{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:39:00Z","timestamp":1773283140317,"version":"3.50.1"},"reference-count":23,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:p>Staphylococcal infections have high occurrence in Jordanian patients. This study was carried out to determine the rates of high- and low-level mupirocin resistance (MupH and MupL) among staphylococci with the molecular characterization. Two hundred and thirty-two non-duplicate<jats:italic>Staphylococcus<\/jats:italic>spp. isolated from different clinical specimens were tested for mupirocin susceptibility using disk diffusion method and minimum inhibitory concentration (MIC). Resistance genes and clone relatedness was studied using polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus primers (Eric-PCR) for the latter. Plasmid curing was performed to determine the genetic location of<jats:italic>MupA<\/jats:italic>gene. Among the 232 strains, 144 (62%) were methicillin-resistant<jats:italic>Staphylococcus aureus<\/jats:italic>(MRSA), 33 (14.2%) methicillin-susceptible<jats:italic>Staphylococcus aureus<\/jats:italic>(MSSA) and 55 (23.7%) were of other coagulase-negative<jats:italic>Staphylococcus<\/jats:italic>spp. (CoNS). Of all strains tested, only 6 (2.6%) were mupirocin resistant.<jats:italic>MecA<\/jats:italic>gene was detected in both MupL and MupH strains but<jats:italic>MupA<\/jats:italic>gene was only detected in MupH. Plasmid curing improved the plasmidic location of<jats:italic>MupA<\/jats:italic>gene. Molecular typing by Eric-PCR method revealed heterogenicity of the genetic make up of our MupL and MupH strains. Staphylococci with<jats:italic>MupA<\/jats:italic>-carrying genes are present in Jordanian hospitals, but thank to the limited use of mupirocin, they remain rare.<\/jats:p>","DOI":"10.1556\/amicr.59.2012.2.8","type":"journal-article","created":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T19:31:51Z","timestamp":1341257511000},"page":"239-247","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":21,"title":["Rare occurrence of mupirocin resistance among clinical Staphylococcus isolates in Jordan"],"prefix":"10.1556","volume":"59","author":[{"given":"Amin","family":"Aqel","sequence":"first","affiliation":[{"name":"1 Mu\u2019tah University Faculty of Medicine Al-Karak Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdallah","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"2 Zarqa University Faculty of Allied Medical Sciences Zarqa Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asem","family":"Shehabi","sequence":"additional","affiliation":[{"name":"3 Jordan University Faculty of Medicine Amman Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1089\/mdr.2004.10.321","article-title":"Antibiotic resistance patterns of mecA-positive Staphylococcus aureus isolates from clinical specimens and nasal carriage","volume":"10","author":"Al-Zu\u2019bi E.","year":"2004","journal-title":"Microb. Drug. Resist."},{"key":"2","first-page":"310","article-title":"ARMed Project Members and Collaborators: Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in invasive isolates from southern and eastern Mediterranean countries","volume":"60","author":"Borg M. A.","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"3","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1574-695X.2000.tb01417.x","article-title":"Frequency of nasal and wound isolates of Staphylococcus aureus associated with TSST-1 production in Jordanian population","volume":"27","author":"Daghistani H. I.","year":"2000","journal-title":"FEMS Immunol. Med. Microbio."},{"key":"4","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.idc.2010.11.002","article-title":"Control of methicillin-resistant Staphylococcus aureus","volume":"25","author":"Lee A. S.","year":"2011","journal-title":"Infect. Dis. Clin. North Am."},{"key":"5","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.ajic.2010.07.008","article-title":"Clinical and cost ineffectiveness of preoperative screening for methicillin-resistant Staphylococcus aureus and intranasal mupirocin in preventing methicillin-resistant S aureus infections in cardiothoracic surgery","volume":"39","author":"Cunha B. A.","year":"2011","journal-title":"Am. J. Infect. Control."},{"key":"6","doi-asserted-by":"crossref","first-page":"372","DOI":"10.4103\/0255-0857.71825","article-title":"Mupirocin resistance in clinical isolates of staphylococci in a tertiary care centre in south India","volume":"28","author":"Oommen S. K.","year":"2010","journal-title":"Indian J. Med. Microbiol."},{"key":"7","doi-asserted-by":"crossref","first-page":"4158","DOI":"10.1128\/JCM.01022-09","article-title":"Acquisition of high-level mupirocin resistance and its fitness cost among methicillin-resistant Staphylococcus aureus strains with low-level mupirocin resistance","volume":"47","author":"Mongkolrattanothai K.","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"8","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.ijantimicag.2003.11.007","article-title":"Paul-Ehrlich-Society for Chemotherapy: Prevalence of mupirocin resistance in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis: Results of the Antimicrobial Resistance Surveillance Study of the Paul-Ehrlich-Society for Chemotherapy 2001","volume":"23","author":"Kresken M.","year":"2004","journal-title":"Int. J. Antimicrob. Agents"},{"key":"9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jac\/42.4.489","article-title":"The prevalence of low- and high-level mupirocin resistance in staphylococci from 19 European hospitals","volume":"42","author":"Schmitz F. J.","year":"1998","journal-title":"J. Antimicrob. Chemother."