{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:46:49Z","timestamp":1775742409391,"version":"3.50.1"},"reference-count":16,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>An outbreak of simultaneously occurring haemangiomas, leiomyosarcoma and myeloma was observed in a commercial layer flock in China. The sick chickens were extremely thin and dehydrated. Scattered haemangiomas were found on the claws, breast and wings. At necropsy, haemangiomas and some other nodular tumours were also found in the internal organs. In addition, diffuse enlargement of the liver and spleen appeared in some birds. Histopathologically, haemangiomas were typically cavernous haemangiomas and haemangioendothelioma. In the diffusely swollen liver and spleen, multifocal or widespread marrow tumour cells filled with ball-like acidophilic particles in cytosol were observed, which are the characteristic pathological changes of avian myelocytomatosis. The nodular tumour cells formed by muscle bundles were of variable size, irregular shape, poorly differentiated and malaligned. Immunohistochemistry for vimentin, cytokeratin, actin (smooth muscle) and actin (sarcomeric) and Masson\u2019s staining confirmed the different cell lineage of the nodular tumour, thus leading to the diagnosis of leiomyosarcoma. The seroprevalence of avian leukosis subgroup J (ALV-J) antibodies was 13.46% (7\/52), while ALV-A\/B and reticuloendotheliosis virus (REV) antibodies were not detectable. The DF-1 cells inoculated by virus extracted from liver samples from 24 infected chickens were cultured and the group-specific antigen (GSA) was identified by ELISA. All samples were positive for ALV, which was further identified as ALV-J by immunofluorescence assay (IFA). PCR analysis revealed that three isolates of ALV-J proviral sequence were close to the HPRS-103 prototype strain and other Chinese field strains isolated in recent years, while one isolate (DP01) had a lower homology with them. This is the first report that ALV-J infection caused the simultaneous occurrence of haemangiomas, leiomyosarcoma and myeloma in a commercial layer flock.<\/jats:p>","DOI":"10.1556\/avet.58.2010.4.5","type":"journal-article","created":{"date-parts":[[2010,11,19]],"date-time":"2010-11-19T02:21:07Z","timestamp":1290133267000},"page":"441-451","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":19,"title":["Haemangiomas, leiomyosarcoma and myeloma caused by subgroup J avian leukosis virus in a commercial layer flock"],"prefix":"10.1556","volume":"58","author":[{"given":"Honglei","family":"Sun","sequence":"first","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Qin","sequence":"additional","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Xiao","sequence":"additional","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Yang","sequence":"additional","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Ni","sequence":"additional","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidang","family":"Liu","sequence":"additional","affiliation":[{"name":"1 Shandong Agricultural University College of Animal Science and Veterinary Medicine Taian 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.2307\/1592351","article-title":"A low incidence of histiocytic sarcomatosis associated with infection of chickens with HPRS-103 strain of subgroup J avian leucosis virus","volume":"41","author":"Arshad S. S.","year":"1997","journal-title":"Avian Dis."},{"key":"2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1177\/030098589703400205","article-title":"Tissue tropism of the HPRS-103 strain of J subgroup avian leukosis virus and a derivative acutely transforming virus","volume":"34","author":"Arshad S. S.","year":"1997","journal-title":"Vet. Pathol."},{"key":"3","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1080\/03079459994894","article-title":"Tropism of subgroup J avian leukosis virus as detected by in situ hybridization","volume":"28","author":"Arshad S. S.","year":"1999","journal-title":"Avian Pathol"},{"key":"4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/03079450310001636237a","article-title":"Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China","volume":"33","author":"Binrui X.","year":"2004","journal-title":"Avian Pathol."},{"key":"5","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1099\/0022-1317-83-10-2553","article-title":"The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J, respectively","volume":"83","author":"Chesters P. M.","year":"2002","journal-title":"J. Gen. Virol."},{"key":"6","first-page":"341","article-title":"Isolation of subgroup J avian leukosis viruses and their partial sequence comparison","volume":"16","author":"Du Y.","year":"2000","journal-title":"Chinese J. Virol."},{"key":"7","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2307\/1592636","article-title":"Isolation and some characteristics of a subgroup J-like avian leukosis virus associated with myeloid leukosis in meat-type chickens in the United States","volume":"43","author":"Fadly A. M.","year":"1999","journal-title":"Avian Dis."},{"key":"8","first-page":"82","article-title":"Hemangioblastoma: an immunohistochemical study of ten cases","volume":"76","author":"Grant J. W.","year":"1998","journal-title":"Acta Neuropathol."},{"key":"9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1080\/03079459808419291","article-title":"HPRS-103: a retrovirus strikes back. The emergence of subgroup J avian leukosis virus","volume":"27","author":"Payne L. N.","year":"1998","journal-title":"Avian Pathol."},{"key":"10","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1099\/0022-1317-72-4-801","article-title":"A novel subgroup of exogenous avian leukosis virus in chickens","volume":"72","author":"Payne L. N.","year":"1991","journal-title":"J. Gen. Virol."},{"key":"11","first-page":"1167","article-title":"Myeloid leukaemogenicity and transmission of the HPRS-103 strain in avian leukosis virus","volume":"6","author":"Payne L. N.","year":"1992","journal-title":"Leukemia"},{"key":"12","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1006\/viro.2000.0352","article-title":"Hypervariability in the envelope genes of subgroup J avian leukosis viruses obtained from different farms in the United States","volume":"272","author":"Silva R. F.","year":"2000","journal-title":"Virology"},{"key":"13","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1080\/0307945042000220435","article-title":"Detection of avian leukosis virus subgroup J in chicken flocks from Malaysia and their molecular characterization","volume":"33","author":"Thapa B. R.","year":"2004","journal-title":"Avian Pathol."},{"key":"14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1053\/rvsc.1998.0283","article-title":"Avian leukosis virus subgroup J: a rapidly evolving group of oncogenic retroviruses","volume":"67","author":"Venugopal K.","year":"1999","journal-title":"Res. Vet. Sci."},{"key":"15","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1080\/03079450120092134","article-title":"Reduction of horizontal transmission of avian leukosis virus subgroup J in broiler chickens hatched and reared in small groups","volume":"30","author":"Witter R. L.","year":"2001","journal-title":"Avian Pathol."},{"key":"16","first-page":"39","article-title":"Hemangioma and myelocytomas induced by avian leukosis virus subgroup J in commercial layer flocks","volume":"64","author":"Zhang H.","year":"2009","journal-title":"Isr. J. Vet. Med."}],"container-title":["Acta Veterinaria Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/58\/4\/article-p441.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/58\/4\/article-p441.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T18:28:06Z","timestamp":1612031286000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/58\/4\/article-p441.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,1]]},"references-count":16,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/avet.58.2010.4.5","relation":{},"ISSN":["0236-6290","1588-2705"],"issn-type":[{"value":"0236-6290","type":"print"},{"value":"1588-2705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,1]]}}}