{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:56:47Z","timestamp":1757314607543},"reference-count":23,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,7,1]]},"abstract":"<jats:p>The extenders and freezing rates from three different freezing protocols were combined and compared to each other in order to study the post-thawing acrosome integrity and fertility of frozen dog sperm. A commercial bovine TRIS-base extender (TRILADYL) and two self-made canine semen extenders (Norwegian and Dutch) were combined with a conventional bovine and two canine freezing regimes, and acrosome integrity of frozen\/thawed spermatozoa was assessed by fluorescein isothiocyanate conjugated peanut agglutinin staining (FITC-PNA). Differences between freezing\/thawing protocols were reflected in the proportion of cells with acrosomal damage and not based on motility results. It was concluded that during dog semen cryopreservation extenders had less influence on the post-thawing sperm quality than did the freezing rates. The optimal extender\/freezing rate combination (TRILADYL\/Norwegian) was used in the clinical practice to evaluate the fertility of frozen sperm administered by intrauterine insemination using a surgical approach. The pregnancy rate was 57% (4\/7), but the average litter size was low (2.8). This may have been due to the insufficient sperm numbers contained in an insemination dose and\/or to the incorrect timing of artificial insemination (AI). The final conclusion is that the commercial bovine extender is useful for freezing dog semen, and the TRILADYL\/Norwegian freezing protocol is recommended as the most advantageous combination for the freezing of canine semen in the clinical practice.<\/jats:p>","DOI":"10.1556\/avet.48.2000.3.9","type":"journal-article","created":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T19:28:11Z","timestamp":1129922891000},"page":"325-333","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":7,"title":["COMPARATIVE STUDY OF DIFFERENT METHODS FOR DOG SEMEN CRYOPRESERVATION AND TESTING UNDER CLINICAL CONDITIONS"],"prefix":"10.1556","volume":"48","author":[{"given":"F.","family":"Sz\u00e1sz","sequence":"first","affiliation":[{"name":"1 Department of Obstetrics and Reproduction, Faculty of Veterinary Science, Szent Istv\u00e1n University Please ask the editor of the journal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"G\u00e1bor","sequence":"additional","affiliation":[{"name":"2 Department of Obstetrics and Reproduction, Faculty of Veterinary Science, Szent Istv\u00e1n University Please ask the editor of the journal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Solti","sequence":"additional","affiliation":[{"name":"3 Department and Clinic of Obstetrics and Animal Reproduction, Faculty of Veterinary Science, Szent Istv\u00e1n University Department of Obstetrics and Reproduction Istv\u00e1n u. 2. 1078 Budapest"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"8","first-page":"48","article-title":"Artificial insemination with fresh, chilled extended, and frozen-thawed semen in the dog.","volume":"10","author":"C. Linde-Forsberg","year":"1995","journal-title":"Sem. Vet. Med. Surg."},{"key":"23","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/j.1748-5827.1987.tb01293.x","article-title":"Some observations on the dilution, cooling and freezing of canine semen.","volume":"28","author":"C. Yubi","year":"1987","journal-title":"J. Small Anim. Pract."},{"key":"4","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1748-5827.1984.tb03429.x","article-title":"Bitch fertility after natural mating and artificial insemination with fresh or frozen semen.","volume":"25","author":"W. Farstad","year":"1984","journal-title":"J. Small Anim. Pract."},{"key":"1","first-page":"229","volume-title":"Canine sperm post-thaw survival following freezing in straws or pellets using PIPES, Lactose, TRIS or TEST extenders","volume":"3","author":"M. Battista","year":"1988"},{"key":"14","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1095\/biolreprod38.5.1171","article-title":"Capacitation of bovine sperm by heparin.","volume":"38","author":"J. Parrish","year":"1988","journal-title":"Biol. Reprod."},{"key":"15","first-page":"1013","article-title":"Successful pregnancies with concentrated frozen canine semen.","volume":"27","author":"C. Platz","year":"1977","journal-title":"Lab. Anim. Sci."