{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T06:45:13Z","timestamp":1794465913719,"version":"4.9.1"},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>The production of biomass by microalgae is considered a clean alternative compared to other plant crops that require large areas for cultivation and that generate environmental impacts. This study evaluated the influence of temperature and nutrients on lipid contents of cultured species of freshwater microalgae, with a view toward using these lipids for biodiesel production. Two strains of Monoraphidium contortum, a culture containing Chlorella vulgaris and Desmodesmus quadricauda and another strain of Microcystis aeruginosa were maintained in the laboratory for six days, in five culture media: modified ASM-1 (control, with high concentrations of phosphate and nitrate; phosphorus-deficient; non-limiting phosphate; nitrogen-deficient; and non-limiting nitrate). The cultures were then exposed to temperatures of 13\u00b0C, 25\u00b0C (control) and 37\u00b0C for eight days (n=3). Lipids were extracted by the cold-solvent (methanol and chloroform) method. On average, the highest total lipid yields were observed when the strains were maintained at 13\u00b0C and in the non-limiting nitrate medium. The lipid percentage varied depending on the concentration of algal biomass. This study showed that manipulation of controlling factors can increase the lipid concentration, optimizing the total production in order to use this raw material for biodiesel.<\/jats:p>","DOI":"10.1590\/0001-3765201420130136","type":"journal-article","created":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T16:31:18Z","timestamp":1410193878000},"page":"1239-1248","source":"Crossref","is-referenced-by-count":29,"title":["Influence of temperature and nutrient content on lipid production in freshwater microalgae cultures"],"prefix":"10.1590","volume":"86","author":[{"given":"JULIANA E.","family":"BOHNENBERGER","sequence":"first","affiliation":[{"name":"Universidade do Vale do Rio dos Sinos,  Brazil; Universidade Federal do Rio Grande do Sul,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LUCIANE O.","family":"CROSSETTI","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Rio dos Sinos,  Brazil; Universidade Federal do Rio Grande do Sul,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Produ\u00e7\u00e3o de toxina por Microcystis aeruginosa em diferentes fases de cultivo e concentra\u00e7\u00f5es de nitrog\u00eanio","first-page":"6","author":"Aguiar DG","year":"1992"},{"key":"ref2","series-title":"Alimentos\u2019e\u2019Bebidas Produzidos por Fermenta\u00e7\u00e3o","first-page":"210","volume-title":"Vinagres","author":"Aquarone E","year":"1983"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1745-4522.1996.tb00073.x","article-title":"Microalgae as a source of fatty acids","volume":"3","author":"Behrens PW","year":"1996","journal-title":"J Food Lipids"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method for total lipid extraction and purification","volume":"37","author":"Bligh EG","year":"1959","journal-title":"Can J Biochem Physiol"},{"key":"ref5","first-page":"35","article-title":"Potencial de absor\u00e7\u00e3o de carbono por esp\u00e9cies de microalgas usadas na aq\u00fcicultura: primeiros passos para o desenvolvimento de um \u201cMecanismo de Desenvolvimento Limpo\u201d","volume":"29","author":"Borges L","year":"2007","journal-title":"Atl\u00e2ntica"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.rser.2009.10.009","article-title":"Biofuels from microalgae. A review of technologies for production, processing and extractions of biofuels and co-products","volume":"14","author":"Brennan L","year":"2010","journal-title":"Renew Sustain Energy Rev"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1021\/ef9003818","article-title":"Alga-Based Biodiesel Production and Optimization Using Sugar Cane as the Feedstock","volume":"23","author":"Cheng Y","year":"2009","journal-title":"Energ Fuel"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.biotechadv.2007.02.001","article-title":"Biodiesel from microalgae","volume":"25","author":"Chisti Y","year":"2007","journal-title":"Biotechnol Adv"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1590\/S0001-37652011000400019","article-title":"Cyanobacterial blooms in stratified and destratified eutrophic reservoirs in semi-arid region of Brazil","volume":"83","author":"Dantas EW","year":"2011","journal-title":"An Acad Bras Cienc"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jbiosc.2012.05.007","article-title":"Lipid accumulation and growth characteristics of <em>Chlorella zofingiensis<\/em> under different nitrate and phosphate concentrations","volume":"114","author":"Feng P","year":"2012","journal-title":"J Biosci Bioeng"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1590\/S0100-40422009000300004","article-title":"Biomassa e energia","volume":"32","author":"Goldemberg J","year":"2009","journal-title":"Qu\u00edm Nova"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s10811-008-9392-7","article-title":"Lipid productivity as a key characteristic for choosing algal species for biodiesel