{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T16:05:10Z","timestamp":1704816310865},"reference-count":20,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:p>Previous studies showed that methylxanthines increased the antimicrobial activity of gentamicin against <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. In this study, the effect of non-selective phosphodiesterase (PDE) inhibitors (methylxanthines: aminophylline and caffeine) and partially selective PDE inhibitors, dipyridamole and sildenafil, was evaluated on the antimicrobial activity of gentamicin using checkerboard method. Aminophylline at concentrations of 0.5 and 1 mg\/ml reduced the minimal inhibitory concentration (MIC) of gentamicin (2 \u03bcg\/ml) 2 and 4 times against <jats:italic>S. aureus<\/jats:italic>, and at concentrations of 0.5 and 2 mg\/ml reduced the MIC of gentamicin (4 \u03bcg\/ml) 2 and 4 times, respectively, against <jats:italic>P. aeruginosa<\/jats:italic>. Caffeine at concentrations of 1 and 2 mg\/ml reduced the MIC of gentamicin (2 \u03bcg\/ml) 4 and 32 times against <jats:italic>S. aureus<\/jats:italic>, and at concentrations of 0.12 and 2 mg\/ml reduced the MIC of gentamicin (4 \u03bcg\/ml) 2 and 4 times, respectively, against <jats:italic>P. aeruginosa<\/jats:italic>. However, dipyridamole and sildenafil (32 \u03bcg\/ml) did not show any effect on MIC of gentamicin against <jats:italic>S. aureus<\/jats:italic> and <jats:italic>P. aeruginosa<\/jats:italic>. These results suggest that methylxanthines could increase gentamicin effects against <jats:italic>S. aureus<\/jats:italic> and <jats:italic>P. aeruginosa<\/jats:italic> but this effect is not mediated by inhibition of PDE 5, 6, 8, 10 and 11.<\/jats:p>","DOI":"10.1556\/amicr.59.2012.1.2","type":"journal-article","created":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T01:09:18Z","timestamp":1334711358000},"page":"13-20","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":4,"title":["Interaction of methylxanthines and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: Role of phosphodiesterase inhibition"],"prefix":"10.1556","volume":"59","author":[{"given":"Bibi","family":"Bazzaz","sequence":"first","affiliation":[{"name":"1 Mashhad University of Medical Sciences Biotechnology Research Center, School of Pharmacy Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shokoufeh","family":"Lavaei","sequence":"additional","affiliation":[{"name":"2 School, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Hosseinzadeh","sequence":"additional","affiliation":[{"name":"3 Mashhad University of Medical Sciences Pharmaceutical Research Center, School of Pharmacy Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1007\/s00018-007-7051-9","article-title":"Caffeine analogs: Biomedical impact","volume":"64","author":"Daly J. 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