{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:34:27Z","timestamp":1754397267738},"reference-count":21,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>The incidence of outbreaks of foodborne listeriosis has indicated the need for a reliable and rapid detection of the microbe in different foodstuffs. A method combining redox potential measurement and real-time polymerase chain reaction (PCR) was developed to detect <jats:italic>Listeria monocytogenes<\/jats:italic> in artificially contaminated raw milk and soft cheese. Food samples of 25 g or 25 ml were homogenised in 225 ml of Listeria Enrichment Broth (LEB) with Oxford supplement, and the redox potential measurement technique was applied. For <jats:italic>Listeria<\/jats:italic> species the measuring time was maximum 34 h. The absence of <jats:italic>L. monocytogenes<\/jats:italic> could reliably be proven by the redox potential measurement method, but <jats:italic>Listeria innocua<\/jats:italic> and <jats:italic>Bacillus subtilis<\/jats:italic> could not be differentiated from <jats:italic>L. monocytogenes<\/jats:italic> on the basis of the redox curves. The presence of <jats:italic>L. monocytogenes<\/jats:italic> had to be confirmed by real-time PCR. The combination of these two methods proved to detect &lt; 10 cfu\/g of <jats:italic>L. monocytogenes<\/jats:italic> in a cost- and time-effective manner. This method can potentially be used as an alternative to the standard nutrient method for the rapid detection of <jats:italic>L. monocytogenes<\/jats:italic> in food.<\/jats:p>","DOI":"10.1556\/avet.2014.013","type":"journal-article","created":{"date-parts":[[2014,7,20]],"date-time":"2014-07-20T01:00:58Z","timestamp":1405818058000},"page":"304-316","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":4,"title":["Rapid detection of Listeria monocytogenes in raw milk and soft cheese by a redox potential measurement based method combined with real-time PCR"],"prefix":"10.1556","volume":"62","author":[{"given":"Orsolya","family":"Erd\u0151si","sequence":"first","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Szakm\u00e1r","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliv\u00e9r","family":"Reichart","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Szili","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"No\u00e9mi","family":"L\u00e1szl\u00f3","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9ter","family":"Sz\u00e9kely K\u00f6rm\u00f6czy","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9ter","family":"Laczay","sequence":"additional","affiliation":[{"name":"1 Szent Istv\u00e1n University Department of Food Hygiene, Faculty of Veterinary Science Istv\u00e1n u. 2 H-1078 Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"6273","DOI":"10.1128\/AEM.68.12.6273-6282.2002","article-title":"Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods","volume":"68","author":"Cocolin L.","year":"2002","journal-title":"App. Environ. 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