{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:58:33Z","timestamp":1782734313648,"version":"3.54.5"},"reference-count":25,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:p>\n          <jats:italic>Moniezia benedeni<\/jats:italic> and <jats:italic>M. expansa<\/jats:italic> are common ruminant tapeworms of worldwide distribution, causing gastrointestinal disorders and even death in sheep and goats. In this study, a polymerase chain reaction- (PCR-) based approach for precise species identification was developed and also applied to faecal DNA diagnosis of the tapeworm infection. Since nuclear ribosomal DNA (rDNA) appears to be a useful target for species and\/or strain markers, the 18S regions of the rDNA of <jats:italic>M. benedeni<\/jats:italic> and <jats:italic>M. expansa<\/jats:italic> were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2476\u20132487 bp and 51.9\u201352.1% for <jats:italic>M. benedeni<\/jats:italic> and 2473 bp and 51.9\u201352.0% for <jats:italic>M. expansa<\/jats:italic>, respectively. Alignment and comparison of the 18S sequences of the two species showed 92.5\u201393.3% homology. No matches for the 18S regions of <jats:italic>M. benedeni<\/jats:italic> and <jats:italic>M. expansa<\/jats:italic> were found with other species by BLAST search, which made the 18S sequences appropriate markers for the design of distinctive primers for the two <jats:italic>Moniezia<\/jats:italic> species. Our 18S-based PCR could detect as low levels as 0.5 pg genomic DNA or the DNA extracted from 0.2 g faecal sample collected from the rectum of an <jats:italic>M. expansa<\/jats:italic>-infected goat. The results indicate that this PCR approach is a reliable alternative for the differential diagnosis of <jats:italic>Moniezia<\/jats:italic> species in faecal samples.<\/jats:p>","DOI":"10.1556\/avet.2013.035","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T01:06:45Z","timestamp":1374109605000},"page":"463-472","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":16,"title":["Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA)"],"prefix":"10.1556","volume":"61","author":[{"given":"HongBin","family":"Yan","sequence":"first","affiliation":[{"name":"1 CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XinWen","family":"Bo","sequence":"additional","affiliation":[{"name":"2 Xinjiang Academy of Agricultural and Reclamation Science The Breed and Biotechnology Key Laboratory of Sheep Shihezi P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youyu","family":"Liu","sequence":"additional","affiliation":[{"name":"1 CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongzi","family":"Lou","sequence":"additional","affiliation":[{"name":"1 CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XingWei","family":"Ni","sequence":"additional","affiliation":[{"name":"1 CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WanGui","family":"Shi","sequence":"additional","affiliation":[{"name":"3 Gansu Provincial Control for Animal Disease Control and Prevention Lanzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Zhan","sequence":"additional","affiliation":[{"name":"3 Gansu Provincial Control for Animal Disease Control and Prevention Lanzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HongKean","family":"Ooi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WanZhong","family":"Jia","sequence":"additional","affiliation":[{"name":"1 CAAS State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Zoonoses of the Ministry of Agriculture, Lanzhou Veterinary Research Institute Lanzhou, Gansu Province 730046 The People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"1","unstructured":"Baer, J. G. (1927): Monographie des cestodes de la famille des Anoplocephalides. Bull. Biol. 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