{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T21:20:12Z","timestamp":1773436812196,"version":"3.50.1"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"abstract":"<jats:p>The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.<\/jats:p>","DOI":"10.1590\/0001-3765201520140122","type":"journal-article","created":{"date-parts":[[2015,2,21]],"date-time":"2015-02-21T14:54:45Z","timestamp":1424530485000},"page":"455-462","source":"Crossref","is-referenced-by-count":8,"title":["Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis"],"prefix":"10.1590","volume":"87","author":[{"given":"JULIANA L.","family":"DREYFUSS","sequence":"first","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil; Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CAIO V.","family":"REGATIERI","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil; Universidade Federal de S\u00e3o Paulo,  Brasil; New England Eye Center,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BRUNO","family":"COELHO","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOS\u00c9 B.","family":"BARBOSA","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DENISE DE","family":"FREITAS","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HELENA B.","family":"NADER","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JO\u00c3O R.","family":"MARTINS","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brasil; Universidade Federal de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2015,2,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1097\/00003226-200108000-00013","article-title":"Tear IgA and serum IgG antibodies against Acanthamoeba in patients with Acanthamoeba keratitis","volume":"20","author":"ALIZADEH H","year":"2001","journal-title":"Cornea"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/0002-9394(81)90650-4","article-title":"Lysozyme content of tears in some external eye infections","volume":"92","author":"AVISAR R","year":"1981","journal-title":"Am J Ophthalmol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1001\/archopht.124.10.1451","article-title":"Altered tear composition in smokers and patients with graves ophthalmopathy","volume":"124","author":"BAKER GR","year":"2006","journal-title":"Arch Ophthalmol"},{"key":"ref4","first-page":"543","article-title":"Tear lactoferrin levels in patients with external inflammatory ocular disease","volume":"28","author":"BALLOW M","year":"1987","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1097\/01.ico.0000170693.13326.fb","article-title":"Adenoviral keratoconjunctivitis in a tertiary care eye clinic","volume":"25","author":"BUTT AL","year":"2006","journal-title":"Cornea"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1097\/OPX.0b013e318181ae4f","article-title":"Tear lipocalin and lysozyme in Sjogren and non-Sjogren dry eye","volume":"85","author":"CAFFERY B","year":"2008","journal-title":"Optom Vis Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1590\/S0036-46651992000100004","article-title":"Comparative study of adenoviruses with monoclonal antibodies","volume":"34","author":"DE PAIVA TM","year":"1992","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"ref8","first-page":"2774","article-title":"Endogenous hyaluronan in corneal disease","volume":"35","author":"FITZSIMMONS TD","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.biochi.2008.07.007","article-title":"Role of lactoferrin in the tear film","volume":"91","author":"FLANAGAN JL","year":"2009","journal-title":"Biochimie"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1098\/rspb.1922.0023","article-title":"On a Remarkable Bacteriolytic Element Found in Tissues and Secretions","volume":"93","author":"FLEMING A","year":"1922","journal-title":"Proceedings of the Royal Society of London. 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