{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:15:47Z","timestamp":1787022947664,"version":"build-2736575974"},"reference-count":44,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiol Hung"],"published-print":{"date-parts":[[2018,3,30]]},"abstract":"<jats:sec sec-type=\"purpose\">\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Acute lower extremity ischemia is still a main cause of mortality and morbidity in orthopedic traumatology and reconstructive surgery. In acute lower extremity ischemia, the skeletal muscles are the tissues that are the most vulnerable to ischemia. The aim of this study was to evaluate the effects of iloprost (IL) therapy on skeletal muscle contractile impairment and mitochondrial degeneration in an acute lower extremity ischemia-reperfusion rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"main methods\">\n                    <jats:title>Main Methods<\/jats:title>\n                    <jats:p>Forty Wistar albino rats were randomly divided into a control group and four experimental groups. Experimental groups were either subjected to 2\u00a0h of lower extremity ischemia followed by a 4-h reperfusion period or to 4\u00a0h of ischemia followed by an 8-h reperfusion period. Except for the animals in the control group, all animals received IL (1\u00a0ng\/kg\/min) or saline (1\u00a0ml\/kg) by intraperitoneal infusion for 10\u00a0min immediately before reperfusion. At the end of the recording of skeletal muscle electrical activity and contractility, all rats were sacrificed by decapitation and muscle samples of lower extremity were immediately harvested for histopathologic analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"results\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>After ischemia-reperfusion, a breakdown in the force\u2013frequency curves of extensor digitorum longus muscle was observed, showing the diminished muscle contractility. However, IL significantly improved muscle contractility following injury induced by 2\u00a0h of ischemia followed by a 4-h reperfusion period. In addition, IL partially ameliorated mitochondrial degeneration in the muscle cells of ischemia groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"conclusion\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study indicates that immediate IL therapy repairs muscle damage especially after 2\u00a0h of ischemia and 4\u00a0h of reperfusion and therefore that IL improves contractile function.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1556\/2060.105.2018.1.5","type":"journal-article","created":{"date-parts":[[2018,3,31]],"date-time":"2018-03-31T00:40:13Z","timestamp":1522456813000},"page":"61-75","source":"Crossref","is-referenced-by-count":7,"title":["Effect of iloprost on contractile impairment and mitochondrial degeneration in ischemia-reperfusion of skeletal muscle"],"prefix":"10.1556","volume":"105","author":[{"given":"Z","family":"Bagis","sequence":"first","affiliation":[{"name":"1 Faculty of Medicine, Department of Cardiovascular Surgery, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Ozeren","sequence":"additional","affiliation":[{"name":"1 Faculty of Medicine, Department of Cardiovascular Surgery, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Buyukakilli","sequence":"additional","affiliation":[{"name":"2 Faculty of Medicine, Department of Biophysics, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Balli","sequence":"additional","affiliation":[{"name":"3 Medical Faculty, Department of Histology and Embryology, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Yaman","sequence":"additional","affiliation":[{"name":"4 Department of Biophysics, Kahramanmaras S\u00fct\u00e7\u00fc Imam University, Kahramanmaras, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Yetkin","sequence":"additional","affiliation":[{"name":"3 Medical Faculty, Department of Histology and Embryology, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Ovla","sequence":"additional","affiliation":[{"name":"5 Faculty of Medicine, Department of Biostatistics, Mersin University, Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref341","first-page":"182","article-title":"Iloprost and pentoxifylline attenuate ischemia-reperfusion injury in skeletal muscle in rabbit model","volume":"14","author":"Emrecan","year":"2008","journal-title":"Ulus. 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Res."},{"key":"ref181","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.bjan.2016.09.004","article-title":"Effect of dexmedetomidine on acute lung injury in experimental ischemia-reperfusion model","volume":"67","author":"K\u00fc\u00e7\u00fckebe","year":"2017","journal-title":"Braz. J. Anesthesiol."