{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:07:59Z","timestamp":1785503279730,"version":"3.56.0"},"reference-count":9,"publisher":"Akademiai Kiado Zrt.","issue":"15","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.akjournals.com"],"crossmark-restriction":true},"short-container-title":["OH"],"published-print":{"date-parts":[[2026,4,12]]},"abstract":"<jats:p>\n                    <jats:italic>Bevezet\u00e9s:<\/jats:italic>\n                    A T-sejt-receptor-klonalit\u00e1s vizsg\u00e1lata r\u00e9g\u00f3ta\n                    alkalmazott rutin diagnosztikai m\u00f3dszer T-sejtes lymphoproliferati\u00f3kban, amely a\n                    T-sejt-receptor g\u00e9n\u00e1trendez\u0151d\u00e9s\u00e9nek vizsg\u00e1lat\u00e1n alapul. El\u0151nye a klonalit\u00e1s\n                    molekul\u00e1ris igazol\u00e1sa, h\u00e1tr\u00e1nya azonban \u2013 az \u00e1raml\u00e1si citometri\u00e1hoz k\u00e9pest \u2013 a\n                    kisebb \u00e9rz\u00e9kenys\u00e9g, a viszonylag hossz\u00fa vizsg\u00e1lati id\u0151tartam, valamint az, hogy\n                    nem ad fenot\u00edpusos inform\u00e1ci\u00f3t. A T-sejt-receptor-klonalit\u00e1s vizsg\u00e1lata egy \u00faj,\n                    nemzetk\u00f6zi aj\u00e1nl\u00e1s szerinti \u00e1raml\u00e1si citometriai m\u00f3dszerrel is v\u00e9gezhet\u0151. Ilyen\n                    egyszer\u0171s\u00edtett \u00e9s hat\u00e9kony megk\u00f6zel\u00edt\u00e9s kor\u00e1bban nem \u00e1llt rendelkez\u00e9sre a rutin\n                    klinikai diagnosztik\u00e1ban.\n                    <jats:italic>C\u00e9lkit\u0171z\u00e9s:<\/jats:italic>\n                    C\u00e9lunk az irodalomban\n                    le\u00edrt leg\u00fajabb aj\u00e1nl\u00e1s alapj\u00e1n a T-sejt-receptor konstans \u03b2-l\u00e1nc\u00e1hoz tervezett\n                    antitestek egy\u00fcttes haszn\u00e1lata sor\u00e1n szerzett tapasztalatok gy\u0171jt\u00e9se \u00e9s azok\n                    \u00f6sszevet\u00e9se a polimer\u00e1z-l\u00e1ncreakci\u00f3t alkalmaz\u00f3 g\u00e9n\u00e1trendez\u0151d\u00e9s-vizsg\u00e1latok\n                    eredm\u00e9nyeivel.\n                    <jats:italic>M\u00f3dszer:<\/jats:italic>\n                    A vizsg\u00e1lat alapja a T-sejt-receptor\n                    konstans \u03b2-l\u00e1nc\u00e1hoz tervezett, fluoreszcens fest\u00e9kkel konjug\u00e1lt k\u00e9tf\u00e9le\n                    monoklon\u00e1lis antitest alkalmaz\u00e1sa, amelyek \u2013 a B-sejtes neoplasi\u00e1k\n                    diagnosztiz\u00e1l\u00e1sakor haszn\u00e1latos kappa-lambda immunoglobulin-k\u00f6nny\u0171l\u00e1ncok\n                    vizsg\u00e1lat\u00e1hoz hasonlatosan \u2013 a norm\u00e1l expresszi\u00f3s ar\u00e1nyokt\u00f3l val\u00f3 elt\u00e9r\u00e9sen\n                    alapul.\n                    <jats:italic>Eredm\u00e9nyek:<\/jats:italic>\n                    Az 54 mint\u00e1b\u00f3l 20 esetben\n                    lymphoproliferativ betegs\u00e9g nem volt kimutathat\u00f3, 11 esetben tal\u00e1ltunk klon\u00e1lis\n                    popul\u00e1ci\u00f3t, amelyek j\u00f3 \u00f6sszhangban voltak a konvencion\u00e1lis vizsg\u00e1lati m\u00f3dszer\n                    eredm\u00e9nyeivel. Egy\u00e9b 23 esetben tal\u00e1ltunk kisebb fenot\u00edpusos elt\u00e9r\u00e9seket,\n                    sejtar\u00e1ny-eltol\u00f3d\u00e1sokat, minor monoklon\u00e1lis popul\u00e1ci\u00f3 jelenl\u00e9t\u00e9t.\n                    <jats:italic>Megbesz\u00e9l\u00e9s \u00e9s k\u00f6vetkeztet\u00e9s:<\/jats:italic>\n                    Az 54 minta eredm\u00e9nyei\n                    alapj\u00e1n elmondhat\u00f3, hogy a vizsg\u00e1lat nemcsak egy\u00e9rtelm\u0171 T- \u00e9s NKT-sejtes\n                    kl\u00f3noknak, hanem minor klon\u00e1lis popul\u00e1ci\u00f3knak az azonos\u00edt\u00e1s\u00e1ra is alkalmas, \u00e9s\n                    sz\u00e1mos esetben lehet\u0151v\u00e9 teszi a T-sejtes k\u00f3rk\u00e9pek gyorsabb diagn\u00f3zis\u00e1t. Orv\n                    Hetil. 2026; 167(15): 576\u2013584.\n                  <\/jats:p>","DOI":"10.1556\/650.2026.33522","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:38:12Z","timestamp":1776145092000},"page":"576-584","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["\u00daj klonalit\u00e1svizsg\u00e1lat a T-sejt-receptor b\u00e9ta-l\u00e1nc\u00e1nak konstans-1 \u00e9s -2 r\u00e9gi\u00f3j\u00e1ra specifikus antitestek haszn\u00e1lat\u00e1val T-sejtes lymphoproliferati\u00f3kban"],"prefix":"10.1556","volume":"167","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0374-9658","authenticated-orcid":true,"given":"Barbara","family":"Forr\u00f3","sequence":"first","affiliation":[{"name":"P\u00e9csi Tudom\u00e1nyegyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar, Klinikai K\u00f6zpont, Patol\u00f3giai Int\u00e9zet P\u00e9cs, Szigeti \u00fat 2., 7624 