{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T01:40:20Z","timestamp":1768700420666,"version":"3.49.0"},"reference-count":53,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"abstract":"<jats:p>Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A\/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The infected mice showed local inflammation through the production of IFN-\u03b3 and TNF-\u03b1. Ureaplasma diversum infections in the reproductive tract of studied mice seemed to be associated with the production of pro-inflammatory cytokines in uterine parenchyma. The levels of TNF-\u03b1 of infected mice were dependent on the bacterial load of inoculated Ureaplasma. Uterine experimental infections by Ureaplasma diversum have not been mentioned yet and herein we presented the first report of an intrauterine infection model in mice.<\/jats:p>","DOI":"10.1590\/0001-3765201620150244","type":"journal-article","created":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T00:04:19Z","timestamp":1454544259000},"page":"643-652","source":"Crossref","is-referenced-by-count":11,"title":["Intra-uterine experimental infection by Ureaplasma diversum induces TNF-\u03b1 mediated womb inflammation in mice"],"prefix":"10.1590","volume":"88","author":[{"given":"Jamile R.","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00edcio F.A.A.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perc\u00edllia V.S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan\u00e9ia V.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00cdtalo S.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Timenetsky","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas M.","family":"Marques","sequence":"additional","affiliation":[{"name":"Universidade Federal da Bahia, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiana B.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Universidade 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M","year":"2012","journal-title":"PLoS One"},{"key":"ref7","first-page":"275","article-title":"Activation of murine macrophages and lymphocytes by Ureaplasma diversum","volume":"58","author":"Chelmonska-Soyta A","year":"1994","journal-title":"Can J Vet Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4792","DOI":"10.1128\/IAI.00522-10","article-title":"Interaction between pathogenic bacteria and intrauterine leukocytes triggers alternative molecular signaling cascades leading to labor in women","volume":"78","author":"Estrada-Gutierrez G","year":"2010","journal-title":"Infect Immun"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1203\/PDR.0b013e3181aabd66","article-title":"Surfactant Protein-A Limits Ureaplasma-Mediated Lung Inflammation in a Murine Pneumonia Model","volume":"66","author":"Famuyide ME","year":"2009","journal-title":"Pediatr Res 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Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1095\/biolreprod44.1.69","article-title":"The effects of soluble products of activated lymphocytes and macrophages on blastocyst implantation events in vitro","volume":"44","author":"Haimovici F","year":"1991","journal-title":"Biol Reprod"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1442-200X.2007.02391.x","article-title":"Certain type of chronic lung disease of newborns is associated with Ureaplasma urealyticum infection in utero","volume":"49","author":"Honma Y","year":"2007","journal-title":"Pediatr Int"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1477-7827-9-79","article-title":"Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow","volume":"9","author":"Jiemtaweeboon S","year":"2011","journal-title":"Reprod Biol Endocrinol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1128\/IAI.62.5.1528-1533.1994","article-title":"Ureaplasma diversum infection in vitro alters prostaglandin E2and prostaglandin F2a production by bovine endometrial cells without affecting cell viability","volume":"62","author":"Kim JJ","year":"1994","journal-title":"Infect Immun"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s13073-014-0107-1","article-title":"Complex host genetics influence the microbiome in inflammatory bowel disease","volume":"6","author":"Knights D","year":"2014","journal-title":"Genome Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s00005-013-0218-1","article-title":"The novel roles of neutrophils via opioid peptides: regulation of the estrous cycle and pain","volume":"61","author":"Kobayashi Y","year":"2013","journal-title":"Arch Immunol Ther Exp"},{"key":"ref19","first-page":"435","article-title":"Abortion due to Ureaplasma diversum","volume":"30","author":"Kreplin CM","year":"1989","journal-title":"Can Vet J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/jcpe.12226","article-title":"Role of cytokines in development of pre-eclampsia associated with periodontal disease-cohort study","volume":"41","author":"Kumar A","year":"2014","journal-title":"J Clin Periodontol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3906","DOI":"10.1128\/IAI.69.6.3906-3915.2001","article-title":"Ureaplasma urealyticum modulates endotoxin-induced cytokine release by human monocytes derived from preterm and term newborns and adults","volume":"69","author":"Manimtim