{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T01:05:34Z","timestamp":1771722334990,"version":"3.50.1"},"reference-count":29,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p>\n          <jats:italic>Legionella pneumophila<\/jats:italic> is an important etiological agent in both hospital and community acquired pneumonia. The sensitivity of culture for isolation of <jats:italic>L. pneumophila<\/jats:italic> from clinical specimens is low and time consuming. Similar problem also exists when the method of direct immunofluorescence is used. To detect this organism quantitatively from respiratory specimens, a Taq Man based real-time PCR targeting the <jats:italic>mip<\/jats:italic> sequence was developed. Both real-time PCR and culture methods were applied on 262 respiratory specimens from 262 ICU patients with pneumonia admitted to 5 different hospitals in Tehran. The results of real-time PCR were compared with those obtained by culture. Real-time PCR and culture found 12 and 4 specimens, respectively, as positive for <jats:italic>L. pneumophila<\/jats:italic>. Its technical specificity (100%) was checked against a panel of microorganisms consisting of both Gram-positive and Gram-negative bacteria. Our real-time PCR assay showed high sensitivity (100%) and specificity (96.9%) and could detect 200 organisms per ml from respiratory specimens. Using real-time PCR as a screening method, the frequency of nosocomial pneumonia with <jats:italic>L. pneumophila<\/jats:italic> at Tehran hospitals was estimated as 4.58%.<\/jats:p>","DOI":"10.1556\/amicr.59.2012.3.6","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T10:30:39Z","timestamp":1347705039000},"page":"355-365","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":13,"title":["Hospital acquired pneumonia: Comparison of culture and real-time PCR assays for detection of Legionella pneumophila from respiratory specimens at Tehran Hospitals"],"prefix":"10.1556","volume":"59","author":[{"given":"Somayeh","family":"Fard","sequence":"first","affiliation":[{"name":"1 Tehran University of Medical of Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bizhan","family":"Nomanpour","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical of Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahram","family":"Fatolahzadeh","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical of Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraf","family":"Mobarez","sequence":"additional","affiliation":[{"name":"2 Tarbiat Modares University Department of Bacteriology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davood","family":"Darban-Sarokhalil","sequence":"additional","affiliation":[{"name":"3 Shahrekord University of Medical Sciences Department of Microbiology Shahrekord Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Fooladi","sequence":"additional","affiliation":[{"name":"4 Baqiyatallah University of Medical Sciences Applied Microbiology Research Center Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willem","family":"Leeuwen","sequence":"additional","affiliation":[{"name":"5 Erasmus MC Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Feizabadi","sequence":"additional","affiliation":[{"name":"1 Tehran University of Medical of Sciences Department of Microbiology, School of Medicine Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinf.2007.09.010","article-title":"Legionella spp. and Legionnaires\u2019 disease","volume":"56","author":"Diederen B. 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