{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:42:51Z","timestamp":1792680171659,"version":"4.5.0"},"reference-count":14,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:p>Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst\/FITC-<jats:italic>Pisum sativum<\/jats:italic>agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-PSA labelling (39.33%). The viability of sperm stained by the double staining method was 67.17%, and a higher portion of viable sperm (82.67%) was observed by staining with the Hoechst procedure (P &lt; 0.01). On the basis of the results obtained it is concluded that both methods can be used for detecting the acrosome reaction of frozen-thawed bovine spermatozoa.<\/jats:p>","DOI":"10.1556\/avet.56.2008.1.14","type":"journal-article","created":{"date-parts":[[2008,4,12]],"date-time":"2008-04-12T00:15:47Z","timestamp":1207959347000},"page":"133-138","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":14,"title":["Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods"],"prefix":"10.1556","volume":"56","author":[{"given":"Jana","family":"Jankovi\u010dov\u00e1","sequence":"first","affiliation":[{"name":"1 Slovak Academy of Sciences Institute of Animal Biochemistry and Genetics Moyzesova 61 900 28 Ivanka pri Dunaji Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Simon","sequence":"additional","affiliation":[{"name":"1 Slovak Academy of Sciences Institute of Animal Biochemistry and Genetics Moyzesova 61 900 28 Ivanka pri Dunaji Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Antal\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"1 Slovak Academy of Sciences Institute of Animal Biochemistry and Genetics Moyzesova 61 900 28 Ivanka pri Dunaji Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u013dubica","family":"Horovsk\u00e1","sequence":"additional","affiliation":[{"name":"1 Slovak Academy of Sciences Institute of Animal Biochemistry and Genetics Moyzesova 61 900 28 Ivanka pri Dunaji Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1093\/oxfordjournals.humrep.a137910","article-title":"Analysis of the ability of three probes targeting the outer acrosomal membrane or acrosomal contents to detect the acrosome reaction in human spermatozoa","volume":"8","author":"Aitken R. 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