production","volume":"21","author":"Griffiths MJ","year":"2009","journal-title":"J Appl Phycol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/447520a","article-title":"Algae bloom again","volume":"447","author":"Haag AL","year":"2007","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.biochi.2008.11.004","article-title":"The versatility of algae and their lipid metabolism","volume":"91","author":"Harwood JL","year":"2009","journal-title":"Biochimie"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1046\/j.1529-8817.1999.3520403.x","article-title":"Biovolume calculation for pelagic and benthic microalgae","volume":"35","author":"Hillebrand H","year":"1999","journal-title":"J Phycol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.apenergy.2009.06.016","article-title":"Biodiesel production by microalgal biotechnology","volume":"87","author":"Huang G","year":"2010","journal-title":"Appl Energy"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0141-0229(00)00266-0","article-title":"Increase in <em>Chlorella<\/em> strains calorific values when grown in low nitrogen medium","volume":"27","author":"Illman AM","year":"2000","journal-title":"Enzyme Microb Technol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/BF00007865","article-title":"The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting","volume":"11","author":"Lund JWG","year":"1958","journal-title":"Hydrobiologia"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00253-009-1935-6","article-title":"Microalga <em>Scenedesmus obliquus<\/em> as a potential source for biodiesel production","volume":"84","author":"Mandal S","year":"2009","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.rser.2009.07.020","article-title":"Microalgae for biodiesel production and other applications: A review","volume":"14","author":"Mata TM","year":"2010","journal-title":"Renew Sustain Energy Rev"},{"key":"ref21","series-title":"Prospecttive della Ricerche","first-page":"111","volume-title":"Composizione biochimica generale delle biomasse di <em>Spirulina platensis<\/em> e <em>Spirulina maxima<\/em>","author":"Paoletti C","year":"1980"},{"key":"ref22","first-page":"179","article-title":"Perspectivas do biodiesel no Brasil","volume":"30","author":"Pl\u00e1 J\u00c1","year":"2002","journal-title":"Indic Econ"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0176-1617(11)80732-7","article-title":"Physiological and biochemical characteristics of a copper tolerant and a wild type strain of <em>Anabaena doliolum<\/em> under copper stress","volume":"138","author":"Rai LC","year":"1991","journal-title":"J Plant Physiol"},{"key":"ref24","author":"Reynolds CS","year":"1984"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4319\/lo.1978.23.1.0010","article-title":"Effect of N:P atomic ratio and nitrate limitation on algal growth, cell composition and nitrate uptake","volume":"23","author":"Rhee GY","year":"1978","journal-title":"Limnol Oceanogr"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.0022-3646.1981.00374.x","article-title":"Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light dark cycles","volume":"17","author":"Shiflin NS","year":"1981","journal-title":"J Phycol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.tree.2009.11.007","article-title":"The ecology of algal biodiesel production","volume":"25","author":"Smith VH","year":"2010","journal-title":"Trends in Ecol Evol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1263\/jbb.101.87","article-title":"Commercial applications of microalgae","volume":"101","author":"Sporalore P","year":"2006","journal-title":"J Biosci Bioeng"},{"key":"ref29","series-title":"Anais do XVI Encontro de Qu\u00edmica da Regi\u00e3o Sul","first-page":"676","volume-title":"Determina\u00e7\u00e3o do teor de lip\u00eddios de microalgas visando \u00e0 obten\u00e7\u00e3o de biodiesel","author":"Tomasini D","year":"2008"},{"key":"ref30","first-page":"1","article-title":"Zur Vervolkommnung der quantitativen Phytoplankton-Methodik","volume":"9","author":"Uterm\u00f6hl H","year":"1958","journal-title":"Mitt Int Ver Theor Angew Limnol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10811-009-9446-5","article-title":"Biofuels, facts, fantasy, and feasibility","volume":"21","author":"Walker DA","year":"2009","journal-title":"J Appl Phycol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0044-8486(83)90296-X","article-title":"Nutritional values of live organisms used in Japan for mass propagation of fish: A review","volume":"34","author":"Watanabe T","year":"1983","journal-title":"Aquaculture"}],"container-title":["Anais da Academia Brasileira de Ci\u00eancias"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/aabc\/v86n3\/0001-3765-aabc-86-03-1239.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,22]],"date-time":"2017-05-22T23:55:41Z","timestamp":1495497341000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0001-37652014000301239&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["S0001-37652014000301239"],"URL":"https:\/\/doi.org\/10.1590\/0001-3765201420130136","relation":{},"ISSN":["0001-3765"],"issn-type":[{"value":"0001-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]}}}