{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:12:14Z","timestamp":1780413134664,"version":"3.54.1"},"reference-count":0,"publisher":"Shared Science Publishers OG","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CST"],"abstract":"<jats:p>Cells maintain their cytosolic calcium (Ca2+) in nanomolar range and use controlled increase in Ca2+ for intracellular signaling. With the extracellular Ca2+ in the millimolar range, there is a steep Ca2+ gradient across the plasma membrane (PM). Thus, injury that damages PM, leads to a cytosolic Ca2+ overload, which helps activate PM repair (PMR) response. However, in order to survive, the cells must cope with the Ca2+ overload. In a recent study (Chandra et al. J Cell Biol,doi: 10.1083\/jcb.202006035) we have examined how cells cope with injury-induced cytosolic Ca2+ overload. By monitoring Ca2+ dynamics in the cytosol and endoplasmic reticulum (ER), we found that PM injury-triggered increase in cytosolic Ca2+ is taken up by the ER. Pharmacological inhibition of ER Ca2+ uptake interferes with this process and compromises the repair ability of the injured cells. Muscle cells from patients and mouse model for the muscular dystrophy showed that lack of Anoctamin 5 (ANO5)\/Transmembrane protein 16E (TMEM16E), an ER-resident putative Ca2+-activated chloride channel (CaCC), are poor at coping with cytosolic Ca2+ overload. Pharmacological inhibition of CaCC and lack of ANO5, both prevent Ca2+ uptake into ER. These studies identify a requirement of Cl\u2013 uptake by the ER in sequestering injury-triggered cytosolic Ca2+ increase in the ER. Further, these studies show that ER helps injured cells cope with Ca2+ overload during PMR, lack of which contributes to muscular dystrophy due to mutations in the ANO5 protein.<\/jats:p>","DOI":"10.15698\/cst2021.05.249","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T10:42:01Z","timestamp":1620038521000},"page":"73-75","source":"Crossref","is-referenced-by-count":8,"title":["Coping with the calcium overload caused by cell injury: ER to the rescue"],"prefix":"10.15698","volume":"5","author":[{"given":"Goutam","family":"Chandra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davi A. G.","family":"M\u00e1zala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti K.","family":"Jaiswal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6394","published-online":{"date-parts":[[2021,5,10]]},"container-title":["Cell Stress"],"original-title":[],"link":[{"URL":"http:\/\/www.cell-stress.com\/wp-content\/uploads\/2021A-Chandra-Cell-Stress.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T10:42:05Z","timestamp":1620038525000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cell-stress.com\/researcharticles\/2021a-chandra-cell-stress\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,10]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5,10]]}},"URL":"https:\/\/doi.org\/10.15698\/cst2021.05.249","relation":{},"ISSN":["2523-0204"],"issn-type":[{"value":"2523-0204","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,10]]}}}