{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T06:39:00Z","timestamp":1770532740446,"version":"3.49.0"},"reference-count":65,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Acad. Bras. Ci\u00eanc."],"abstract":"<jats:p>ABSTRACT The present study was designed to investigate the antioxidant, antinociceptive and anti-inflammatory activities of the ethanol extract from Ageratum fastigiatum branches. Phytochemical screening and total phenol and flavonoid contents were determined. The antioxidant activity was assessed by 2,2-diphenyl-1-pycrilhydrazin (DPPH) and iron reducing power methods. The antinociceptive effect was evaluated using the acetic acid-induced writhing, formalin, hot plate and tail immersion assays; while the carrageenan-induced paw edema and pleurisy tests were performed to examine the anti-inflammatory activity against acute inflammation. The extract revealed the presence of flavonoids, tannins, coumarins, terpenes, sterols and saponins. Expressive levels of total phenols and flavonoids and a promising antioxidant effect were quantified. At the doses of 50, 100 and 200 mg\/kg, the extract inhibited the writhing, reduced both phases of paw licking time and increased the reaction time on the hot plate. In the tail immersion test, the extract (50, 100 and 200 mg\/kg) caused a significant inhibition of pain. In these doses, the paw edema, exudate volume and leucocyte mobilization were significantly reduced. These results suggest that A. fastigiatum can be an active source of substances with antioxidant, antinociceptive and anti-inflammatory activities, adding scientific support to the appropriate use in the Brazilian folk medicine.<\/jats:p>","DOI":"10.1590\/0001-3765201620150246","type":"journal-article","created":{"date-parts":[[2016,7,9]],"date-time":"2016-07-09T13:28:18Z","timestamp":1468070898000},"page":"1471-1484","source":"Crossref","is-referenced-by-count":3,"title":["Bioactivities of the ethanol extract from Ageratum fastigiatum branches: antioxidant, antinociceptive and anti-inflammatory"],"prefix":"10.1590","volume":"88","author":[{"given":"GLAUCIEMAR","family":"DEL-VECHIO-VIEIRA","sequence":"first","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BRUNA C.S.","family":"SANTOS","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIA SILVANA","family":"ALVES","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A\u00cdLSON L.A.","family":"ARA\u00daJO","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00c9LIA H.","family":"YAMAMOTO","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00cdRIAM A.O.","family":"PINTO","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIA AUXILIADORA C.","family":"KAPLAN","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ORLANDO V.","family":"SOUSA","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2016,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0006-8993(82)91282-3","article-title":"Brainstem lesions dissociate neural mechanisms of morphine analgesia in different kinds of pain","volume":"251","author":"Abbott FV","year":"1982","journal-title":"Brain Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/s11515-011-1123-9","article-title":"An overview of inflammation: mechanism and consequences.","volume":"6","author":"Ahmed AU","year":"2011","journal-title":"Front Biol"},{"key":"ref3","first-page":"13","article-title":"Inflammation, atherosclerosis, and coronary artery disease: PET\/CT for the evaluation of atherosclerosis and inflammation","volume":"8","author":"Alie N","year":"2014","journal-title":"Clin Med Insights Cardiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1023\/B:VEGE.0000046061.41915.d9","article-title":"Geographical distribution of Eupatorieae (Asteraceae) in South-eastern and South Brazilian Mountain Ranges","volume":"174","author":"Almeida AM","year":"2004","journal-title":"Plant Ecol"},{"key":"ref5","first-page":"74","article-title":"Influence of indomethacin and other anti-inflammatory drugs on mobilization and production of neutrophils: Studies with carrageenan-induced inflammation in rats","volume":"214","author":"Almeida AP","year":"1980","journal-title":"J Pharmacol Exp Ther"},{"key":"ref6","first-page":"1296","article-title":"Micropropagation of medicinally important plant species of family Asteraceae - a review","volume":"4","author":"Amim S","year":"2013","journal-title":"Int J Rec Sci Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/BF02026439","article-title":"Leucocyte migration and lysosomal enzymes release in rat carrageenin pleurisy","volume":"5","author":"Ammendola G","year":"1975","journal-title":"Agents Actions"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4103\/0970-5333.132845","article-title":"Ameliorative effect of ethyl pyruvate in neuropathic pain induced by chronic constriction injury of sciatic nerve","volume":"28","author":"Bansode VJ","year":"2014","journal-title":"Indian