{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T13:56:22Z","timestamp":1780840582293,"version":"3.54.1"},"reference-count":26,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan\u00ae probes to detect<jats:italic>Salmonella<\/jats:italic>Typhi. TaqMan\u00ae real-time PCR assays were performed by designed primers and probe based on the<jats:italic>staG<\/jats:italic>gene for detecting<jats:italic>S.<\/jats:italic>Typhi. The specificity of the assay was evaluated on 15<jats:italic>Salmonella<\/jats:italic>serovars. The analytical specificity was evaluated on 20 non-<jats:italic>Salmonella<\/jats:italic>microorganisms. The analytical sensitivity was assessed using decreasing DNA quantities of<jats:italic>S.<\/jats:italic>Typhi ATCC 19430. Finally the detection capability of the TaqMan\u00ae real-time PCR assay on isolates recovered from patients with<jats:italic>Salmonella<\/jats:italic>infections was compared to the conventional PCR assay. Only<jats:italic>S.<\/jats:italic>Typhi strain had positive results when subjected to the assay using Typhi-specific real-time PCR. No amplification products were observed in real-time PCR with any of the non-<jats:italic>Salmonella<\/jats:italic>microorganisms tested. The TaqMan\u00ae real-time PCR was more sensitive than the conventional PCR. In conclusion, we found that the easy-to-use real-time PCR assays were faster than conventional PCR systems. The<jats:italic>staG<\/jats:italic>-based TaqMan\u00ae real-time PCR assay showed to be specific and sensitive method for the safe and rapid detection of the<jats:italic>S.<\/jats:italic>Typhi.<\/jats:p>","DOI":"10.1556\/amicr.61.2014.2.3","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T01:05:38Z","timestamp":1403053538000},"page":"121-130","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":12,"title":["Use of TaqMan\u00ae real-time PCR for rapid detection of Salmonella enterica serovar Typhi"],"prefix":"10.1556","volume":"61","author":[{"given":"Reza","family":"Ranjbar","sequence":"first","affiliation":[{"name":"1 Baqiyatallah University of Medical Sciences Molecular Biology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Naghoni","sequence":"additional","affiliation":[{"name":"1 Baqiyatallah University of Medical Sciences Molecular Biology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohreh","family":"Farshad","sequence":"additional","affiliation":[{"name":"2 Shiraz University of Medical Sciences Prof. Alborzi Clinical Microbiology Research Center Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Lashini","sequence":"additional","affiliation":[{"name":"3 Baqiyatallah University of Medical Sciences Applied Virology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Najafi","sequence":"additional","affiliation":[{"name":"1 Baqiyatallah University of Medical Sciences Molecular Biology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourkhoda","family":"Sadeghifard","sequence":"additional","affiliation":[{"name":"4 Ilam University of Medical Sciences Clinical Microbiology Research Center Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Mammina","sequence":"additional","affiliation":[{"name":"5 Department of Sciences for Health Promotion and Mother-Child Care \u2018G. D\u2019Alessandro\u2019, University Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1089\/fpd.2007.0002","article-title":"The attribution of human infections with antimicrobial resistant Salmonella bacteria in Denmark to sources of animal origin","volume":"4","author":"Hald T.","year":"2007","journal-title":"Food-Borne Patho Dis"},{"key":"2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.7883\/yoken.63.417","article-title":"High prevalence of integron-mediated resistances among clinical isolates of Salmonella enterica","volume":"63","author":"Naghoni A.","year":"2010","journal-title":"Jpn J of Infect Dis"},{"key":"3","volume-title":"World Health Organization antimicrobial resistance fact sheet no. 139","year":"2003"},{"key":"4","first-page":"38","article-title":"Rapid detection of different serovares of Salmonella enterica by multiplex PCR","volume":"36","author":"Karami A.","year":"2007","journal-title":"Iran J Public Health"},{"key":"5","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ijfoodmicro.2004.02.013","article-title":"Application of a molecular beacon \u2014 real-time PCR technology to detect Salmonella species contaminating fruits and vegetables","volume":"95","author":"Liming S.H.","year":"2004","journal-title":"Int J Food Microbiol"},{"key":"6","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.4315\/0362-028X-70.5.1080","article-title":"A real-time PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices","volume":"70","author":"Bohaychuk V.M.","year":"2007","journal-title":"J Food Prot"},{"key":"7","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/BF02740817","article-title":"Impact of molecular biology on the detection of foodborne pathogens","volume":"7","author":"Feng P.","year":"1997","journal-title":"Mol Biotechnol"},{"key":"8","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.2460\/ajvr.2002.63.1265","article-title":"Detection of Salmonella spp in fecal specimens by use of real-time polymerase chain reaction assay","volume":"63","author":"Kurowski P.B.","year":"2002","journal-title":"Am J Vet