{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T16:43:54Z","timestamp":1776185034591,"version":"3.50.1"},"reference-count":100,"publisher":"Akademiai Kiado Zrt.","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["PhysInt"],"published-print":{"date-parts":[[2021,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n            <jats:sec>\n               <jats:title>Objective<\/jats:title>\n               <jats:p>To evaluate <jats:italic>in-vivo<\/jats:italic> and <jats:italic>in-vitro<\/jats:italic> effects of ferulic acid (FA) on glucocorticoid-induced osteoarthritis (GIO) to establish its possible underlying mechanisms.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>The effects of FA on cell proliferation, cell viability (MTT assay), ALP activity, and mineralization assay, and oxidative stress markers (ROS, SOD, GSH LDH and MDA levels) were investigated by MC3T3-E1 cell line. Wistar rats received standard saline (control group) or dexamethasone (GC, 2\u2009mg<jats:sup>\u22121<\/jats:sup>\u2009kg) or DEX+FA (50 and 100\u2009mg<jats:sup>\u22121<\/jats:sup>\u2009kg) orally for 8 weeks. Bone density, micro-architecture, bio-mechanics, bone turnover markers and histo-morphology were determined. The expression of OPG, RANKL, osteogenic markers, and other signalling proteins was assessed employing quantitative RT-PCR and Western blotting.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>The findings indicated the elevation of ALP mRNA expressions, osteogenic markers (Runx-2, OSX, Col-I, and OSN), and the \u03b2-Catenin, Lrp-5 and GSK-3\u03b2 protein expressions. FA showed the potential to increase MC3T3-E1 cell differentiation, proliferation, and mineralization. FA increased oxidative stress markers (SOD, MDA, and GSH) while decreasing ROS levels and lactate dehydrogenase release in GIO rats. The OPG\/RANKL mRNA expression ratio was increased by FA, followed by improved GSK-3\u03b2 and ERK phosphorylation with enhanced mRNA expressions of Lrp-5 and \u03b2-catenin.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusion<\/jats:title>\n               <jats:p>These findings showed that FA improved osteoblasts proliferation with oxidative stress suppression by controlling the Lrp-5\/GSK-3\u03b2\/ERK pathway in GIO, demonstrating the potential pathways involved in the mechanism of actions of FA in GIO therapy.<\/jats:p>\n            <\/jats:sec>","DOI":"10.1556\/2060.2021.00180","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T11:24:27Z","timestamp":1631791467000},"update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":6,"title":["Ferulic acid attenuates osteoporosis induced by glucocorticoid through regulating the GSK-3\u03b2\/Lrp-5\/ERK signalling pathways"],"prefix":"10.1556","author":[{"given":"Wei","family":"Zhou","sequence":"first","affiliation":[{"name":"Spinal and Trauma\u2019s Ward, The Third People Hospital of Dalian, Dalian City, 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"Spinal and Trauma\u2019s Ward, The Third People Hospital of Dalian, Dalian City, 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingbo","family":"Shang","sequence":"additional","affiliation":[{"name":"Spinal and Trauma\u2019s Ward, The Third People Hospital of Dalian, Dalian City, 116000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4065-870X","authenticated-orcid":true,"given":"Renbo","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref211","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/j.1872-034X.2012.01075.x","article-title":"Quercetin improves bone strength in experimental biliary cirrhosis","volume":"43","author":"Derakhshanian","year":"2013","journal-title":"Hepatol Res Off J Jpn Soc Hepatol"},{"key":"ref01","first-page":"113","article-title":"Osteoporosis: integrating biomarkers and other diagnostic correlates into the management of bone fragility","volume":"12","author":"McCormick","year":"2007","journal-title":"Altern Med Rev J Clin Ther"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1007\/s00776-013-0433-y","article-title":"Osteoprotective effect of propranolol in 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