a0M) precontracted calf cardiac vein rings and induced concentration-dependent relaxations. In another part of the study, the medium temperature was decreased to 28\u00a0\u00b0C after the preparations were contracted with 5-HT, then cumulative concentrations of verapamil, amlodipine, or benidipine were added. During hypothermia, the pIC50<\/jats:sub> value, but not the maximal response, to all blockers were significantly higher than at 37\u00a0\u00b0C. Hypothermia in the presence of NG<\/jats:sup>-nitro-l<\/jats:sc>-arginine methyl ester (L-NAME, 10\u22124<\/jats:sup>\u00a0M) decreased the pIC50<\/jats:sub> and E<\/jats:italic>max<\/jats:sub> values to verapamil, amlodipine, and benidipine. Only one blocker was tested in each preparation. These results suggest that nitric oxide may play a role in the hypothermia-induced changes in vasodilation caused by verapamil, amlodipine, and benidipine in calf cardiac vein, but further research is needed to explain the complete mechanism.<\/jats:p>","DOI":"10.1556\/2060.105.2018.1.2","type":"journal-article","created":{"date-parts":[[2018,3,31]],"date-time":"2018-03-31T04:40:13Z","timestamp":1522471213000},"page":"53-60","source":"Crossref","is-referenced-by-count":0,"title":["Moderate hypothermia and responses to calcium channel blockers \u2013 Role of the nitric oxide"],"prefix":"10.1556","volume":"105","author":[{"given":"S","family":"Canbolat","sequence":"first","affiliation":[{"name":"1 Meram Faculty of Medicine, Department of Medical Pharmacology, Necmettin Erbakan University, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KE","family":"Nurullahoglu Atalik","sequence":"additional","affiliation":[{"name":"1 Meram Faculty of Medicine, Department of Medical Pharmacology, Necmettin Erbakan University, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref91","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1748-1716.1994.tb09766.x","article-title":"Cooling effects on the histaminergic response of rabbit ear and femoral arteries: role of the endothelium","volume":"151","author":"Fernandez","year":"2007","journal-title":"Acta Physiol. 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Listy."},{"key":"ref391","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1556\/2060.103.2016.4.2","article-title":"Moderate hypothermia attenuates \u03b11-adrenoceptor-mediated contraction in human varicose spermatic vein: the role of nitric oxide (short communication)","volume":"103","author":"Nurullahoglu-Atalik","year":"2016","journal-title":"Physiol. Int."},{"key":"ref261","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1016\/j.bcp.2012.02.025","article-title":"Dehydroepiandrosterone (DHEA) inhibits voltage-gated T-type calcium channels","volume":"83","author":"Chevalier","year":"2012","journal-title":"Biochem. Pharmacol."},{"key":"ref221","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.ejphar.2007.04.057","article-title":"Cooling augments vasoconstriction mediated by 5-HT1 and alpha2-adrenoceptors in the isolated equine digital vein: involvement of Rho kinase","volume":"569","author":"Zerpa","year":"2007","journal-title":"Eur. J. 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