},{"key":"ref141","volume-title":"Guyton and Hall Textbook of Medical Physiology","author":"Hall","year":"2015"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/0002-8703(92)90644-B","article-title":"Reduction of reperfusion injury with preoperative rapid intravenous infusion of taurine during myocardial revascularization","volume":"123","author":"Milei","year":"1992","journal-title":"Am. Heart J."},{"key":"ref201","doi-asserted-by":"crossref","first-page":"453748","DOI":"10.1155\/2015\/453748","article-title":"The effect of iloprost and N-acetylcysteine on skeletal muscle injury in an acute aortic ischemia-reperfusion model: an experimental study","volume":"2015","author":"Tiryakioglu","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref451","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1097\/BOT.0b013e31815b3591","article-title":"The combined influence of hemorrhage and tourniquet application on the recovery of muscle function in rats","volume":"22","author":"Walters","year":"2008","journal-title":"J. Orthop. Trauma"},{"key":"ref251","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1067\/mva.1990.25129","article-title":"Iloprost attenuates the increased permeability in skeletal muscle after ischemia and reperfusion","volume":"12","author":"Blebea","year":"1990","journal-title":"J. Vasc. 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Antropol."},{"key":"ref191","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/BOT.0b013e31823dea55","article-title":"The effects of hypothermia and L-arginine on skeletal muscle function in ischemia-reperfusion injury","volume":"26","author":"Stahl","year":"2012","journal-title":"J. Orthop. Trauma"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/0022-4804(87)90060-6","article-title":"Effect of superoxide dismutase plus catalase on Ca2+ transport in ischemic and reperfused skeletal muscle","volume":"42","author":"Lee","year":"1987","journal-title":"J. Surg. Res."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/S0967-2109(02)00070-4","article-title":"The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review","volume":"10","author":"Blaisdell","year":"2002","journal-title":"Cardiovasc. 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Endothelium Lymphatics"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1085\/jgp.98.5.1043","article-title":"In vivo magnetic and electric recordings from nerve bundles and single motor units in mammalian skeletal muscle. Correlations with muscle force","volume":"98","author":"Gielen","year":"1991","journal-title":"J. Gen. Physiol."},{"key":"ref421","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/BOT.0b013e31823dea55","article-title":"The effects of hypothermia and L-arginine on skeletal muscle function in ischemia-reperfusion injury","volume":"26","author":"Stahl","year":"2012","journal-title":"J. Orthop. Trauma"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/S0967-2109(02)00070-4","article-title":"The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review","volume":"10","author":"Blaisdell","year":"2002","journal-title":"Cardiovasc. Surg."},{"key":"ref281","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.lfs.2012.03.036","article-title":"The effect of tumor necrosis factor-alfa inhibitor soon after hypoxia-ischemia on heart in neonatal rats","volume":"90","author":"B\u00fcy\u00fckakilli","year":"2012","journal-title":"Life Sci."},{"key":"ref101","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.orthres.2003.10.015","article-title":"Ischemic-preconditioning does not prevent neuromuscular dysfunction after ischemia-reperfusion injury","volume":"22","author":"Eastlack","year":"2004","journal-title":"J. Orthop. Res."},{"key":"ref231","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jss.2013.09.031","article-title":"The effects of iloprost on ischemia-reperfusion injury in skeletal muscles in a rodent model","volume":"187","author":"Avci","year":"2014","journal-title":"J. Surg. Res."},{"key":"ref221","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1097\/BOT.0b013e31815b3591","article-title":"The combined influence of hemorrhage and tourniquet application on the recovery of muscle function in rats","volume":"22","author":"Walters","year":"2008","journal-title":"J. Orthop. Trauma"},{"key":"ref431","doi-asserted-by":"crossref","first-page":"453748","DOI":"10.1155\/2015\/453748","article-title":"The effect of iloprost and N-acetylcysteine on skeletal muscle injury in an acute aortic ischemia-reperfusion model: an experimental study","volume":"2015","author":"Tiryakioglu","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref301","doi-asserted-by":"crossref","first-page":"e65","DOI":"10.1016\/j.jss.2011.05.061","article-title":"Treatment of tourniquet-induced ischemia reperfusion injury with muscle progenitor cells","volume":"170","author":"Chen","year":"2011","journal-title":"J. Surg. Res."