{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T02:16:01Z","timestamp":1782958561886,"version":"3.54.5"},"reference-count":23,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>Studies were performed on boar semen routinely used at the local artificial insemination (AI) centre. The semen was stored in a Safe Cell Plus commercial extender at 17 \u00b0C for nine days. The aim of our research was focused on changes in sperm plasma membrane integrity. The integrity of the sperm plasma membrane and acrosome as well as sperm motility decreased after dilution and during storage of the semen. The highest percentage of live sperm was identified by the eosin-nigrosin method, a lower percentage by the SYBR-14\/PI test, and the lowest percentage of live cells was discovered by the hypoosmotic swelling (HOS) test (P &lt; 0.01). There were significant differences between the results of staining methods and sperm motility (P &lt; 0.01). No significant differences were found between the HOS test results and sperm motility. The plasma membrane integrity parameters positively correlated (P &lt; 0.001) with each other and with sperm motility but negatively with aspartate aminotransferase activity. Our findings confirmed that the boar sperm aging changes, which increased during liquid semen preservation, were connected with the loss of function and integrity of the sperm plasma membrane. The employed complementary tests are comprehensive indicators of sperm membrane integrity during long-term semen preservation, and they can help establish the actual number of \u2018healthy\u2019 cells. The assays may be used in AI laboratories and should be incorporated into the routine of semen analysis.<\/jats:p>","DOI":"10.1556\/avet.58.2010.1.11","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T02:02:28Z","timestamp":1266372148000},"page":"105-116","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":12,"title":["Plasma membrane changes during the liquid storage of boar spermatozoa: A comparison of methods"],"prefix":"10.1556","volume":"58","author":[{"given":"Dariusz","family":"G\u0105czarzewicz","sequence":"first","affiliation":[{"name":"1 West Pomeranian University of Technology Department of Animal Reproduction Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding Doktora Judyma 6 71466 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Piasecka","sequence":"additional","affiliation":[{"name":"2 Pomeranian Medical University Laboratory of Histology and Developmental Biology \u017bo\u0142nierska 48 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Uda\u0142a","sequence":"additional","affiliation":[{"name":"1 West Pomeranian University of Technology Department of Animal Reproduction Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding Doktora Judyma 6 71466 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"B\u0142aszczyk","sequence":"additional","affiliation":[{"name":"1 West Pomeranian University of Technology Department of Animal Reproduction Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding Doktora Judyma 6 71466 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Stankiewicz","sequence":"additional","affiliation":[{"name":"1 West Pomeranian University of Technology Department of Animal Reproduction Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding Doktora Judyma 6 71466 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Laszczy\u0144ska","sequence":"additional","affiliation":[{"name":"2 Pomeranian Medical University Laboratory of Histology and Developmental Biology \u017bo\u0142nierska 48 Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"1","volume-title":"Animal Reproduction (in Polish)","author":"Biela\u0144ski W.","year":"1977"},{"key":"2","first-page":"239","article-title":"Morphological evaluation of bovine spermatozoa. II. Proposition of new classification of sperm defects (in Polish)","volume":"37","author":"Blom E.","year":"1981","journal-title":"Medycyna Wet."},{"key":"3","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0093-691X(03)00174-2","article-title":"Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate","volume":"60","author":"Brito L. F.","year":"2003","journal-title":"Theriogenology"},{"key":"4","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0378-4320(99)00035-4","article-title":"Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage","volume":"58","author":"Cerolini S.","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"5","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0378-4320(94)90028-0","article-title":"Stimulation of aspartate aminotransferase from farm animal semen by pyridoxal 5\u2032-phosphate","volume":"34","author":"Ciereszko A.","year":"1994","journal-title":"Anim. Reprod. Sci."},{"key":"6","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1111\/j.1365-2605.2006.00694.x","article-title":"Effect of storage in short- and long-term com commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa","volume":"29","author":"Ambrogi M.","year":"2006","journal-title":"Int. J. Androl."