{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:54:51Z","timestamp":1780595691659,"version":"3.54.1"},"reference-count":52,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"abstract":"<jats:p>ABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10\/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These findings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water.<\/jats:p>","DOI":"10.1590\/0001-3765201620150151","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T23:45:46Z","timestamp":1461800746000},"page":"635-641","source":"Crossref","is-referenced-by-count":13,"title":["Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools"],"prefix":"10.1590","volume":"88","author":[{"given":"RODRIGO","family":"STAGGEMEIER","sequence":"first","affiliation":[{"name":"Universidade Feevale, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"THALITA","family":"ARANTES","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KARIN S.","family":"CAUMO","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARILISE B.","family":"ROTT","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FERNANDO R.","family":"SPILKI","sequence":"additional","affiliation":[{"name":"Universidade Feevale, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2016,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1016\/j.watres.2009.01.025","article-title":"Analysis of Adenoviruses and Polyomaviruses quantified by PCR as indicators of water quality in source and drinking-water treatment plants","volume":"43","author":"Albinana-Gimenez N","year":"2009","journal-title":"Water Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1099\/00221287-140-6-1253","article-title":"Trojan horses of the 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