J Pain"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1016\/0031-9422(81)80061-1","article-title":"Labdane and eudesmane derivatives from Ageratum fastigiatum","volume":"20","author":"Bohlmann F","year":"1981","journal-title":"Phytochemistry"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/0031-9422(83)85036-5","article-title":"Daucanolide further farnesene derivatives from Ageratum fastigiatum","volume":"22","author":"Bohlmann F","year":"1983","journal-title":"Phytochemistry"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/path.1711160208","article-title":"Further studies on carrageenan-induced pleurisy in rats","volume":"116","author":"Capasso F","year":"1975","journal-title":"J Pathol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1476-5381.1968.tb00973.x","article-title":"The abdominal response and its suppression by analgesic drugs in the mouse","volume":"32","author":"Collier HO","year":"1968","journal-title":"Br J Pharmacol Chemother"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1016\/j.bbagen.2013.02.008","article-title":"Natural products: a continuing source of novel drug leads","author":"Cragg GM and Newman DJ.","year":"2013","journal-title":"Biochim Biophys Acta"},{"key":"ref14","first-page":"181","article-title":"Antinociceptive and antimicrobial activities of Ageratum fastigiatum (Gardn.) R. M. King et H. Hob. (Asteraceae)","volume":"88","author":"Del-Vechio G","year":"2007","journal-title":"Rev Bras Farm"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1590\/S0102-695X2008000500023","article-title":"Caracteriza\u00e7\u00e3o morfoanat\u00f4mica de Ageratum fastigiatum (Asteraceae)","volume":"18","author":"Del-Vechio-Vieira G","year":"2008","journal-title":"Rev Bras Farm acogn"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1590\/S1516-89132009000500008","article-title":"Analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum","volume":"52","author":"Del-Vechio-Vieira G","year":"2009","journal-title":"Braz Arch Biol Technol"},{"key":"ref17","first-page":"52","article-title":"Chemical composition and antimicrobial activity of the essential oils of Ageratum fastigiatum","volume":"3","author":"Del-Vechio-Vieira G","year":"2009","journal-title":"Rec Nat Prod"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0014-2999(80)90377-5","article-title":"Release of prostaglandins E and F in an algogenic reaction and its inhibition","volume":"61","author":"Deraedt R","year":"1980","journal-title":"Eur J Pharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.2042-7158.1972.tb08940.x","article-title":"Biological properties of carrageenan.","volume":"24","author":"Di Rosa M","year":"1972","journal-title":"J Pharm Pharmacol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/path.1711040103","article-title":"Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine","volume":"104","author":"Di Rosa M","year":"1971","journal-title":"J Pathol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/0014-2999(68)90103-9","article-title":"The mechanism of the inflammatory effect of carrageenan","volume":"4","author":"Di Rosa M and Sorrentino L","year":"1968","journal-title":"Eur J Pharmacol"},{"key":"ref22","first-page":"385","article-title":"Synthetic analgesics. II. Dithienylbutenyl-and dithienylbutylamines","volume":"107","author":"Eddy NB","year":"1953","journal-title":"J Pharmacol Exp Ther"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1093\/bjaceaccp\/mkt043","article-title":"Complex regional pain syndrome: recent updates.","volume":"14","author":"Ganty P","year":"2014","journal-title":"Contin Educ Anaesth Crit Care Pain"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2174\/138620706777698481","article-title":"Antioxidants and inflammatory disease: synthetic and natural antioxidants with anti-inflammatory activity","volume":"9","author":"Geronikaki AA","year":"2006","journal-title":"Comb Chem High Throughput Screen"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1590\/S0102-695X2011005000151","article-title":"Contribution for the phytochemical studies of Ageratum fastigiatum","volume":"21","author":"Gon\u00e7alves LD","year":"2011","journal-title":"Rev Bras Farm acogn"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fphys.2014.00352","article-title":"Reactive oxygen species at the crossroads of inflammasome and inflammation","volume":"5","author":"Harijith A","year":"2014","journal-title":"Front Physiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0304-3959(87)90088-1","article-title":"The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain","volume":"30","author":"Hunskaar S","year":"1987","journal-title":"Pain"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s11154-014-9301-0","article-title":"Adipocyte dysfunction, inflammation and metabolic syndrome","volume":"15","author":"Kl\u00f6ting N","year":"2014","journal-title":"Rev