Res"},{"key":"9","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.ijfoodmicro.2009.12.004","article-title":"A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis","volume":"137","author":"Chen J.","year":"2010","journal-title":"Int J Food Microbiol"},{"key":"10","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0168-1605(99)00209-3","article-title":"Combined PCR and slot blot assay for detection of Salmonella and Listeria monocytogenes","volume":"56","author":"Li X.","year":"2000","journal-title":"Int J Food Microbiol"},{"key":"11","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/sj.jim.2900520","article-title":"Simultaneous detection of Salmonella spp. and Escherichia coli O157:H7 by multiplex PCR","volume":"21","author":"Fratamico P.","year":"1998","journal-title":"J Ind Microbiol Biotech"},{"key":"12","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1006\/abio.2000.4518","article-title":"Molecular beacons: A real-time polymerase chain reaction assay for detecting Salmonella","volume":"280","author":"Chen W.","year":"2000","journal-title":"Anal Biochem"},{"key":"13","doi-asserted-by":"crossref","first-page":"3429","DOI":"10.1128\/JCM.38.9.3429-3435.2000","article-title":"Automated 5\u2032 nuclease PCR assay for identification of Salmonella enterica","volume":"38","author":"Hoorfar J.","year":"2000","journal-title":"J Clin Microbiol"},{"key":"14","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/nbt0396-303","article-title":"Molecular beacon: Probes that fluoresce upon hybridization","volume":"14","author":"Tyagi S.","year":"1996","journal-title":"Nat Biotechnol"},{"key":"15","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0168-1605(97)01241-5","article-title":"The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities","volume":"35","author":"Chen S.","year":"1997","journal-title":"Int J Food Microbiol"},{"key":"16","doi-asserted-by":"crossref","first-page":"855","DOI":"10.4315\/0362-028X-63.7.855","article-title":"Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk","volume":"63","author":"McKllip J.L.","year":"2000","journal-title":"J Food Prot"},{"key":"17","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.vetmic.2008.12.023","article-title":"Real-time PCR for identification of Brucella spp.: A comparative study of IS711, bcsp31 and per target genes","volume":"137","author":"Bounaadja L.","year":"2009","journal-title":"Vet Microbiol"},{"key":"18","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1586\/14737159.5.2.209","article-title":"Basic principles of real-time quantitative PCR","volume":"5","author":"Arya M.","year":"2005","journal-title":"Expert Rev Mol Diagn"},{"key":"19","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1574-6941.2008.00629.x","article-title":"Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology","volume":"67","author":"Smith C.J.","year":"2009","journal-title":"FEMS Microbiol Ecol"},{"key":"20","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1309\/DP0HY5UT10HQW9YM","article-title":"Broad-range (Pan) Salmonella and Salmonella serotype Typhi-specific real-time PCR assays","volume":"123","author":"Farrell J.J.","year":"2005","journal-title":"Am J Clin Pathol"},{"key":"21","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.4315\/0362-028X-66.10.1762","article-title":"Using a portable real-time PCR assay to detect Salmonella in raw milk","volume":"66","author":"Kessel J.S.","year":"2003","journal-title":"J Food Prot"},{"key":"22","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/1471-2334-10-125","article-title":"The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens","volume":"10","author":"Nga T.V.","year":"2010","journal-title":"BMC Infect Dis"},{"key":"23","first-page":"1815","article-title":"Using current molecular techniques for rapid differentiation of Salmonella Typhi and Salmonella Typhimurium","volume":"8","author":"Jordan R.","year":"2009","journal-title":"Afr J Biotechnol"},{"key":"24","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/JCM.33.3.775-777.1995","article-title":"Development of nested PCR based on the ViaB sequence to detect Salmonella typhi","volume":"33","author":"Hashimoto Y.","year":"1995","journal-title":"J Clin Microbiol"},{"key":"25","doi-asserted-by":"crossref","first-page":"612","DOI":"10.5897\/AJB09.1761","article-title":"Using molecular techniques for rapid detection of Salmonella serovars in frozen chicken and chicken products collected from Riyadh, Saudi Arabia","volume":"9","author":"Moussa I.M.","year":"2010","journal-title":"Afr J Biotechnol"},{"key":"26","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s12161-010-9142-8","article-title":"Validation of a duplex real-time PCR for the detection of Salmonella spp. in different food products","volume":"4","author":"Anderson A.","year":"2011","journal-title":"Food Anal Methods"}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/61\/2\/article-p121.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/61\/2\/article-p121.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T16:29:59Z","timestamp":1649435399000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/61\/2\/article-p121.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,1]]},"references-count":26,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/amicr.61.2014.2.3","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,1]]}}}