{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:56:58Z","timestamp":1781611018554,"version":"3.54.5"},"reference-count":68,"publisher":"Shared Science Publishers OG","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"Abstract<\/jats:title>\n \n Klebsiella pneumoniae<\/jats:italic>\n (Kp) is a Gram-negative bacillus responsible for approximately 10% of nosocomial bacterial infections and one-third of Gram-negative bacterial infections in hospitalized patients. The rise of multidrug-resistant and hypervirulent strains makes it a significant public health issue. This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Cl\u00ednico Universidad de Chile (HCUCH) 2021\u20132022 and explored associations with clinical characteristics. 45 CR-Kp strains from 29 patients in critical care units were analyzed. Mass spectrometry was used for species identification, and antimicrobial susceptibility was assessed by Kirby-Bauer disk diffusion. Clonality was determined using pulsed-field gel electrophoresis (PFGE), and multiplex PCR detected resistance and virulence genes. Clonal strains underwent whole-genome sequencing. PCR revealed the high prevalence of carbapenemase genes and extended-spectrum\n \n \n \u03b2<\/mml:mi>\n <\/mml:math>\n <\/jats:inline-formula>\n -lactamases. PFGE identified nine clones, corresponding to sequence types ST25, ST45, ST307, and ST1161. Frequent virulence factors included siderophores and adhesins, while the capsular serotype K2 was present in 44% of isolates. No classical hypervirulence markers were detected. The presence of\n blaKPC<\/jats:italic>\n correlated with more extended hospitalization. These findings reveal convergence between multidrug resistance and adaptive virulence traits rather than classical hypervirulence, highlighting evolving pathogenic strategies in high-risk CR-Kp clones circulating in Chile and emphasizing the need for enhanced molecular surveillance and infection protocols in critical care settings.\n <\/jats:p>","DOI":"10.15698\/mic2026.05.876","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:46:15Z","timestamp":1781610375000},"page":"186-197","source":"Crossref","is-referenced-by-count":0,"title":["Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae circulating in a Chilean tertiary-care hospital (2021\u20132022): Molecular characterization, resistance-virulence convergence, and clinical associations"],"prefix":"10.15698","volume":"13","author":[{"given":"Gustavo","family":"Araya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alhejandra","family":"\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Arellano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Bravo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Gajardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Barrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto M.","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"6394","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"issue":"1","key":"b00c19","first-page":"1","article-title":"Carbapenem Resistance: A Review","volume-title":"Med Sci","volume":"6","author":"Codjoe","year":"2017"},{"key":"b00c7c","article-title":"Estimating the economic costs of antimicrobial resistance : model and results","author":"Taylor","year":"2014"},{"key":"b00d1c","article-title":"The global burden of antimicrobial resistance","author":"O\u2019Leary","year":"2022","journal-title":"Nat Med"},{"key":"b00d67","first-page":"313","article-title":"Global economic impact of antibiotic resistance: A review","volume-title":"J Glob Antimicrob Resist","volume":"19","author":"Ahmad","year":"2019"},{"issue":"7","key":"b00dca","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.medin.2022.02.004","article-title":"Resistance mechanisms in Gram-negative bacteria","volume":"46","author":"Lepe","year":"2022","journal-title":"Med Intensiva Engl Ed"},{"issue":"6","key":"b00e30","doi-asserted-by":"crossref","first-page":"2888","DOI":"10.1128\/AAC.06301-11","article-title":"Carbapenem Therapy for Bacteremia Due to Extended-Spectrum-\u03b2-Lactamase-Producing Escherichia coli or Klebsiella pneumoniae: Implications of Ertapenem Susceptibility","volume":"56","author":"Lee","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"b00ed1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1086\/420816","article-title":"Antibiotic therapy for Klebsiella pneumoniae bacteremia: implications of production of extended-spectrum beta-lactamases","volume":"39","author":"Paterson","year":"2004","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"issue":"3","key":"b00fd5","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect Dis"},{"issue":"2","key":"b0102f","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01882-17","article-title":"Epidemiology of Carbapenem-Resistant Enterobacteriaceae Infections: Report from the China CRE Network","volume":"62","author":"Zhang","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"b01081","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3390\/antibiotics12020234","article-title":"Carbapenem-Resistant Klebsiella pneumoniae: Virulence Factors, Molecular Epidemiology and Latest Updates in Treatment