{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T06:00:47Z","timestamp":1718604047584},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"20","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orvosi Hetilap"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p> Abstract: <\/jats:p><jats:p> Introduction: West Nile virus, a mosquito-borne viral zoonosis is responsible for human infections in Hungary. Laboratory diagnosis is based on serological tests, however the application of molecular methods has been appreciated. Aim: The aim of the study was to investigate blood, cerebrospinal-fluid and urine samples of acutely ill patients and to follow-up PCR positive cases to ascertain the length of virus excretion. Method: Clinical specimens were examined by indirect-immunofluorescent, haemagglutination-inhibition, two PCR tests and Sanger-sequencing. Virus isolation in case of two patients was successful. Results: A follow-up study could be carried out in case of 5 patients. Viral nucleic acid was detectable in urine even for several weeks after symptom onset and viral RNA was present at higher concentration compared with other samples. Conclusions: PCR analysis of urine could provide useful epidemiological and diagnostic information. Therefore, it is recommended to collect urine samples in order to supplement the serological diagnosis. Orv Hetil. 2017; 158(20): 791\u2013796. <\/jats:p>","DOI":"10.1556\/650.2017.30760","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T04:52:41Z","timestamp":1494823961000},"page":"791-796","source":"Crossref","is-referenced-by-count":1,"title":["A nyugat-n\u00edlusi v\u00edrus kimutat\u00e1sa hum\u00e1n betegmint\u00e1kb\u00f3l:                     nyomon k\u00f6vet\u00e9ses vizsg\u00e1latok a 2015. \u00e9vi szezon\u00e1lis id\u0151szakban"],"prefix":"10.1556","volume":"158","author":[{"given":"Anna","family":"Nagy","sequence":"first","affiliation":[{"name":"Virol\u00f3giai F\u0151oszt\u00e1ly, Orsz\u00e1gos Epidemiol\u00f3giai K\u00f6zpont Budapest, Albert Fl\u00f3ri\u00e1n \u00fat 2\u20136., 1097"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orsolya","family":"Nagy","sequence":"additional","affiliation":[{"name":"Virol\u00f3giai F\u0151oszt\u00e1ly, Orsz\u00e1gos Epidemiol\u00f3giai K\u00f6zpont Budapest, Albert 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