},{"key":"16","first-page":"279","article-title":"Fine structure and elemental composition of fresh and frozen dog spermatozoa.","volume":"47","author":"H. Rodriguez-Martinez","year":"1993","journal-title":"J. Reprod. Fert. Suppl."},{"key":"17","first-page":"6","article-title":"Successful pregnancies utilizing frozen dog semen.","volume":"17","author":"S. Seager","year":"1969","journal-title":"AI Digest"},{"key":"18","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1530\/jrf.0.0450189","article-title":"Conception rates and related data using frozen dog semen.","volume":"45","author":"S. Seager","year":"1975","journal-title":"J. Reprod. Fert."},{"key":"19","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/0093-691X(95)00228-Z","article-title":"Comparisons between three different extenders for canine intrauterine insemination with frozen-thawed spermatozoa.","volume":"44","author":"L. Silva","year":"1995","journal-title":"Theriogenology"},{"key":"20","first-page":"177","article-title":"Induction of acrosome reaction in dog sperm by calcium ionophore.","volume":"45","author":"F. Sz\u00e1sz","year":"1997","journal-title":"Acta Vet. Hung."},{"key":"21","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1292\/jvms1939.51.257","article-title":"Relationship between the number of sperms and the rate of implantation in bitches inseminated into unilateral uterine horn.","volume":"51","author":"T. Tsutsui","year":"1989","journal-title":"Jpn. J. Vet. Sci."},{"key":"22","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/978-1-4684-4016-4_6","volume-title":"Fertilization and Embryonic Development In Vitro","author":"R. Yanagimachi","year":"1981"},{"key":"2","first-page":"291","article-title":"Effects of four different extenders and three different freezing rates on post-thaw viability of dog semen.","volume":"47","author":"I. Dobrinski","year":"1993","journal-title":"J. Repord. Fert. Suppl."},{"key":"3","volume-title":"The Cryopreservation of Dog Semen","author":"G. England","year":"1992"},{"key":"5","first-page":"289","article-title":"Factors influencing the success rate of artificial insemination with frozen semen in the dog.","volume":"39","author":"W. Farstad","year":"1989","journal-title":"J. Reprod. Fert. Suppl."},{"key":"6","first-page":"325","article-title":"Canine artificial insemination with frozen semen: Comparison of intravaginal and intrauterine deposition of semen.","volume":"47","author":"A. Fontbonne","year":"1993","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"7","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/0093-691X(95)00227-Y","article-title":"Effect of thawing regimens on the morphofunctional state of canine spermatozoa.","volume":"44","author":"M. Ivanova-Kicheva","year":"1995","journal-title":"Theriogenology"},{"key":"9","first-page":"91","volume-title":"Basic concepts of freezing cells","author":"P. Mazur","year":"1985"},{"key":"10","volume-title":"Praktikum der Hundeklinik","author":"H. Niemand","year":"1994"},{"key":"11","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0378-4320(86)90054-0","article-title":"Changes in acrosome morphology during cooling and freezing of dog semen.","volume":"12","author":"E. Oettl\u00e9","year":"1986","journal-title":"Anim. Repord. Sci."},{"key":"12","volume-title":"Cryopreservation of dog spermatozoa","author":"T. Olar","year":"1984"},{"key":"13","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0093-691X(89)90550-5","article-title":"Influence of extender, cryopreservative and seminal processing procedures on post thaw motility of canine spermatozoa frozen in straws.","volume":"31","author":"T. Olar","year":"1989","journal-title":"Theriogenology"}],"container-title":["Acta Veterinaria Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/48\/3\/article-p325.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/48\/3\/article-p325.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T18:31:01Z","timestamp":1612031461000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/48\/3\/article-p325.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,7,1]]},"references-count":23,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/avet.48.2000.3.9","relation":{},"ISSN":["0236-6290","1588-2705"],"issn-type":[{"value":"0236-6290","type":"print"},{"value":"1588-2705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,7,1]]}}}