{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T09:20:30Z","timestamp":1722936030609},"reference-count":38,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"<jats:p>Besides the well-known O157:H7 clone causing enterohaemorrhagic colitis and haemolytic uraemic syndrome in Europe, Japan and North America, the number of <jats:italic>Escherichia coli<\/jats:italic> isolates with non-motile (NM) phenotype has considerably increased. We supposed that spontaneous antibiotic resistance mutation could cause this phenotypic change. To model our hypothesis we isolated rifampicin- (Rif) and ampicillin- (Amp) resistant mutants from <jats:italic>E. coli<\/jats:italic> O157:H7 prototype strains 7785 and EDL933. Among Rif<jats:sup> r<\/jats:sup> mutants we could isolate strains with no or reduced motility, while the Amp<jats:sup>r<\/jats:sup> mutants became hypermotile. The biochemical profile of the mutants had not changed but phage sensitivity and generation time of the mutants were altered. Among the representative strains we did not find polymorphism with Southern blot analysis and no polymorphism was found in the <jats:italic>fliC<\/jats:italic> gene of the mutants. The described characteristics have proven to be stable. In a mice virulence assay by intravenous infections the virulence of the derivatives was also found to be changed. In summary, we found that the antibiotic-resistant phenotype in <jats:italic>E. coli<\/jats:italic> O157:H7 was coexpressed with several other phenotypic changes including motility and virulence. It can be assumed that expression of the involved phenotypes may be under the influence of a common regulatory cascade. Further work is needed to identify the components and mechanism of this regulatory system.<\/jats:p>","DOI":"10.1556\/avet.51.2003.1.3","type":"journal-article","created":{"date-parts":[[2004,7,14]],"date-time":"2004-07-14T13:51:59Z","timestamp":1089813119000},"page":"29-44","source":"Crossref","is-referenced-by-count":8,"title":["Spontaneous antibiotic resistance mutation associated pleiotropic changes in Escherichia coli O157:H7"],"prefix":"10.1556","volume":"51","author":[{"given":"I.","family":"T\u00f3th","sequence":"first","affiliation":[{"name":"1 Department of General and Thoracic Surgery, Semmelweis Teaching Hospital H-3529 Miskolc, Csabai kapu 9-11, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rta","family":"Cs\u00edk","sequence":"additional","affiliation":[{"name":"2 J\u00f3zsef Fodor' National Center for Public Health Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Em\u00e7dy","sequence":"additional","affiliation":[{"name":"3 Department of Medical Microbiology and Immunology, University of P\u00e9cs P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"27","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/S0140-6736(83)91987-6","article-title":"Escherichia coli O157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga)-like cytotoxin.","volume":"1","author":"A. 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