},{"key":"7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1046\/j.1439-0531.2001.00310.x","article-title":"Relationship between motility and membrane integrity of boar spermatozoa in media varying in osmolality","volume":"36","author":"Fraser L.","year":"2001","journal-title":"Reprod. Dom. Anim."},{"key":"8","doi-asserted-by":"crossref","first-page":"17","DOI":"10.5424\/sjar\/2003012-17","article-title":"Semen extenders used in the artificial insemination of swine. A review","volume":"1","author":"Gadea J.","year":"2003","journal-title":"Spanish J. Agric. Res."},{"key":"9","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0378-4320(00)00157-3","article-title":"Storage of boar semen","volume":"62","author":"Johnson L. A.","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/1751-0147-43-49","article-title":"Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days","volume":"43","author":"Kommisrud E.","year":"2002","journal-title":"Acta Vet. Scand."},{"key":"11","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1046\/j.1439-0272.2000.00351.x","article-title":"Assessing equine sperm-membrane integrity","volume":"32","author":"Lagares M. A.","year":"2000","journal-title":"Andrologia"},{"key":"12","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1046\/j.1439-0531.2002.t01-1-00381.x","article-title":"The use of HOS test to evaluate membrane functionality of boar sperm capacitated in vitro","volume":"37","author":"Lechniak D.","year":"2002","journal-title":"Reprod. Dom. Anim."},{"key":"13","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/j.1439-0531.2005.00599.x","article-title":"Variations in the proportion of glycolytic\/non-glycolytic energy substrates modulate sperm membrane integrity and function in diluted boar samples stored at 15\u201317 \u00b0C","volume":"40","author":"Medrano A.","year":"2005","journal-title":"Reprod. Dom. Anim."},{"key":"14","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/S0093-691X(00)00428-3","article-title":"Assessment of stallion spermatozoa viability by flow cytometry and light microscope analysis","volume":"54","author":"Merkies K.","year":"2000","journal-title":"Theriogenology"},{"key":"15","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/S0093-691X(99)00207-1","article-title":"Evaluation of sperm tail membrane integrity by light microscopy","volume":"52","author":"Nagy Sz.","year":"1999","journal-title":"Theriogenology"},{"key":"16","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1365-2605.2005.00512.x","article-title":"A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa","volume":"28","author":"Pe\u0148a F. J.","year":"2005","journal-title":"Int. J. Androl."},{"key":"17","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1016\/j.theriogenology.2006.01.066","article-title":"Sperm DNA fragmentation in boars is delayed or abolished by using sperm extenders","volume":"66","author":"P\u00e9rez-Llano B.","year":"2006","journal-title":"Theriogenology"},{"key":"18","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1439-0531.2006.00677.x","article-title":"Effects of constant, 9- and 16-h light cycles on sperm quality, semen storage ability and motile sperm subpopulations structure of boar semen","volume":"41","author":"Rivera M. M.","year":"2006","journal-title":"Reprod. Dom. Anim."},{"key":"19","unstructured":"Saake, R. G. and White, M. (1972): Semen quality tests and their relationship to fertility. Proc. Fourth Techn. Conf. Anim. Reprod. Artif. Insem., 18\u201320 April 1972, Madison, WI, National Association of Animal Breeders, Columbia, MO, USA. pp. 22\u201327."},{"key":"20","first-page":"278","article-title":"Comparison of in vitro methods for evaluation of boar semen quality","volume":"61","author":"\u0160ernien\u0117 L.","year":"2005","journal-title":"Medycyna Wet"},{"key":"21","first-page":"243","article-title":"Secretory activity of boar seminal vesicle glands","volume":"2","author":"Strze\u017cek J.","year":"2002","journal-title":"Reprod. Biol."},{"key":"22","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S0093-691X(97)00046-0","article-title":"Hypoosmotic swelling of boar spermatozoa compared to other methods for analysing the sperm membrane","volume":"47","author":"Vazquez J. M.","year":"1997","journal-title":"Theriogenology"},{"key":"23","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1046\/j.1439-0531.2003.00468.x","article-title":"Comparative study on five different commercial extenders for boar semen","volume":"39","author":"Vyt P.","year":"2004","journal-title":"Reprod. Dom. Anim."}],"container-title":["Acta Veterinaria Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/58\/1\/article-p105.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/58\/1\/article-p105.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T18:28:25Z","timestamp":1612031305000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/58\/1\/article-p105.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,1]]},"references-count":23,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/avet.58.2010.1.11","relation":{},"ISSN":["0236-6290","1588-2705"],"issn-type":[{"value":"0236-6290","type":"print"},{"value":"1588-2705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,1]]}}}