},{"key":"10","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1179\/joc.1999.11.5.331","article-title":"Emergence of resistance during mupirocin treatment: is it a problem in clinical practice?","volume":"11","author":"Henkel T.","year":"1999","journal-title":"J. Chemother."},{"key":"11","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1128\/AAC.43.6.1412","article-title":"Randomized, placebo-controlled, double-blind trial to evaluate the efficacy of mupirocin for eradicating carriage of methicillin-resistant Staphylococcus aureus","volume":"43","author":"Harbarth S.","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"12","volume-title":"Performance standards for antimicrobial susceptibility testing. Seventeenth informational supplement. M100-S17","year":"2007"},{"key":"13","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1099\/jmm.0.46965-0","article-title":"Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus","volume":"56","author":"Oliveira N. E.","year":"2007","journal-title":"J. Med. Microbiol."},{"key":"14","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1128\/AAC.41.5.1137","article-title":"Interpretive criteria for testing susceptibility of staphylococci to mupirocin","volume":"41","author":"Finlay J. E.","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"15","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0924-8579(01)00429-0","article-title":"Mupirocin resistance in Staphylococcus aureus from Greek hospitals","volume":"18","author":"Legakis N. J.","year":"2001","journal-title":"Int. J. Antimicrob. Agents"},{"key":"16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0732-8893(01)00262-0","article-title":"Correlation of MIC methods and tentative interpretive criteria for disk diffusion susceptibility testing using NCCLS methodology for fusidic acid","volume":"40","author":"Skov R.","year":"2001","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"17","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1128\/AAC.37.1.32","article-title":"High-level mupirocin resistance in Staphylococcus aureus: Evidence for two distinct isoleucyl-tRNA synthetases","volume":"37","author":"Gilbart J.","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"key":"18","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1128\/JCM.37.9.2781-2788.1999","article-title":"Outbreak of mupirocin-resistant staphylococci in a hospital in Warsaw, Poland, due to plasmid transmission and clonal spread of several strains","volume":"37","author":"Leski T. A.","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"19","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0924-8579(01)00327-2","article-title":"Bacterial Resistance Study Group of Thessaly: Methicillin-resistant Staphylococcus aureus in the hospitals of central Greece","volume":"18","author":"Petinaki E.","year":"2001","journal-title":"Int. J. Antimicrob. Agents"},{"key":"20","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1099\/0022-1317-50-10-909","article-title":"Genetic analysis of methicillin-resistant Staphylococcus aureus expressing high- and low-level mupirocin resistance","volume":"50","author":"Udo E. E.","year":"2001","journal-title":"J. Med. Microbiol."},{"key":"21","doi-asserted-by":"crossref","first-page":"6823","DOI":"10.1093\/nar\/19.24.6823","article-title":"Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes","volume":"19","author":"Versalovic J.","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"22","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1128\/JCM.00759-10","article-title":"Association of high-level mupirocin resistance and multidrug-resistant methicillin-resistant Staphylococcus aureus at an academic center in the midwestern United States","volume":"49","author":"Cadilla A.","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"23","doi-asserted-by":"crossref","first-page":"286","DOI":"10.7883\/yoken.63.286","article-title":"Prevalence of mupirocin resistance in methicillin-resistant Staphylococcus aureus strains isolated from a Malaysian hospital","volume":"63","author":"Lim K. T.","year":"2010","journal-title":"Jpn. J. Infect. Dis."}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/59\/2\/article-p239.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/59\/2\/article-p239.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T01:17:53Z","timestamp":1687569473000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/59\/2\/article-p239.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,1]]},"references-count":23,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/amicr.59.2012.2.8","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,1]]}}}