},{"key":"ref81","first-page":"171","article-title":"The effect of ischemia-reperfusion derived oxygen free radicals on skeletal muscle calcium metabolism","volume":"5","author":"Cronenwett","year":"1989","journal-title":"Microcirc. Endothelium Lymphatics"},{"key":"ref111","first-page":"182","article-title":"Iloprost and pentoxifylline attenuate ischemia-reperfusion injury in skeletal muscle in rabbit model","volume":"14","author":"Emrecan","year":"2008","journal-title":"Ulus. Travma. Acil Cerrahi Derg."},{"key":"ref391","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/0002-8703(92)90644-B","article-title":"Reduction of reperfusion injury with preoperative rapid intravenous infusion of taurine during myocardial revascularization","volume":"123","author":"Milei","year":"1992","journal-title":"Am. Heart J."},{"key":"ref271","first-page":"693","article-title":"Alpha-tocopherol (vitamin E) and iloprost attenuate reperfusion injury in skeletal muscle ischemia\/reperfusion injury","volume":"43","author":"Bozkurt","year":"2002","journal-title":"J. Cardiovasc. Surg."},{"key":"ref151","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/0022-4804(87)90060-6","article-title":"Effect of superoxide dismutase plus catalase on Ca2+ transport in ischemic and reperfused skeletal muscle","volume":"42","author":"Lee","year":"1987","journal-title":"J. Surg. Res."},{"key":"ref261","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1476-9255-1-2","article-title":"The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles","volume":"1","author":"Bortolotto","year":"2004","journal-title":"J. Inflamm. (Lond.)"},{"key":"ref291","first-page":"25","article-title":"The role of prostacyclin in modifying acute hepatotoxicity of acetaminophen in mice","volume":"33","author":"Cavar","year":"2009","journal-title":"Coll. Antropol."},{"key":"ref171","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ejphar.2004.10.014","article-title":"The role of nitric oxide on contractile impairment during endotoxemia in rat diaphragm muscle","volume":"505","author":"Sara","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"ref01","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jss.2013.09.031","article-title":"The effects of iloprost on ischemia-reperfusion injury in skeletal muscles in a rodent model","volume":"187","author":"Avci","year":"2014","journal-title":"J. Surg. Res."},{"key":"ref401","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ejphar.2004.10.014","article-title":"The role of nitric oxide on contractile impairment during endotoxemia in rat diaphragm muscle","volume":"505","author":"Sara","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"ref91","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1177\/1538574413496481","article-title":"L-Carnitine pretreatment protects slow-twitch skeletal muscles in a rat model of ischemia-reperfusion injury","volume":"47","author":"Demirel","year":"2013","journal-title":"Vasc. Endovascular Surg."},{"key":"ref41","first-page":"693","article-title":"Alpha-tocopherol (vitamin E) and iloprost attenuate reperfusion injury in skeletal muscle ischemia\/reperfusion injury","volume":"43","author":"Bozkurt","year":"2002","journal-title":"J. Cardiovasc. Surg."},{"key":"ref131","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1085\/jgp.98.5.1043","article-title":"In vivo magnetic and electric recordings from nerve bundles and single motor units in mammalian skeletal muscle. Correlations with muscle force","volume":"98","author":"Gielen","year":"1991","journal-title":"J. Gen. Physiol."}],"container-title":["Physiology International"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/2060.105.2018.1.5","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/2060\/105\/1\/article-p61.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akademiai.com\/doi\/full\/10.1556\/2060.105.2018.1.5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T21:32:30Z","timestamp":1644528750000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/doi\/10.1556\/2060.105.2018.1.5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":44,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/2060.105.2018.1.5","relation":{},"ISSN":["2498-602X"],"issn-type":[{"value":"2498-602X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,3]]}}}