{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:10:56Z","timestamp":1791360656484,"version":"4.3.1"},"reference-count":19,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>Infectious bursal disease virus is an important poultry pathogen. It is distributed worldwide and causes significant economic losses. In this study, a system was adopted for the simultaneous monitoring of vaccine and virulent strains using reverse transcription polymerase chain reaction (RT-PCR). After the decay of maternal antibodies, chickens were vaccinated at the age of 37 days with a virus of intermediate virulence and challenged at 5, 10 and 14 days post vaccination (dpv). The challenge was done with IBDV strain CH\/99. Sequencing of the hypervariable region of VP2 has shown that CH\/99 belongs to the very virulent group of viruses. The vaccine virus could be found in the bursa of Fabricius, spleen, thymus and bone marrow until 24 dpv. The CH\/99 challenge virus was found in the bursa and lymphoid organs when chickens were challenged at 5 and 10 dpv. When challenge was performed at 14 dpv, the pathogenic virus could not be found in the bursa and other lymphoid organs.<\/jats:p>","DOI":"10.1556\/avet.2014.003","type":"journal-article","created":{"date-parts":[[2014,3,23]],"date-time":"2014-03-23T01:09:59Z","timestamp":1395536999000},"page":"264-273","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":4,"title":["Simultaneous detection of vaccinal and field infectious bursal disease viruses in layer chickens challenged with a very virulent strain after vaccination"],"prefix":"10.1556","volume":"62","author":[{"given":"Ivan","family":"Dobrosavljevi\u0107","sequence":"first","affiliation":[{"name":"1 Veterinary Institute Po\u017earevac Po\u017earevac Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dejan","family":"Vidanovi\u0107","sequence":"additional","affiliation":[{"name":"2 Veterinary Institute Kraljevo Kraljevo Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Velhner","sequence":"additional","affiliation":[{"name":"3 Scientific Veterinary Institute \u2018Novi Sad\u2019 Rumena\u010dki put 20 Novi Sad Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biljana","family":"Miljkovi\u0107","sequence":"additional","affiliation":[{"name":"4 Research Veterinary Institute of Serbia Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branislav","family":"Lako","sequence":"additional","affiliation":[{"name":"5 University of Novi Sad Faculty of Agriculture, Department for Veterinary Medicine Novi Sad Novi Sad Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0042-6822(87)90180-2","article-title":"Deletion mapping and expression in Escherichia coli of the large genomic segment of birnavirus","volume":"161","author":"Azad A. 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Res."},{"key":"4","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1637\/9671-012811-Reg.1","article-title":"Viral competition and maternal immunity influence the clinical disease caused by very virulent infectious bursal disease virus","volume":"55","author":"Jackwood D. J.","year":"2011","journal-title":"Avian Dis."},{"key":"5","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1637\/7370-042905R.1","article-title":"Detection of infectious bursal disease virus in various lymphoid tissue of experimentally infected specific pathogen free chickens by different reverse transcription polymerase chain reaction assays","volume":"49","author":"Kabell S.","year":"2005","journal-title":"Avian Dis."},{"key":"6","doi-asserted-by":"crossref","first-page":"8884","DOI":"10.1128\/JVI.74.19.8884-8892.2000","article-title":"Characteristics of bursal T lymphocytes induced by infectious bursal disease virus","volume":"74","author":"Kim I. J.","year":"2000","journal-title":"J. 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Paris, 2008. pp. 549\u2013565","year":"2008"},{"key":"11","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1637\/7310-112204R","article-title":"Protective efficacy of intermediate and intermediate plus infectious bursal disease virus (IBDV) vaccines against very virulent IBDV in commercial broilers","volume":"49","author":"Rautenschlein S.","year":"2005","journal-title":"Avian Dis."},{"key":"12","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s705-002-8320-2","article-title":"Role of intrabursal T cells in infectious bursal disease virus (IBDV) infection: T cells promote viral clearance but delay follicular recovery","volume":"147","author":"Rautenschlein S.","year":"2002","journal-title":"Arch. Virol."},{"key":"13","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1007\/s00705-002-0817-3","article-title":"Characterization of an Indonesian very virulent strain of infectious bursal disease virus","volume":"147","author":"Rudd M. 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Virol."},{"key":"14","doi-asserted-by":"crossref","first-page":"638","DOI":"10.2307\/1592155","article-title":"Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens","volume":"41","author":"Tanimura N.","year":"1997","journal-title":"Avian Dis."},{"key":"15","doi-asserted-by":"crossref","first-page":"9","DOI":"10.2307\/1591976","article-title":"Association between pathogenicity of infectious bursal disease virus and viral antigen distribution detected by immunohistochemistry","volume":"39","author":"Tanimura N.","year":"1995","journal-title":"Avian Dis."},{"key":"16","doi-asserted-by":"crossref","first-page":"844","DOI":"10.2307\/1592422","article-title":"Comparison of virus replication efficiency in lymphoid tissue among three infectious bursal disease virus strains","volume":"39","author":"Tsukamoto K.","year":"1995","journal-title":"Avian Dis."},{"key":"17","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0165-2427(02)00206-4","article-title":"Protective immunity against infectious bursal disease virus in chickens in the absence of virus-specific antibodies","volume":"89","author":"Yeh H. 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