{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T17:27:02Z","timestamp":1750181222204},"reference-count":12,"publisher":"Akademiai Kiado Zrt.","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3,1]]},"abstract":"<jats:p>\n          <jats:italic>Bevezet\u00e9s:<\/jats:italic>\n Az anyai v\u00e9rben kering\u0151 szabad magzati DNS kimutat\u00e1sa a noninvaz\u00edv praenatalis diagnosztika egyik sarokk\u00f6v\u00e9v\u00e9 v\u00e1lt az elm\u00falt k\u00e9t \u00e9vtized sor\u00e1n. Az anya perif\u00e9ri\u00e1s kering\u00e9s\u00e9ben fellelhet\u0151 szabad magzati DNS felhaszn\u00e1l\u00e1sa a praenatalis diagnosztik\u00e1ban az anyai \u00e9s magzati eredet\u0171 DNS k\u00fcl\u00f6nbs\u00e9g\u00e9n alapszik. Az egyik ilyen k\u00fcl\u00f6nbs\u00e9g az anya \u00e9s a magzat k\u00f6z\u00f6tti v\u00e9rcsoportelt\u00e9r\u00e9s genetikai h\u00e1ttere. A v\u00e9rcsoport-\u00f6sszef\u00e9rhetetlens\u00e9gek k\u00f6z\u00fcl az Rh-konstell\u00e1ci\u00f3 a leggyakoribb a klinikai gyakorlatban. \n <jats:italic>C\u00e9lkit\u0171z\u00e9s:<\/jats:italic>\n A szerz\u0151k \u00e1ltal v\u00e9gzett vizsg\u00e1lat c\u00e9lja, a szakirodalomban fellelhet\u0151 eredm\u00e9nyek t\u00fckr\u00e9ben, a magzati Rh-st\u00e1tus anyai v\u00e9rb\u0151l t\u00f6rt\u00e9n\u0151 meghat\u00e1roz\u00e1sa volt. \n <jats:italic>M\u00f3dszer:<\/jats:italic>\n A terhess\u00e9g 11. \u00e9s 22. hete k\u00f6z\u00f6tt 30 Rh-negat\u00edv v\u00e9rcsoport\u00fa terhes v\u00e9rplazm\u00e1j\u00e1b\u00f3l, valamint a v\u00e9rv\u00e9telt k\u00f6vet\u0151 amniocent\u00e9zis sor\u00e1n nyert magzatv\u00edzb\u0151l izol\u00e1lt DNS-mint\u00e1b\u00f3l real-time PCR-m\u00f3dszer seg\u00edts\u00e9g\u00e9vel, az 1 kromosz\u00f3m\u00e1n (1p36.11) tal\u00e1lhat\u00f3 RhD 7 exon kimutat\u00e1s\u00e1val meghat\u00e1rozt\u00e1k a magzat Rh-st\u00e1tus\u00e1t. Eredm\u00e9nyek: A real-time PCR-vizsg\u00e1lat 24 esetben azonos eredm\u00e9nyt adott a RhD 7-es exonj\u00e1ra vonatkoz\u00f3an a magzatv\u00edz-mint\u00e1kb\u00f3l, illetve az anyai v\u00e9rplazm\u00e1b\u00f3l izol\u00e1lt DNS eset\u00e9ben. Hat esetben a magzatv\u00edzb\u0151l izol\u00e1lt DNS pozit\u00edv, a plazm\u00e1b\u00f3l izol\u00e1lt szabad DNS vizsg\u00e1lata negat\u00edv eredm\u00e9nyt adott. A magzatv\u00edzb\u0151l t\u00f6rt\u00e9n\u0151 RhD-meghat\u00e1roz\u00e1s sor\u00e1n \u00f6t esetben nem, huszon\u00f6t esetben kimutathat\u00f3 volt az RhD-g\u00e9n 7 exonja. \n <jats:italic>K\u00f6vetkeztet\u00e9sek:<\/jats:italic>\n A real-time PCR egy viszonylag egyszer\u0171 \u00e9s megb\u00edzhat\u00f3 m\u00f3dszernek bizonyult az anyai v\u00e9rplazm\u00e1b\u00f3l t\u00f6rt\u00e9n\u0151 magzati v\u00e9rcsoport Rh-meghat\u00e1roz\u00e1s\u00e1ra. A m\u00f3dszer \u00e9rz\u00e9kenys\u00e9g\u00e9re vonatkoz\u00f3 eredm\u00e9nyek a szakirodalommal \u00f6sszhangban biztat\u00f3ak, a m\u00f3dszer \u00e9rz\u00e9kenys\u00e9ge t\u00f6bb marker felhaszn\u00e1l\u00e1s\u00e1val n\u00f6velhet\u0151. Tov\u00e1bbi, nagyobb esetsz\u00e1m\u00fa vizsg\u00e1lat elv\u00e9gz\u00e9se a m\u00f3dszer rutin klinikai gyakorlatban t\u00f6rt\u00e9n\u0151 alkalmaz\u00e1s\u00e1t is lehet\u0151v\u00e9 teszi.<\/jats:p>","DOI":"10.1556\/oh.2007.27904","type":"journal-article","created":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T19:17:41Z","timestamp":1174936661000},"page":"497-500","source":"Crossref","is-referenced-by-count":4,"title":["Non invasive detection of fetal Rh using real-time PCR method"],"prefix":"10.1556","volume":"148","author":[{"given":"Levente","family":"L\u00e1z\u00e1r","sequence":"first","affiliation":[{"name":"1 Semmelweis Egyetem, \u00c1ltal\u00e1nos Orvostudom\u00e1nyi Kar I. 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