{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T14:45:15Z","timestamp":1775227515369,"version":"3.50.1"},"reference-count":58,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol. Int."],"published-print":{"date-parts":[[2019,9,26]]},"abstract":"\n Objectives<\/jats:title>\n Impaired intestinal barrier function has been demonstrated in the pathophysiology of diarrhea-predominant irritable bowel syndrome (IBS-D). This study aimed to describe the intestinal ultrastructural findings in the intestinal mucosal layer of IBS-D patients.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n In total, 10 healthy controls and 10 IBS-D patients were analyzed in this study. The mucosa of each patient\u2019s rectosigmoid colon was first assessed by confocal laser endomicroscopy (CLE); next, biopsied specimens of these sites were obtained. Intestinal tissues of IBS-D patients and healthy volunteers were examined to observe cellular changes by transmission electron microscopy (TEM).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n CLE showed no visible epithelial damage or inflammatory changes in the colonic mucosa of IBS-D compared with healthy volunteers. On transmission electron microscopic examination, patients with IBS-D displayed a larger apical intercellular distance with a higher proportion of dilated (>20\u00a0nm) intercellular junctional complexes, which was indicative of impaired mucosal integrity. In addition, microvillus exfoliation, extracellular vesicle as well as increased presence of multivesicular bodies were visible in IBS-D patients. Single epithelial cells appeared necrotic, as characterized by cytoplasmic vacuolization, cytoplasmic swelling, and presence of autolysosome. A significant association between bowel habit, frequency of abdominal pain, and enlarged intercellular distance was found.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n This study showed ultrastructural alterations in the architecture of intestinal epithelial cells and intercellular junctional complexes in IBS-D patients, potentially representing a pathophysiological mechanism in IBS-D.<\/jats:p>\n <\/jats:sec>","DOI":"10.1556\/2060.106.2019.20","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T13:00:35Z","timestamp":1569589235000},"page":"225-235","source":"Crossref","is-referenced-by-count":11,"title":["Ultrastructure of intestinal mucosa in diarrhea-predominant irritable bowel syndrome"],"prefix":"10.1556","volume":"106","author":[{"given":"D-Y","family":"Zhao","sequence":"first","affiliation":[{"name":"1 Department of Gastroenterology, Puyang Oilfield General Hospital, Puyang, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q-Q","family":"Qi","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Long","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Li","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F-X","family":"Chen","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y-B","family":"Yu","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X-L","family":"Zuo","sequence":"additional","affiliation":[{"name":"2 Department of Gastroenterology, Qilu Hospital, Shandong University, Shandong Province, P. R. 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Hepatol."},{"key":"ref561","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1136\/gutjnl-2012-303857","article-title":"Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia","volume":"63","author":"Vanheel","year":"2014","journal-title":"Gut"},{"key":"ref471","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.bcmd.2005.04.001","article-title":"Phenotypic and functional characterization of intestinal epithelial exosomes","volume":"35","author":"Mallegol","year":"2005","journal-title":"Blood Cells Mol. Dis."},{"key":"ref551","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.gie.2012.11.006","article-title":"Breaks in the wall: increased gaps in the intestinal epithelium of irritable bowel syndrome patients identified by confocal laser endomicroscopy (with videos)","volume":"77","author":"Turcotte","year":"2013","journal-title":"Gastrointest. 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Lessons for and from the crypt","volume":"110","author":"Potten","year":"1990","journal-title":"Development"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1053\/j.gastro.2014.07.046","article-title":"Confocal endomicroscopy shows food-associated changes in the intestinal mucosa of patients with irritable bowel syndrome","volume":"147","author":"Fritscher-Ravens","year":"2014","journal-title":"Gastroenterology"},{"key":"ref271","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1136\/gutjnl-2012-303857","article-title":"Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia","volume":"63","author":"Vanheel","year":"2014","journal-title":"Gut"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1172\/JCI81129","article-title":"Extracellular vesicle isolation and characterization: toward clinical application","volume":"126","author":"Xu","year":"2016","journal-title":"J. Clin. 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Sci."},{"key":"ref131","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1136\/gutjnl-2011-300695","article-title":"Local barrier dysfunction identified by confocal laser endomicroscopy predicts relapse in inflammatory bowel disease","volume":"61","author":"Kiesslich","year":"2012","journal-title":"Gut"}],"container-title":["Physiology International"],"original-title":[],"link":[{"URL":"https:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/2060.106.2019.20","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/2060\/106\/3\/article-p225.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.akademiai.com\/doi\/pdf\/10.1556\/2060.106.2019.20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T02:43:55Z","timestamp":1644547435000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/doi\/10.1556\/2060.106.2019.20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":58,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/2060.106.2019.20","relation":{},"ISSN":["2498-602X"],"issn-type":[{"value":"2498-602X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]}}}