{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:25:47Z","timestamp":1759638347950,"version":"3.41.0"},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"abstract":"<jats:p>ABSTRACT Ottonia martiana is a plant popularly known in Brazil by the use for toothache. Ethanolic extract (EE), hexane fraction (HF), dichloromethane fraction (DF) and piperovatine obtained from O. martiana were assayed in vitro and in vivo. The acute toxicity of EE was determined, and LD50 values of 164.5 and 65.0 mg\/kg by the oral and intraperitoneal routes, respectively, indicated a high toxicity for EE in vivo, explaining its popular use by topical administration only. A local anesthetic-like effect of EE and its fractions was observed in experimental models using pain induction, and such effect involved an analgesic action. The antimycobacterial activity of EE, HF, DF and piperovatine was evaluated against Mycobacterium tuberculosis H37Rv ATCC 27924. EE, HF, DF, and piperovatine showed a potential antimycobacterial effect with MICs of 16.0, 62.0, 62.0 and 8.0 \u03bcg\/mL, respectively. Piperovatine was more effective than the EE or the other fractions. The selectivity index (SI=IC50\/MIC) values calculated for EE, HF, DF and piperovatine based on the MICs and the cytotoxicity against J774 macrophages (IC50 by MTT assay) revealed values of 6.43, 2.34, 1.5 and 9.66, respectively.<\/jats:p>","DOI":"10.1590\/0001-3765201520140090","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T22:58:43Z","timestamp":1448492323000},"page":"1991-2000","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae)"],"prefix":"10.1590","volume":"87","author":[{"given":"MIRIAM M.","family":"CUNICO","sequence":"first","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HERBERT A.","family":"TREBIEN","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00c1BIO C.","family":"GALETTI","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OBDULIO G.","family":"MIGUEL","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARILIS D.","family":"MIGUEL","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CELSO G.","family":"AUER","sequence":"additional","affiliation":[{"name":"Embrapa Florestas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00c9LIO L.","family":"SILVA","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANA OL\u00cdVIA DE","family":"SOUZA","sequence":"additional","affiliation":[{"name":"Instituto Butantan, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2015,11,27]]},"reference":[{"key":"ref1","first-page":"57","article-title":"In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogens in Iraq","volume":"32","author":"Al-Bayati FA","year":"2008","journal-title":"Turk J Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/S0022-3565(25)07892-9","article-title":"Biological comparison of local anesthetics","volume":"85","author":"B\u00fclbring E","year":"1945","journal-title":"J Pharmacol Exp Ther"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1590\/S1806-83242007000100008","article-title":"Antimicrobial activity of Uncaria tomentosa against oral human pathogens","volume":"21","author":"Ccahuana-Vasquez RA","year":"2007","journal-title":"Braz Oral Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/0031-9422(81)80027-1","article-title":"Amides of Ottonia corcovadensis","volume":"20","author":"Costa SS","year":"1981","journal-title":"Phytochemistry"},{"key":"ref5","first-page":"746","article-title":"Antimicrobial and allelopathic potential of the amides isolated from the roots of Ottonia martiana Miq., Piperaceae","volume":"29","author":"Cunico MM","year":"2006","journal-title":"Quim Nova"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1590\/S0103-50532007000100021","article-title":"Phytochemical and antibacterial evaluation of essential oils from Ottonia martiana Miq. (Piperaceae)","volume":"18","author":"Cunico MM","year":"2007","journal-title":"J Braz Chem Soc"},{"key":"ref7","article-title":"Extraction of sterols in fruits of Ottonia martiana Miq., Piperaceae, with liquefied gas","author":"Cunico MM","year":"2003","journal-title":"Quim Nova"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-1759(86)90368-6","article-title":"Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability","volume":"89","author":"Denizot F","year":"1986","journal-title":"J Immun Methods"},{"key":"ref9","series-title":"Plant biodiversity-evolutionary and ecological perspectives","first-page":"1","author":"Elmqvist T","year":"1997"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/S0014-827X(01)01092-8","article-title":"Local anaesthetic activity of beta-caryophyllene","volume":"56","author":"Ghelardini C","year":"2001","journal-title":"Farmaco"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1055\/s-2007-971526","article-title":"(2E,4E)-N-isobutyl-9-piperonyl-nona-2,4-dienoic amide from Ottonia anisum","volume":"43","author":"Giesbrecht AM","year":"1981","journal-title":"Planta Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1875-595X.2002.tb00638.x","article-title":"Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms","volume":"52","author":"Groppo FC","year":"2002","journal-title":"Int Dent J"},{"key":"ref13","series-title":"Piper\u00e1ceas. 1. Ottonia. 