Magyarorsz\u00e1g"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Vida","sequence":"additional","affiliation":[{"name":"P\u00e9csi Tudom\u00e1nyegyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar, Klinikai K\u00f6zpont, Patol\u00f3giai Int\u00e9zet P\u00e9cs, Szigeti \u00fat 2., 7624 Magyarorsz\u00e1g"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Kereskai","sequence":"additional","affiliation":[{"name":"P\u00e9csi Tudom\u00e1nyegyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar, Klinikai K\u00f6zpont, Patol\u00f3giai Int\u00e9zet P\u00e9cs, Szigeti \u00fat 2., 7624 Magyarorsz\u00e1g"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1gnes","family":"Lacza","sequence":"additional","affiliation":[{"name":"P\u00e9csi Tudom\u00e1nyegyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar, Klinikai K\u00f6zpont, Patol\u00f3giai Int\u00e9zet P\u00e9cs, Szigeti \u00fat 2., 7624 Magyarorsz\u00e1g"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e1l","family":"J\u00e1ks\u00f3","sequence":"additional","affiliation":[{"name":"P\u00e9csi Tudom\u00e1nyegyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar, Klinikai K\u00f6zpont, Patol\u00f3giai Int\u00e9zet P\u00e9cs, Szigeti \u00fat 2., 7624 Magyarorsz\u00e1g"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Jiang H, Chess L. Regulation of immune responses by T cells. N Engl\n                    J Med. 2006; 354: 1166-1176.\n                    https:\/\/doi.org\/10.1056\/NEJMra055446","DOI":"10.1056\/NEJMra055446"},{"key":"R2","unstructured":"Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: the\n                    immune system in health and disease. 5th edition. Garland Science, New York, NY,\n                    2001. T-cell receptor gene rearrangement, 4. 11-14. Available from:\n                        https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK27145\/ [accessed: Jan 15\n                    2026]."},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Mahe E, Pugh T, Kamel-Reid S. T cell clonality assessment: past,\n                    present and future. J Clin Pathol. 2018; 71: 195-200. 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Cytometry B Clin Cytom.\n                    2024; 106: 192-202.\n                    https:\/\/doi.org\/10.1002\/cyto.b.22175","DOI":"10.1002\/cyto.b.22175"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Horna P, Weybright MJ, Ferrari M, et al. Dual T-cell constant \u03b2\n                    chain (TRBC)1 and TRBC2 staining for the identification of T-cell neoplasms by\n                    flow cytometry. Blood Cancer J. 2024; 14: 34.\n                        https:\/\/doi.org\/10.1038\/s41408-024-01002-0","DOI":"10.1038\/s41408-024-01002-0"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Trainor KJ, Brisco MJ, Wan JH, et al. Gene rearrangement in B- and\n                    T-lymphoproliferative disease detected by the polymerase chain reaction. Blood\n                    1991; 78: 192-196.\n                    https:\/\/doi.org\/10.1182\/blood.V78.1.192.192","DOI":"10.1182\/blood.V78.1.192.192"},{"key":"R8","doi-asserted-by":"crossref","unstructured":"Ginaldi L, Farahat N, Matutes E, et al. Differential expression of T\n                    cell antigens in normal peripheral blood lymphocytes: a quantitative analysis by\n                    flow cytometry. J Clin Pathol. 1996; 49: 539-544.\n                        https:\/\/doi.org\/10.1136\/jcp.49.7.539","DOI":"10.1136\/jcp.49.7.539"},{"key":"R9","doi-asserted-by":"crossref","unstructured":"Kov\u00e1cs S, Szab\u00f3 Z, Iv\u00e1nyi JL, et al. The diverse manifestations of\n                    T-cell large granular lymphocytic leukemia and successful treatment. [T-sejtes\n                    nagy granul\u00e1ris lymphocyt\u00e1s leuk\u00e9mia ezer arca \u00e9s sikeres kezel\u00e9se.] Hematol\n                    Transzfuziol. 2022; 55: 50-53. [Hungarian]\n                        https:\/\/doi.org\/10.1556\/2068.2022.00147","DOI":"10.1556\/2068.2022.00147"}],"container-title":["Orvosi Hetilap"],"original-title":[],"link":[{"URL":"https:\/\/www.akjournals.com\/view\/journals\/650\/167\/15\/article-p576.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.akjournals.com\/downloadpdf\/journals\/650\/167\/15\/article-p576.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.akjournals.com\/downloadpdf\/journals\/650\/167\/15\/article-p576.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T12:30:40Z","timestamp":1785501040000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.akjournals.com\/view\/journals\/650\/167\/15\/article-p576.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,12]]},"references-count":9,"journal-issue":{"issue":"15"},"URL":"https:\/\/doi.org\/10.1556\/650.2026.33522","relation":{},"ISSN":["0030-6002","1788-6120"],"issn-type":[{"value":"0030-6002","type":"print"},{"value":"1788-6120","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,12]]}}}