{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T05:52:48Z","timestamp":1677736368435},"reference-count":33,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>Despite concerted efforts, diagnosis of aspergillosis is still a great challenge to clinical microbiology laboratories. Along with the requirement for high sensitivity and specificity, species-specific identification is important. We developed rapid, sensitive and species-specific qPCR assays using the TaqMan technology for the detection and identification of<jats:italic>Aspergillus fumigatus<\/jats:italic>and<jats:italic>Aspergillus terreus<\/jats:italic>. The assays were designed to target orthologs of the<jats:italic>Streptomyces<\/jats:italic>factor C gene that are only found in a few species of filamentous fungi. Fungi acquired this gene through horizontal gene transfer and divergence of the gene allows identification of species. The assays have potential as a molecular diagnosis tool for the early detection of fungal infection caused by<jats:italic>Aspergillus fumigatus<\/jats:italic>and<jats:italic>Aspergillus terreus<\/jats:italic>, which merits future diagnostic studies. The assays were sensitive enough to detect a few genomic equivalents in blood samples.<\/jats:p>","DOI":"10.1556\/amicr.61.2014.3.3","type":"journal-article","created":{"date-parts":[[2014,9,28]],"date-time":"2014-09-28T01:03:00Z","timestamp":1411866180000},"page":"273-284","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":2,"title":["Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species"],"prefix":"10.1556","volume":"61","author":[{"given":"Melinda","family":"Paholcsek","sequence":"first","affiliation":[{"name":"1 University of Debrecen Department of Human Genetics, Faculty of Medicine Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9va","family":"Leiter","sequence":"additional","affiliation":[{"name":"2 University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen 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