Endocr Metab Dosord"},{"key":"ref29","first-page":"272","article-title":"Anti-inflammatory activity of herbal plants: A review","volume":"2","author":"Kumar S","year":"2013","journal-title":"Int J Adv Pharm Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/162750","article-title":"Chemistry and biological activities of flavonoids: An overview","author":"Kumar S and Pandey AK.","year":"2013","journal-title":"Sci World J"},{"key":"ref31","first-page":"121","article-title":"Anti-inflammatory agents from plants - Part III","volume":"5","author":"Lehra KS","year":"2014","journal-title":"Indian J Nat Prod Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/BF01234480","article-title":"A new approach to practical acute toxicity testing","volume":"54","author":"Lorke D.","year":"1983","journal-title":"Arch Toxicol"},{"key":"ref33","first-page":"99","article-title":"A simplified method of evaluating dose-effect experiments","volume":"96","author":"Litchfield JT","year":"1949","journal-title":"J Pharmacol Exp Ther"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/ptr.687","article-title":"Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method","volume":"15","author":"Mensor LL","year":"2001","journal-title":"Phytother Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0014-2999(82)90123-6","article-title":"Comparison of the anti-inflammatory effects of dexamethasone, indomethacin and BW755C on carrageenin-induced pleurisy in rats","volume":"77","author":"Miyasaka K","year":"1982","journal-title":"Eur J Pharmacol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/sj.bjp.0702866","article-title":"Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation","volume":"128","author":"Nantel F","year":"1999","journal-title":"Br J Pharmacol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1097\/00000539-200107000-00039","article-title":"The antinociceptive effect of the combination of spinal morphine with systemic morphine or buprenorphine","volume":"93","author":"Nemirovsky A","year":"2011","journal-title":"Anesth Analg"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.3390\/ijms16011232","article-title":"Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema","volume":"16","author":"Oh YC","year":"2015","journal-title":"Int J Mol Sci"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0367-326X(01)00364-1","article-title":"Ageratum conyzoides L. (Asteraceae)","volume":"73","author":"Okunade AL.","year":"2002","journal-title":"Fitoterapia"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on product of browning reaction-antioxidative activities of products of browning reaction prepared from glucosamine.","volume":"44","author":"Oyaizu M.","year":"1986","journal-title":"Jpn J Nutr"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s00005-007-0039-1","article-title":"Terpenes: substances useful in human healthcare.","volume":"55","author":"Paduch R","year":"2007","journal-title":"Arch Immunol Ther Exp"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0160-5402(89)90019-3","article-title":"Tail immersion test for the evaluation of a nociceptive reaction in mice: methodological considerations","volume":"21","author":"Ramabadran K","year":"1989","journal-title":"J Pharmacol Methods"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S1360-1385(97)01018-2","article-title":"Antioxidant properties of phenolic compounds","volume":"2","author":"Rice-Evans C","year":"1997","journal-title":"Trends Plant Sci"},{"key":"ref44","first-page":"31","article-title":"Evaluation of anti-inflammatory and antinociceptive activity and isolation of two new alkaloids from leaves extract of Tabernaemontana sananho","volume":"7","author":"Rohini RM and Mahesh D.","year":"2015","journal-title":"J Chem Pharm Res"},{"key":"ref45","first-page":"304","article-title":"Free radicals after painful stimulation are influenced by antioxidants and analgesics","volume":"24","author":"Rokyta R","year":"2003","journal-title":"Neuroendocrinol Lett"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0304-3959(90)91167-H","article-title":"The formalin test in mice: effect of formalin concentration.","volume":"42","author":"Rosland JH","year":"1990","journal-title":"Pain"},{"key":"ref47","first-page":"1","article-title":"In vivo studies on spinal opiate receptor systems mediating antinociception. II. Pharmacological profiles suggesting a differential association of mu, delta and kappa receptors with visceral chemical and cutaneous thermal stimuli in the rat","volume":"228","author":"Schmauss C","year":"1984","journal-title":"J Pharmacol Exp Ther"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1146\/annurev.bioeng.8.061505.095708","article-title":"Analysis of inflammation","volume":"8","author":"Schmid-Sch\u00f6nbein GW.","year":"2006","journal-title":"Annu Rev Biomed