Options","volume":"12","author":"Karampatakis","year":"2023","journal-title":"Antibiotics"},{"key":"b010f3","first-page":"18","article-title":"Systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant Klebsiella pneumoniae","volume-title":"Ann Clin Microbiol Antimicrob","volume":"16","author":"Xu","year":"2017"},{"issue":"6","key":"b01161","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.ijantimicag.2018.03.020","article-title":"Role of association of OmpK35 and OmpK36 alteration and blaESBL and\/or blaAmpC genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae","volume":"52","author":"Hamzaoui","year":"2018","journal-title":"Int J Antimicrob Agents"},{"issue":"11","key":"b01234","doi-asserted-by":"crossref","first-page":"4943","DOI":"10.1128\/AAC.00296-11","article-title":"Carbapenems: Past, Present, and Future","volume":"55","author":"Papp-Wallace","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"b012b4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40560-020-0429-6","article-title":"Molecular diversity of extended-spectrum \u03b2-lactamases and carbapenemases, and antimicrobial resistance","volume":"8","author":"Sawa","year":"2020","journal-title":"J Intensive Care"},{"issue":"10","key":"b01327","doi-asserted-by":"crossref","first-page":"693","DOI":"10.3390\/antibiotics9100693","article-title":"Molecular Epidemiology of Carbapenemases in Enterobacteriales from Humans, Animals, Food and the Environment","volume":"9","author":"Taggar","year":"2020","journal-title":"Antibiotics"},{"issue":"4","key":"b013a4","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/AAC.01451-07","article-title":"Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene","volume":"52","author":"Naas","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"b0143e","first-page":"11","article-title":"Travel-Related Antimicrobial Resistance: A Systematic Review","volume-title":"Trop Med Infect Dis","volume":"6","author":"Bokhary","year":"2021"},{"issue":"1","key":"b014c8","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1128\/AAC.05289-11","article-title":"Genetic features of the widespread plasmid coding for the carbapenemase OXA-48","volume":"56","author":"Poirel","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"b0153b","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1223138","article-title":"Molecular epidemiology, risk factors, and outcomes of carbapenem-resistant Klebsiella pneumoniae infection in a tertiary hospital in eastern China: for a retrospective study conducted over 4 years","volume":"14","author":"Cheng","year":"2023","journal-title":"Front Microbiol"},{"issue":"4","key":"b015b0","first-page":"288","article-title":"Emergence and clonal expansion of Klebsiella pneumoniae ST307, simultaneously producing KPC-3 and NDM-1","volume":"54","author":"Cejas","year":"2022","journal-title":"Rev Argent Microbiol"},{"issue":"4","key":"b01664","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1128\/CMR.05035-11","article-title":"Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions","volume":"25","author":"Tzouvelekis","year":"2012","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"b016f1","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01511-18","article-title":"Retrospective Observational Study from a Chinese Network of the Impact of Combination Therapy versus Monotherapy on Mortality from Carbapenem-Resistant Enterobacteriaceae Bacteremia","volume":"63","author":"Wang","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"b017f8","first-page":"2172","article-title":"A review: Virulence factors of Klebsiella pneumonia as emerging infection on the food chain","volume-title":"Vet World","volume":"15","author":"Riwu","year":"2022"},{"key":"b01885","article-title":"Klebsiella pneumoniae: an update on antibiotic resistance mechanisms","volume":"18","author":"Karami","year":"2023","journal-title":"Future Microbiol"},{"issue":"1","key":"b018fa","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev-micro-011420-075607","article-title":"Assembly of Bacterial Capsular Polysaccharides and Exopolysaccharides","volume":"74","author":"Whitfield","year":"2020","journal-title":"Annu Rev Microbiol"},{"issue":"2","key":"b0196d","first-page":"63","article-title":"Potential therapeutic targets of Klebsiella pneumoniae: a multi-omics review perspective","volume-title":"Brief Funct Genomics","volume":"21","author":"Ali","year":"2022"},{"issue":"3","key":"b019ed","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1080\/19490976.2018.1546519","article-title":"The bacterial siderophore enterobactin confers survival advantage to Salmonella in macrophages","volume":"10","author":"Saha","year":"2019","journal-title":"Gut Microbes"},{"issue":"9","key":"b01a94","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00776-18","article-title":"Identification of Biomarkers for Differentiation of Hypervirulent Klebsiella pneumoniae from Classical K. pneumoniae","volume":"56","author":"Russo","year":"2018","journal-title":"J Clin Microbiol"},{"issue":"2","key":"b01b5e","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02867-23","article-title":"Differentiation of hypervirulent and classical Klebsiella