{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T16:26:04Z","timestamp":1754151964242,"version":"3.41.2"},"reference-count":0,"publisher":"Codon Publications","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergol Immunopathol"],"abstract":"<jats:p>Introduction and Objectives: The degranulation and release of inflammatory mediators mediated by the high-affinity immunoglobulin E (IgE) receptor (Fc\u03b5RI) on mast cells in response to allergen contact is the driving force of anaphylaxis. This study shows that P815 cells, which were previously thought not to express Fc\u03b5RI, cause a reaction similar to Fc\u03b5RI-mediated degranulation in the presence of antigen and IgE.\nMaterials and Methods: The kinetics of degranulation were evaluated by comparing P815 cells with Fc\u03b5RI-expressing a rat basophilic leukemia (RBL-2H3) (that typically indicates the specific clone or subline within that cell line) cell lines. Degranulation activity was measured using the release rate of \u03b2-hexosaminidase as an indicator. P815 cells showed significant degranulation when compound 48\/80 or anti-dinitrophenyl (DNP)-IgE antibody and DNP-human serum albumin (HSA) antigen were added simultaneously. Gene expression analysis confirmed the expression of each Fc\u03b5RI subunit\u2014specifically, the \u03b3 subunit expressed markedly. Moreover, the expression of the phosphorylation enzymes Lyn, spleen tyrosine kinase (Syk), Fyn, and Bruton tyrosine kinase (Btk), which are involved in degranulation, was upregulated.\nResults: Fc\u03b5RI has three subunits: \u03b1, \u03b2, and \u03b3. P815 cells do not express Fc\u03b5RI because they have messenger ribonucleic acid (mRNA) for the \u03b3 subunit but not for the \u03b1 and \u03b2 subunits. However, P815 expressed each subunit protein (\u03b1, \u03b2, and \u03b3), as detected in the western blotting analysis of cell extracts in the presence of DNP-HSA antigen and anti-DNP-IgE.\nConclusion: These results suggest that P815 may cause degranulation via Fc\u03b5RI. Therefore, P815 is considered to be a cell model that can evaluate both Fc\u03b5RI-mediated and Fc\u03b5RI-independent degranulation reactions in response to allergens.<\/jats:p>","DOI":"10.15586\/aei.v53i4.1264","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:19:38Z","timestamp":1751361578000},"page":"119-127","source":"Crossref","is-referenced-by-count":0,"title":["Utility of mast cell P815 for evaluating Fc\u03b5RI-dependent and Fc\u03b5RI-independent allergic effects"],"prefix":"10.15586","volume":"52","author":[{"given":"Ryosuke","family":"Misawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhito","family":"Takemoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Iji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luo","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akari","family":"Koresawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6228","published-online":{"date-parts":[[2025,7,1]]},"container-title":["Allergologia et Immunopathologia"],"original-title":[],"link":[{"URL":"https:\/\/all-imm.com\/index.php\/aei\/article\/download\/1264\/2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/all-imm.com\/index.php\/aei\/article\/download\/1264\/2005","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/all-imm.com\/index.php\/aei\/article\/download\/1264\/2006","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/all-imm.com\/index.php\/aei\/article\/download\/1264\/2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T09:42:08Z","timestamp":1753090928000},"score":1,"resource":{"primary":{"URL":"https:\/\/all-imm.com\/index.php\/aei\/article\/view\/1264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.15586\/aei.v53i4.1264","relation":{},"ISSN":["0301-0546"],"issn-type":[{"type":"electronic","value":"0301-0546"}],"subject":[],"published":{"date-parts":[[2025,7,1]]}}}