Eng"},{"key":"ref49","first-page":"91","article-title":"Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect","volume":"3","author":"Sen S","year":"2010","journal-title":"Int J Pharm Sci Rev Res"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1017\/S002966511000162X","article-title":"Flavonoids as anti-inflammatory agents","volume":"69","author":"Serafini M","year":"2010","journal-title":"Proc Nutr Soc"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3923\/rjmp.2011.101.115","article-title":"A review on medicinal plants as a source of anti-inflammatory agents","volume":"5","author":"Shah BN","year":"2011","journal-title":"Res J Med Plant"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0304-3959(89)90222-4","article-title":"Modified formalin test: characteristic biphasic pain response.","volume":"38","author":"Shibata M","year":"1989","journal-title":"Pain"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1055\/s-0030-1267855","article-title":"Pharmacology of nonsteroidal antiinflammatory drugs and opioids","volume":"27","author":"Slater D","year":"2010","journal-title":"Semin Intervent Radiol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"650","DOI":"10.3390\/ph6050650","article-title":"Natural products as a source for new anti-inflammatory and analgesic compounds through the inhibition of purinergic P2X receptors","volume":"6","author":"Soares-Bezerra RJ","year":"2013","journal-title":"Pharmaceuticals"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1590\/S1516-93322008000400015","article-title":"Valida\u00e7\u00e3o de metodologia espectrofotom\u00e9trica para quantifica\u00e7\u00e3o dos flavon\u00f3ides de Bauhinia cheilantha (Bongard) Steudel","volume":"44","author":"Tjsp Sobrinho","year":"2008","journal-title":"Rev Bras Ci\u00eanc Farm"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1590\/S0100-40422007000200021","article-title":"Fen\u00f3is totais e atividade antioxidante de cinco plantas medicinais","volume":"30","author":"Cmm Sousa","year":"2007","journal-title":"Qu\u00edm Nova"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/bcpt.12221","article-title":"Structure-activity relationship of terpenes with anti-inflammatory profile - a systematic review","volume":"115","author":"Souza MT","year":"2014","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"106","DOI":"10.11648\/j.ajhr.20140204.11","article-title":"Side effects of non-steroidal anti-inflammatory drugs: The experience of patients with musculoskeletal disorders","volume":"2","author":"Teslim OA","year":"2014","journal-title":"Am J Health Res"},{"key":"ref59","first-page":"98","article-title":"Phytochemical screening and extraction: A review","volume":"1","author":"Tiwari P","year":"2011","journal-title":"Int Pharm Sci"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0304-3959(92)90003-T","article-title":"The formalin test: an evaluation of the method","volume":"51","author":"Tj\u00f8lsen A","year":"1992","journal-title":"Pain"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1021\/tx9600964","article-title":"A quantum chemical explanation of the antioxidant activity of flavonoids","volume":"9","author":"Sabe Van Acker","year":"1996","journal-title":"Chem Res Toxicol"},{"key":"ref62","first-page":"96","article-title":"Biphasic development of carrageenin edema in rats.","volume":"166","author":"Vinegar R","year":"1969","journal-title":"J Pharmacol Exp Ther"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"711","DOI":"10.3181\/00379727-143-37397","article-title":"Some quantitative temporal characteristics of carrageenin-induced pleurisy in the rat","volume":"143","author":"Vinegar R","year":"1973","journal-title":"Proc Soc Exp Biol Med"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3181\/00379727-111-27849","article-title":"Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs","volume":"111","author":"Winter CA","year":"1962","journal-title":"Proc Soc Exp Biol Med"},{"key":"ref65","first-page":"411","article-title":"Studies on the direct spinal action of narcotics in the production of analgesia in the rat.","volume":"202","author":"Yaksh TL","year":"1977","journal-title":"J Pharmacol Exp Ther"}],"container-title":["Anais da Academia Brasileira de Ci\u00eancias"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/aabc\/v88n3\/0001-3765-aabc-201620150246.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T22:32:40Z","timestamp":1656801160000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0001-37652016000401471&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,11]]},"references-count":65,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,9]]}},"alternative-id":["S0001-37652016000401471"],"URL":"https:\/\/doi.org\/10.1590\/0001-3765201620150246","relation":{},"ISSN":["1678-2690","0001-3765"],"issn-type":[{"value":"1678-2690","type":"electronic"},{"value":"0001-3765","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7,11]]}}}