pneumoniae with acquired drug resistance","volume":"15","author":"Russo","year":"2024","journal-title":"mBio"},{"issue":"2","key":"b01c5b","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2023.2271096","article-title":"Convergent Klebsiella pneumoniae strains belonging to a sequence type 307 outbreak clone combine cefiderocol and carbapenem resistance with hypervirulence","volume":"12","author":"Schaufler","year":"2023","journal-title":"Emerg Microbes Infect"},{"issue":"2","key":"b01d4b","doi-asserted-by":"crossref","first-page":"224","DOI":"10.4067\/S0716-10182012000200018","article-title":"Primer caso de detecci\u00f3n de blaKpc en Chile: desde Italia a un hospital p\u00fablico de Santiago","volume":"29","author":"Cifuentes","year":"2012","journal-title":"Rev Chil Infectol"},{"key":"b01e26","article-title":"Bolet\u00edn de Resistencia Antimicrobiana","author":"Instituto de Salud P\u00fablica\u00a0de Chile","year":"2022"},{"issue":"5","key":"b01e68","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00399-23","article-title":"Antimicrobial resistance, pathogenic potential, and genomic features of carbapenem-resistant Klebsiella pneumoniae isolated in Chile: high-risk ST25 clones and novel mobile elements","volume":"11","author":"Veloso","year":"2023","journal-title":"Microbiol Spectr"},{"issue":"10","key":"b01f4b","doi-asserted-by":"crossref","DOI":"10.3201\/eid2210.160695","article-title":"Polymyxin B Resistance in Carbapenem-Resistant Klebsiella pneumoniae, S\u00e3o Paulo, Brazil","volume":"22","author":"Bartolleti","year":"2016","journal-title":"Emerg Infect Dis J"},{"issue":"3","key":"b0201e","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.ijantimicag.2012.05.006","article-title":"Molecular correlates of the spread of KPC-producing Enterobacteriaceae in Colombia","volume":"40","author":"Mojica","year":"2012","journal-title":"Int J Antimicrob Agents"},{"key":"b020df","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2020.616490","article-title":"Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales","volume":"7","author":"Bonnin","year":"2021","journal-title":"Front Med"},{"key":"b0216b","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3389\/fmicb.2016.00895","article-title":"Global Dissemination of Carbapenemase-Producing Klebsiella pneumoniae: Epidemiology, Genetic Context, Treatment Options, and Detection Methods","volume":"7","author":"Lee","year":"2016","journal-title":"Front Microbiol"},{"issue":"10","key":"b021ff","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01148-20","article-title":"Emerging Antimicrobial-Resistant High-Risk Klebsiella pneumoniae Clones ST307 and ST147","volume":"64","author":"Peirano","year":"2020","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"b02277","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1089\/mdr.2019.0433","article-title":"Characterization of blaKPC-2-Harboring Klebsiella pneumoniae Isolates and Mobile Genetic Elements from Outbreaks in a Hospital in Ecuador","volume":"27","author":"Reyes","year":"2021","journal-title":"Microb Drug Resist Larchmt N"},{"issue":"1","key":"b0231e","first-page":"3","article-title":"Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolated from patients in two public hospitals in Carabobo and Zulia states, Venezuela","volume":"58","author":"Falco","year":"2017","journal-title":"Invest Clin"},{"key":"b023ab","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.nmni.2018.02.002","article-title":"Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections","volume":"23","author":"Garza-Ramos","year":"2018","journal-title":"New Microbes New Infect"},{"issue":"1","key":"b02476","doi-asserted-by":"crossref","first-page":"4188","DOI":"10.1038\/s41467-021-24448-3","article-title":"A genomic surveillance framework and genotyping tool for Klebsiella pneumoniae and its related species complex","volume":"12","author":"Lam","year":"2021","journal-title":"Nat Commun"},{"issue":"7","key":"b0250d","doi-asserted-by":"crossref","first-page":"4351","DOI":"10.1007\/s00203-021-02425-0","article-title":"Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil","volume":"203","author":"Nakamura-Silva","year":"2021","journal-title":"Arch Microbiol"},{"key":"b025a7","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jgar.2016.01.001","article-title":"Molecular typing and genetic environment of the blaKPC gene in Chilean isolates of Klebsiella pneumoniae","volume":"4","author":"Barr\u00eda-Loaiza","year":"2016","journal-title":"J Glob Antimicrob Resist"},{"key":"b026b3","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2018.00324","article-title":"Genetic Platforms of blaCTX-M in Carbapenemase-Producing Strains of K. pneumoniae Isolated in Chile","volume":"9","author":"Carrasco-Anabal\u00f3n","year":"2018","journal-title":"Front Microbiol"},{"issue":"10","key":"b02778","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1086\/605922","article-title":"Attributable mortality rate for carbapenem-resistant Klebsiella pneumoniae bacteremia","volume":"30","author":"Borer","year":"2009","journal-title":"Infect Control Hosp