{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T00:10:04Z","timestamp":1686010204427},"reference-count":23,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:p>Methicillin-resistant<jats:italic>Staphylococcus aureus<\/jats:italic>(MRSA), particularly the multidrug-resistant clones, is an increasing worldwide problem. The average incidence rate of MRSA in Tehran was found to be over 40%. A total of 140 MRSA isolates obtained from patients attending a teaching hospital in Tehran, from May 2009 to December 2009, were included in this study. The antimicrobial susceptibility profile of MRSA isolates was determined by the agar disk diffusion method. Molecular analysis of MRSA strains was accomplished by Pulsed-Field Gel Electrophoresis (PFGE) and Multi-locus sequence typing (MLST). Detection of<jats:italic>mecA<\/jats:italic>gene was used to confirm resistance to methicillin among the MRSA isolates. All the MRSA isolates were susceptible to chloramphenicol, teicoplanin, tigecycline and vancomycin. All MRSAisolates were resistant to oxacillin, whilst 139 strains showed resistance against ciprofloxacin, erythromycin, gentamicin, tetracycline and trimethoprim-sulfamethoxazole. PFGE analysis of all the 140 MRSA isolates produced five distinct pulsotypes designated as pulsotypes A-E. Most of the isolates (n=132) were clustered into pulsotype A. The most prevalent sequence type (ST) was ST 239 (pulsotype A) found in 82% (37\/45) of the tested isolates. The second most prevalent type was ST 1238 (pulsotypes B, C and D) found in 15% (7\/45) of the isolates. The remaining type, ST 8 (pulsotype E) was found in a single isolate. The results of this study indicated that the MRSA clone ST 239 was a major clone in the selected university hospital of Tehran and that it was widely spread among the different wards as well as all the age groups of patients.<\/jats:p>","DOI":"10.1556\/amicr.58.2011.1.4","type":"journal-article","created":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T02:26:06Z","timestamp":1301538366000},"page":"31-39","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":17,"title":["Molecular analysis and antimicrobial susceptibility of methicillin resistant Staphylococcus aureus in one of the hospitals of Tehran University of Medical Sciences: High prevalence of sequence type 239 (ST239) clone"],"prefix":"10.1556","volume":"58","author":[{"given":"Shadi","family":"Shahsavan","sequence":"first","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Jabalameli","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Maleknejad","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marzieh","family":"Aligholi","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Imaneini","sequence":"additional","affiliation":[{"name":"2 University of Tehran ECE Department, Engineering Faculty Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fereshteh","family":"Jabalameli","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahnaz","family":"Halimi","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morovat","family":"Taherikalani","sequence":"additional","affiliation":[{"name":"3 Ilam University of Medical Sciences Department of Microbiology, School of Medicine Ilam Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Babak","family":"Khoramian","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Eslampour","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Feizabadi","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Emaneini","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"3610","DOI":"10.1128\/JCM.43.8.3610-3614.2005","article-title":"Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital","volume":"43","author":"Cha H. 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