{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:38:43Z","timestamp":1790123923153,"version":"4.0.1"},"reference-count":21,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Background and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg\/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5\u2032-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 \u00b5g\/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.<\/jats:p>","DOI":"10.1590\/0001-3765201420130005","type":"journal-article","created":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T13:34:35Z","timestamp":1400160875000},"page":"801-806","source":"Crossref","is-referenced-by-count":21,"title":["Natural sesquiterpen lactones as acetylcholinesterase inhibitors"],"prefix":"10.1590","volume":"86","author":[{"given":"HOMA","family":"HAJIMEHDIPOOR","sequence":"first","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MAHMOUD","family":"MOSADDEGH","sequence":"additional","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences, Iran; Shahid Beheshti University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FARZANEH","family":"NAGHIBI","sequence":"additional","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences, Iran; Shahid Beheshti University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALI","family":"HAERI","sequence":"additional","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARYAM","family":"HAMZELOO-MOGHADAM","sequence":"additional","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"a006395","DOI":"10.1101\/cshperspect.a006395","article-title":"Pharmacologic Treatment for Alzheimer Disease","volume":"2","author":"Aisen PS","year":"2012","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"(2)","key":"ref2","first-page":"7","article-title":"Alzheimer's disease facts and figures","volume":"8","year":"2012","journal-title":"Alzheimer's & Dementia"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.3390\/molecules17033082","article-title":"Anti-acetylcholinesterase and antioxidant activity of essential oils from Hedychium gardnerianum Sheppard ex Ker-Gawl","volume":"17","author":"Arruda M","year":"2012","journal-title":"Molecules"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1080\/14786410500185303","article-title":"Bisbenzylisoquinoline alkaloids from Cocculuspendulus","volume":"23","author":"Atta-Ur-Rahman","year":"2009","journal-title":"Nat Prod Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.steroids.2004.03.016","article-title":"New pregnane-type steroidal alkaloids from Sarcoccasaligna and their cholinesterase inhibitory activity","volume":"69","author":"Atta-Ur-Rahman","year":"2004","journal-title":"Steroids"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"9205","DOI":"10.3390\/molecules15129205","article-title":"Alkaloids from stems of Esenbeckialeiocarpa Engl. (Rutaceae) as potential treatment for Alzheimer disease","volume":"15","author":"Cardoso-Lopes EM","year":"2010","journal-title":"Molecules"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1002\/cbdv.200690102","article-title":"New cholinesterase-inhibiting triterpenoid alkaloids from Buxushyrcana","volume":"3","author":"Choudhary MI","year":"2006","journal-title":"Chem Biodivers"},{"issue":"(6)","key":"ref8","first-page":"1","article-title":"Epigenetic Regulation of Fatty Acid Amide Hydrolase in Alzheimer Disease","volume":"7","author":"D'Addario","year":"2012","journal-title":"PLoS ONE"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman GL","year":"1961","journal-title":"Biochem Pharmacol"},{"key":"ref10","first-page":"5","article-title":"Rivastigmine: an open-label, observational study of safety and effectiveness in treating patients with Alzheimer's disease for up to 5 years","author":"Farlow MR","year":"2005","journal-title":"BMC Geriatrics"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1590\/S1519-69842011000400025","article-title":"Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants","volume":"71","author":"Feitosa CM","year":"2011","journal-title":"Braz J Biol"},{"key":"ref12","author":"Frydman-Marom A","year":"2011"},{"key":"ref13","first-page":"979","article-title":"Anti-proliferative activity and apoptotic potential of britannin, a sesquiterpene lactone from Inulaaucheriana","volume":"7","author":"Hamzeloo-Moghadam M","year":"2012","journal-title":"Nat Prod Commun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/1752-153X-7-116","article-title":"Acetyl and butyryl cholinesterase inhibitory sesquiterpene lactones from Amberboaramosa","volume":"7","author":"Ibrahim M","year":"2013","journal-title":"Chem Cent J"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1136\/jnnp.74.11.1567","article-title":"A randomised placebo controlled study to assess the effects of cholinergic treatment on muscarinic receptors in Alzheimer's disease","volume":"74","author":"Kemp PM","year":"2003","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1515\/znc-2007-1-225","article-title":"New Findings about Ellman's Method to Determine Cholinesterase Activity Z","volume":"62c","author":"Komersova A","year":"2007","journal-title":"Naturforsch"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1002\/jsfa.5708","article-title":"Evaluation of Citrus aurantifoliapeel and leaves extracts for their chemical composition, antioxidant and anti-cholinesterase activities","volume":"92","author":"Loizzo MR","year":"2012","journal-title":"J Sci Food Agric"},{"key":"ref18","first-page":"511","article-title":"Sesquiterpene lactones from Inula oculus-christi","volume":"5","author":"Mosaddegh M","year":"2010","journal-title":"Nat Prod Commun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1002\/ptr.2224","article-title":"Screening of Indian Medicinal Plants for Acetylcholinesterase Inhibitory Activity","volume":"21","author":"Mukherjee PK","year":"2007","journal-title":"Phytother Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2174\/157488909787002582","article-title":"Contemporary anticholinesterase pharmaceuticals of natural origin and their synthetic analogues for the treatment of alzheimer's disease","volume":"4","author":"Orhan G","year":"2009","journal-title":"Recent Pat CNS Drug Discov"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1016\/j.bmc.2005.04.030","article-title":"New selective acetylcholinesterase inhibitors designed from natural piperidine alkaloids","volume":"13","author":"Viegas Jr C","year":"2005","journal-title":"Bioorg Med Chem"}],"container-title":["Anais da Academia Brasileira de Ci\u00eancias"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/aabc\/v86n2\/0001-3765-aabc-0001-3765201420130005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T13:49:23Z","timestamp":1565444963000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0001-37652014000200801&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["S0001-37652014000200801"],"URL":"https:\/\/doi.org\/10.1590\/0001-3765201420130005","relation":{},"ISSN":["0001-3765"],"issn-type":[{"value":"0001-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]}}}