Epidemiol"},{"issue":"2","key":"b0282c","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.medmal.2013.11.007","article-title":"Carbapenemase-producing Enterobacteriaceae: overview of a major public health challenge","volume":"44","author":"Nordmann","year":"2014","journal-title":"Med Mal Infect"},{"issue":"3","key":"b02885","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/cid\/ciw741","article-title":"Comparing the Outcomes of Patients With Carbapenemase-Producing and Non-Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae Bacteremia","volume":"64","author":"Tamma","year":"2017","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"b0292c","article-title":"M100 performance standards for antimicrobial susceptibility testing","author":"CLSI","year":"2023"},{"key":"b02971","first-page":"e65","article-title":"Consenso latinoamericano para definir, categorizar y notificar pat\u00f3genos multirresistentes, con resistencia extendida o panresistentes","volume":"43","author":"Jim\u00e9nez\u00a0Pearson","year":"2019","journal-title":"Rev Panam Salud P\u00fablica"},{"key":"b02a1f","article-title":"FastQC: A Quality Control Tool for High Throughput Sequence Data","author":"Andrews","year":"2010"},{"issue":"1","key":"b02a5f","doi-asserted-by":"crossref","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes De Novo Assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinforma"},{"issue":"8","key":"b02ae7","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinforma Oxf Engl"},{"issue":"14","key":"b02b67","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinforma Oxf Engl"},{"key":"b02bc0","article-title":"ABRicate: Mass screening of contigs for antimicrobial and virulence genes","author":"Seemann","year":"2018"},{"issue":"11","key":"b02c00","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00483-19","article-title":"Validating the AMRFinder Tool and Resistance Gene Database by Using Antimicrobial Resistance Genotype-Phenotype Correlations in a Collection of Isolates","volume":"63","author":"Feldgarden","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"b02d24","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J Antimicrob Chemother"},{"issue":"D1","key":"b02dd8","doi-asserted-by":"crossref","first-page":"D737","DOI":"10.1093\/nar\/gkt1252","article-title":"BacMet: antibacterial biocide and metal resistance genes database","volume":"42","author":"Pal","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"b02e65","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: hierarchical and refined dataset for big data analysis\u201310 years on","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"b02ef2","article-title":"MLST: Scan contig files against PubMLST typing schemes","author":"Seemann","year":"2018"},{"issue":"1","key":"b02f32","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/1471-2105-11-595","article-title":"BIGSdb: Scalable analysis of bacterial genome variation at the population level","volume":"11","author":"Jolley","year":"2010","journal-title":"BMC Bioinformatics"},{"issue":"7","key":"b02f95","doi-asserted-by":"crossref","DOI":"10.1093\/molbev\/msac135","article-title":"A Dual Barcoding Approach to Bacterial Strain Nomenclature: Genomic Taxonomy of Klebsiella pneumoniae Strains","volume":"39","author":"Hennart","year":"2022","journal-title":"Mol Biol Evol"},{"issue":"3","key":"b03037","doi-asserted-by":"crossref","DOI":"10.1099\/mgen.0.000166","article-title":"chewBBACA: A complete suite for gene-by-gene schema creation and strain identification","volume":"4","author":"Silva","year":"2018","journal-title":"Microb Genomics"},{"issue":"22","key":"b030e6","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"issue":"7","key":"b031b4","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1093\/molbev\/msp077","article-title":"FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix","volume":"26","author":"Price","year":"2009","journal-title":"Mol Biol Evol"},{"issue":"W1","key":"b03227","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"b0328d","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/bioinformatics\/btw582","article-title":"PHYLOViZ 2.0: providing scalable data integration and visualization for multiple phylogenetic inference methods","volume":"33","author":"Nascimento","year":"2017","journal-title":"Bioinformatics"},{"issue":"1","key":"b03327","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2105-10-152","article-title":"Global optimal eBURST analysis of multilocus typing data using a graphic matroid approach","volume":"10","author":"Francisco","year":"2009","journal-title":"BMC Bioinformatics"}],"container-title":["Microbial Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/microbialcell.com\/wordpress\/wp-content\/uploads\/2026a-araya-microbial-cell.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:46:26Z","timestamp":1781610386000},"score":1,"resource":{"primary":{"URL":"http:\/\/microbialcell.com\/researcharticles\/2026a-araya-microbial-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,21]]},"references-count":68,"URL":"https:\/\/doi.org\/10.15698\/mic2026.05.876","relation":{},"ISSN":["2311-2638"],"issn-type":[{"value":"2311-2638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,21]]}}}