WM","year":"2001","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.3748\/wjg.v19.i17.2591","article-title":"Tumor necrosis factor-\u03b1 inhibitor therapy and fetal risk: a systematic 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Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/01.qco.0000224819.42729.2e","article-title":"Antenatal infection and inflammation: what's new?","volume":"19","author":"Maxwell NC","year":"2006","journal-title":"Curr Opin Infect Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1093\/infdis\/jit101","article-title":"Mycoplasma genitalium infection activates cellular host defense and inflammation pathways in a 3-dimensional human endocervical epithelial cell model","volume":"207","author":"McGowin CL","year":"2013","journal-title":"J Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ijpara.2008.08.002","article-title":"The immunobiology of the innate response to Toxoplasma gondii","volume":"39","author":"Miller CM","year":"2009","journal-title":"Int J Parasitol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0749-0720(15)30533-8","article-title":"Ureaplasma diversum as a cause of reproductive disease in cattle","volume":"10","author":"Miller RB","year":"1994","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/j.1439-0442.1991.tb01062.x","article-title":"Concurrent conjunctivitis and placentitis in aborted bovine fetuses","volume":"38","author":"Murray RD","year":"1991","journal-title":"Zentralbl Veterinarmed A"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1093\/clinids\/17.Supplement_1.S144","article-title":"Leukocyte counts and colonization with Ureaplasma urealyticum in preterm neonates","volume":"17","author":"Ohlsson A","year":"1993","journal-title":"Clin Infect Dis"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s12223-013-0238-6","article-title":"Microbial diversity in uterus of healthy and metritic postpartum Holstein dairy cows","volume":"58","author":"Peng Y","year":"2013","journal-title":"Folia Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2334-9-9","article-title":"Different inflammatory responses are associated with Ureaplasma parvum-induced UTI and urolith formation","volume":"9","author":"Reyes L","year":"2009","journal-title":"BMC Infect Dis"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"e62746","DOI":"10.1371\/journal.pone.0062746","article-title":"Ureaplasma parvum serovar 3 multiple banded antigen size variation after chronic intra-amniotic infection\/colonization","volume":"8","author":"Robinson JW","year":"2013","journal-title":"PLoS 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A"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.bbrc.2009.02.112","article-title":"Regulation of the estrous cycle by neutrophil infiltration into the vagina","volume":"382","author":"Sasaki S","year":"2009","journal-title":"Biochem Biophys Res Commun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev.immunol.23.021704.115653","article-title":"How neutrophils kill microbes","volume":"23","author":"Segal AW","year":"2005","journal-title":"Annu Rev Immunol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"3253","DOI":"10.1128\/jb.175.11.3253-3258.1993","article-title":"Hydrolysis of urea by Ureaplasma urealyticum generates a transmembrane potential with resultant ATP synthesis","volume":"175","author":"Smith DG","year":"1993","journal-title":"J Bacteriol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1111\/j.1749-6632.1967.tb27697.x","article-title":"Bovine T-strain mycoplasma","volume":"143","author":"Taylor-Robinson D","year":"1967","journal-title":"Ann N Y Acad Sci"},{"key":"ref43","first-page":"693","article-title":"Pathological and microbiological studies on pneumonic lungs from Danish calves","volume":"46","author":"Tegtmeier C","year":"1999","journal-title":"Zentralbl Veterinarmed B"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1136\/vr.151.16.472","article-title":"Isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium","volume":"151","author":"Thomas A","year":"2002","journal-title":"Vet Rec"},{"key":"ref45","series-title":"Manual of Clinical Microbiology","author":"Versalovic J","year":"2011","edition":"10"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.clp.2009.12.003","article-title":"Ureaplasma species: role in diseases of prematurity","volume":"37","author":"Viscardi RM","year":"2010","journal-title":"Clin Perinatol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"F87","DOI":"10.1136\/archdischild-2012-303351","article-title":"Ureaplasma species: role in neonatal morbidities and outcomes","volume":"99","author":"Viscardi RM","year":"2014","journal-title":"Arch Dis Child"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/IAI.70.10.5721-5729.2002","article-title":"Characterization of a murine model of Ureaplasma urealyticum pneumonia","volume":"70","author":"Viscardi RM","year":"2002","journal-title":"Infect Immun"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"e44047","DOI":"10.1371\/journal.pone.0044047","article-title":"Host genetic background impacts disease outcome during intrauterine infection with Ureaplasma parvum","volume":"7","author":"Von Chamier M","year":"2012","journal-title":"PLoS 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