{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:08:48Z","timestamp":1791058128726,"version":"4.1.0"},"reference-count":38,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:p>Canine Lyme borreliosis may be caused by three <jats:italic>Borrelia burgdorferi<\/jats:italic> sensu lato genospecies. The prevalence of infection by <jats:italic>Borrelia<\/jats:italic> species was determined by nested polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with the enzyme <jats:italic>Fsp4H<\/jats:italic> I in the blood of dogs naturally infested by ticks in an endemic region of Poland. Blood samples were collected from 98 dogs of various breeds, delivered to the Veterinary Clinic in Szczecin (northwestern Poland) for various reasons. Nested PCR revealed the presence of DNA characteristic of only 1 genospecies, i.e. <jats:italic>B. burgdorferi<\/jats:italic> sensu stricto (s.s.), in all PCR-positive samples. Digestion of PCR products from a fragment of the <jats:italic>fla<\/jats:italic> gene amplified with primers FLA1 and FLA2 gave only one band pattern consistent with the pattern obtained from sequence analysis of the <jats:italic>fla<\/jats:italic> gene from a reference isolate of <jats:italic>B. burgdorferi<\/jats:italic> s.s. GeHo (X15660) from GenBank.<\/jats:p>","DOI":"10.1556\/avet.53.2005.1.2","type":"journal-article","created":{"date-parts":[[2005,5,23]],"date-time":"2005-05-23T12:31:58Z","timestamp":1116851518000},"page":"13-21","source":"Crossref","is-referenced-by-count":13,"title":["Identification of Borrelia burgdorferi genospecies inducing Lyme disease in dogs from Western Poland"],"prefix":"10.1556","volume":"53","author":[{"given":"Bogumi\u0142a","family":"Skotarczak","sequence":"first","affiliation":[{"name":"1 Department of Genetics, Faculty of Biology, Szczecin University 71-215 Szczecin, Piastow 40B, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Wodecka","sequence":"additional","affiliation":[{"name":"2 Department of Genetics, Faculty of Biology, Szczecin University 71-215 Szczecin, Piastow 40B, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"8","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/BF01719437","article-title":"Seroepidemiological survey for Borrelia burgdorferi (Lyme disease) in dogs from northwestern of Spain","volume":"11","author":"S. 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