2. Sarcorachis. 3. Potomorphe","first-page":"3","author":"Guimar\u00e3es EF","year":"1978"},{"key":"ref14","series-title":"Human medicinal agents from plants","first-page":"1","volume":"534","author":"Kinghorn AD","year":"1993"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0022-3565(25)03549-9","article-title":"A simplified method of evaluating dose-effect experiments","volume":"96","author":"Litchfield JT","year":"1949","journal-title":"J Pharmacol Exp Ther"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0378-8741(83)90022-3","article-title":"Piperovatine, the tongue-numbing principle of Ottonia frutescens","volume":"7","author":"Makapugay HC","year":"1983","journal-title":"J Ethnopharmacol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0378-8741(02)00224-6","article-title":"In vitro neuropharmacological evaluation of piperovatine, an isobutylamide from Piper piscatorum (Piperaceae)","volume":"83","author":"McFerren MA","year":"2002","journal-title":"J Ethnopharmacol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-8741(97)00154-2","article-title":"Piscicidal properties of piperovatine from Piper piscatorum (Piperaceae)","volume":"60","author":"McFerren MA","year":"1998","journal-title":"J Ethnopharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1128\/AAC.46.8.2720-2722.2002","article-title":"Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis","volume":"46","author":"Palomino JC","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1021\/jo00833a022","article-title":"Chemistry of Ottonia vahlii. II. Constitution of the nonvolatile component","volume":"35","author":"Price SJ","year":"1970","journal-title":"J Org Chem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1039\/p19820001493","article-title":"Isolation and identification of amides from Piper callosum. synthesis of pipercallosine and pipercallosidine","volume":"1","author":"Pring BG","year":"1982","journal-title":"J Chem Soc Perkin Trans"},{"year":"2001","series-title":"Pharmacology","author":"Rang H","key":"ref22"},{"key":"ref23","first-page":"113","article-title":"Investigation of local anesthetic effect and toxicity of Ottonia propinqua (Piperaceae)","volume":"16","author":"Rates SMK","year":"1997","journal-title":"Acta Farm Bonaerense"},{"key":"ref24","first-page":"77","article-title":"Piperlonguminina y estigmasterol, compuestos de ra\u00edces y tallos de Piper auritum, actividad insecticida de extractos","volume":"27","author":"S\u00e1ez J","year":"1998","journal-title":"Rev Colomb Quim"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/0028-3908(92)90057-V","article-title":"The capsaicin test in mice for evaluating tachykinin antagonists in the spinal cord","volume":"31","author":"Sakurada T","year":"1992","journal-title":"Neuropharmacology"},{"key":"ref26","first-page":"28","article-title":"Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey","volume":"1","author":"Sarac N","year":"2007","journal-title":"Eur Asia J Bio Sci"},{"key":"ref27","series-title":"M\u00e9todos de avalia\u00e7\u00e3o de atividade biol\u00f3gica de produtos naturais e sint\u00e9ticos","first-page":"108","author":"Souza MM","year":"2003"},{"key":"ref28","series-title":"Cough induced by mechanical stimulation","first-page":"374","author":"Vogel HG","year":"2002"},{"year":"2002","series-title":"General guidelines for methodologies on research and evaluation of traditional medicine","key":"ref29"},{"year":"2002","series-title":"New food and pharmaceutical products from agriculture","author":"Wilkinson JA","key":"ref30"}],"container-title":["Anais da Academia Brasileira de Ci\u00eancias"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/aabc\/v87n4\/0001-3765-aabc-201520140090.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T15:07:14Z","timestamp":1748704034000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0001-37652015000501991&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,27]]},"references-count":30,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,12]]}},"alternative-id":["S0001-37652015000501991"],"URL":"https:\/\/doi.org\/10.1590\/0001-3765201520140090","relation":{},"ISSN":["1678-2690","0001-3765"],"issn-type":[{"type":"electronic","value":"1678-2690"},{"type":"print","value":"0001-3765"}],"subject":[],"published":{"date-parts":[[2015,11,27]]}}}