{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:35:08Z","timestamp":1759941308259},"reference-count":25,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"The relationship between concentration of calcium ionophore A23187 and incubation time upon the proportion of spermatozoa undergoing acrosome reaction (AR) in vitro<\/jats:italic> was investigated in rams from a commercial artificial insemination (AI) program. Two ejaculates were collected by artificial vagina from each of nine rams of three breeds (Finn Dorset, Charolais and Suffolk) aged 8-36months. Each ejaculate was diluted in a skimmed milk extender. Spermatozoa were thereafter incubated for 45 or 60min in modified Tyrode's medium (TALP) which contained either zero, 0.1 or 1.0\u00b5M\/l A23187. After fixing in 10% formaldehyde, the number of spermatozoa that had undergone AR was determined by phase contrast microscopy. In pre-incubation samples, 21.3\u00b1 3.3% of spermatozoa had undergone AR. Percentages of acrosome reacted spermatozoa were significantly (P<0.001) increased after incubation with A23187. After incubation with 0.1\u00b5M\/l A23187 for 45 and 60min there were 22.4\u00b13.0% and 31.7\u00b14.3% acrosome reacted spermatozoa, respectively. After incubation with 1.0\u00b5M\/l A23187 for 45 and 60min there were 46.2\u00b16.5% and 53.8\u00b15.9% acrosome reacted spermatozoa, whilst corresponding numbers in control samples were 17.0\u00b12.7% and 22.3\u00b14.2%. There was also a significant (P<0.001) effect of individual animals upon the responses to different concentrations of A23187. These findings indicate that (i) A23187 can be used to assess the AR of ovine spermatozoa in vitro <\/jats:italic>and (ii) there are effects of individual animals upon the proportion of spermatozoa undergoing AR.<\/jats:p>","DOI":"10.1556\/avet.51.2003.1.10","type":"journal-article","created":{"date-parts":[[2004,7,14]],"date-time":"2004-07-14T13:51:59Z","timestamp":1089813119000},"page":"103-109","source":"Crossref","is-referenced-by-count":3,"title":["In vitro induction of the acrosome reaction in ovine spermatozoa by calcium ionophore A23187"],"prefix":"10.1556","volume":"51","author":[{"given":"\u00d6.","family":"U\u00e7ar","sequence":"first","affiliation":[{"name":"1 Department of Clinical Veterinary Sciences, Bristol University Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Parkinson","sequence":"additional","affiliation":[{"name":"2 Department of Clinical Veterinary Sciences, Bristol University Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"7","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/0093-691X(94)90382-S","article-title":"The use of bright-field microscopy in evaluating bovine acrosome reaction.","volume":"42","author":"P. Christensen","year":"1994","journal-title":"Theriogenology"},{"key":"8","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1095\/biolreprod41.4.635","article-title":"Methods for evaluating the acrosomal status of mammalian sperm.","volume":"41","author":"N. Cross","year":"1989","journal-title":"Biol. Reprod."},{"key":"9","first-page":"107","volume-title":"Dilution of semen","author":"G. 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Reprod."},{"key":"11","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1071\/RD9960581","article-title":"Capacitation mechanisms, and the role of capacitation as seen in eutherian mammals.","volume":"8","author":"R. P. Harrison","year":"1996","journal-title":"Reprod. Fertil. Dev."},{"key":"17","first-page":"193","article-title":"Effect of handling on viability and acrosome status of ram spermatozoa.","volume":"37","author":"F. Sarhaddi","year":"1995","journal-title":"Acta Agronomica \u00f3variensis"},{"key":"18","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/mrd.1120040506","article-title":"Production, characterisation, and use of ionophore- induced, calcium-dependent acrosome reaction in ram spermatozoa.","volume":"4","author":"G. Shams-Borhan","year":"1981","journal-title":"Gamete Res."},{"key":"19","first-page":"223","volume-title":"Statistical Methods","author":"G. 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