{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T01:33:31Z","timestamp":1769045611104,"version":"3.49.0"},"reference-count":22,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>The aim of the present study was to detect and characterise the species and subspecies of \n <jats:italic>Babesia<\/jats:italic>\n spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of \n <jats:italic>Babesia canis<\/jats:italic>\n . The results showed the detection of one subspecies, namely \n <jats:italic>Babesia canis canis<\/jats:italic>\n using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with \n <jats:italic>B. canis canis<\/jats:italic>\n . The results of this study confirm the presence of \n <jats:italic>B. canis canis<\/jats:italic>\n in infected dogs in Croatia and demonstrate a slightly new genetic variant of \n <jats:italic>Babesia<\/jats:italic>\n subspecies.<\/jats:p>","DOI":"10.1556\/avet.58.2010.1.4","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T02:02:28Z","timestamp":1266372148000},"page":"39-46","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":12,"title":["Molecular evidence of natural infection with Babesia canis canis in Croatia"],"prefix":"10.1556","volume":"58","author":[{"given":"Mirna","family":"Brklja\u010di\u0107","sequence":"first","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"Matijatko","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Ki\u0161","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nada","family":"Ku\u010der","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jadranka","family":"For\u0161ek","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Rafaj","sequence":"additional","affiliation":[{"name":"2 University of Zagreb Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darko","family":"Grden","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marin","family":"Torti","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Mayer","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Mrljak","sequence":"additional","affiliation":[{"name":"1 University of Zagreb Clinic for Internal Diseases Heinzelova 55 10 000 Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.4269\/ajtmh.1998.58.739","article-title":"Diversity of Babesia infecting deer ticks (Ixodes dammini)","volume":"58","author":"Armstrong P. 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