{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T07:20:36Z","timestamp":1782890436198,"version":"3.54.5"},"reference-count":80,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tierarztl Prax Ausg K"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>Bei Hunden mit Verdacht auf Leptospirose ist es wichtig, eine eindeutige Diagnose zu stellen, um gezielt therapieren zu k\u00f6nnen. Zudem stellen infizierte Hunde ein Zoonoserisiko dar. Der kulturelle Erregernachweis aus Blut, Urin oder Gewebe gilt als diagnostischer Referenzstandard. Allerdings besitzt er eine geringe Sensitivit\u00e4t und Leptospiren ben\u00f6tigen Wochen bis Monate, um zu wachsen. Die Untersuchung auf Antik\u00f6rper und der Nachweis von Leptospiren-DNA mittels PCR geh\u00f6ren daher zu den wichtigsten Nachweismethoden in der Praxis. Beim Hund wird der Mikroagglutinationstest (MAT) trotz einiger Limitationen (z. B. negative Ergebnisse in fr\u00fchen Infektionsstadien, positive Resultate infolge impfinduzierter Antik\u00f6rper) nach wie vor am h\u00e4ufigsten durchgef\u00fchrt. ELISAs k\u00f6nnen zwischen IgG- und IgM-Antik\u00f6rpern differenzieren und so eine akute Infektion von fr\u00fcheren Impfungen oder persistierenden Infektionen abgrenzen. Inzwischen wurden ELISA-Schnelltests sowie In-House-PCR-Tests f\u00fcr die Praxis entwickelt, die einfache und schnelle Untersuchungen vor Ort erm\u00f6g lichen. Allerdings gibt es bisher erst wenige Daten zur Sensitivit\u00e4t und Spezifit\u00e4t dieser Tests. Eine sichere Diagnose kann nur bei einem positiven PCR-Ergebnis oder einem vierfachen Titeranstieg im MAT gestellt werden.<\/jats:p>","DOI":"10.15654\/tpk-170039","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T11:20:39Z","timestamp":1495020039000},"page":"170-177","source":"Crossref","is-referenced-by-count":4,"title":["Diagnose der Leptospirose beim Hund"],"prefix":"10.15654","volume":"45","author":[{"given":"Julia-Rebecca","family":"Llewellyn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Hartmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Bergmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2018,1,11]]},"reference":[{"key":"10.15654\/TPK-170039-1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.01.015"},{"key":"10.15654\/TPK-170039-2","doi-asserted-by":"crossref","unstructured":"Adler B. 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