{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:30:52Z","timestamp":1790375452873,"version":"4.1.0"},"reference-count":27,"publisher":"FapUNIFESP (SciELO)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Neuro-Psiquiatr."],"abstract":"<jats:p>ABSTRACT Neuropathic pain is a chronic pain condition caused by damage or dysfunction of the central or peripheral nervous system. Electroacupuncture (EA) has an antinociceptive effect on neuropathic pain, which is partially due to inhibiting astrocyte activation in the spinal cord. We found that an intrathecal injection of 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), a selective adenosine A1 receptor antagonist, reversed the antinociceptive effects of EA in a chronic constriction injury-induced neuropathic pain model. The expression of GFAP in L4-L6 spinal cord was significantly upgraded, while DPCPX suppressed the effect of the EA-mediating inhibition of astrocyte activation, as well as wiping out the EA-induced suppression of cytokine content (TNF-\u03b1). These results indicated that the adenosine A1 receptor is involved in EA actions during neuropathic pain through suppressing astrocyte activation as well as TNF-\u03b1 upregulation of EA, giving enlightenment to the mechanisms of acupuncture analgesia and development of therapeutic targets for neuropathic pain.<\/jats:p>","DOI":"10.1590\/0004-282x20180128","type":"journal-article","created":{"date-parts":[[2018,12,15]],"date-time":"2018-12-15T13:42:55Z","timestamp":1544881375000},"page":"736-742","source":"Crossref","is-referenced-by-count":31,"title":["Involvement of adenosine A1 receptor in electroacupuncture-mediated inhibition of astrocyte activation during neuropathic pain"],"prefix":"10.1590","volume":"76","author":[{"given":"Mingxiao","family":"Zhang","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinxue","family":"Dai","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Liang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangdong","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunyuan","family":"Han","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luping","family":"Huang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlu","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2018,11]]},"reference":[{"issue":"252","key":"ref1","article-title":"Cell signaling and the genesis of neuropathic pain","volume":"2004","author":"Ji RR","year":"2004","journal-title":"Sci STKE"},{"issue":"14","key":"ref2","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.2165\/00023210-200317140-00003","article-title":"Pharmacological management of neuropathic pain","volume":"17","author":"McCleane G","year":"2003","journal-title":"CNS Drugs"},{"issue":"S2 Suppl 2","key":"ref3","doi-asserted-by":"crossref","first-page":"S183","DOI":"10.1007\/s10072-006-0598-7","article-title":"Medications for neuropathic pain: current trends","volume":"27","author":"Colombo B","year":"2006","journal-title":"Neurol Sci"},{"key":"ref4","series-title":"Acupuncture: review and analysis of reports on controlled clinical trials","year":"2003"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0031-6997(24)01511-4","article-title":"International Union of Pharmacology. XXV. Nomenclature and classification of adenosine receptors","volume":"53","author":"Fredholm BB","year":"2001","journal-title":"Pharmacol Rev"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1111\/j.1471-4159.1979.tb05236.x","article-title":"Adenosine regulates via two different types of receptors, the accumulation of cyclic AMP in cultured brain cells","volume":"33","author":"Calker D","year":"1979","journal-title":"J Neurochem"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1046\/j.1471-4159.1996.66041426.x","article-title":"Adenosine A2b receptors mediate an increase in interleukin (IL)-6 mRNA and IL-6 protein synthesis in human astroglioma cells","volume":"66","author":"Fiebich BL","year":"1996","journal-title":"J Neurochem"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1046\/j.1471-4159.1997.69031145.x","article-title":"Stimulation of interleukin-6 secretion and gene transcription in primary astrocytes by adenosine","volume":"69","author":"Schwaninger M","year":"1997","journal-title":"J Neurochem"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/jnr.490390507","article-title":"Effects of exogenous ATP and related analogues on the proliferation rate of dissociated primary cultures of rat astrocytes","volume":"39","author":"Ciccarelli R","year":"1994","journal-title":"J Neurosci Res"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0304-3959(88)90209-6","article-title":"A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man","volume":"33","author":"Bennett GJ","year":"1988","journal-title":"Pain"},{"issue":"5","key":"ref11","first-page":"363","article-title":"[Effect of acupuncture at \u201cZusanli\u201d (ST 36 and \u201cTaichong\u201d (LR 3) on gastrointestinal hormone levels in rats with diarrhea type irritable bowel syndrome]","volume":"37","author":"Liu MR","year":"2012","journal-title":"Zhen Ci Yan Jiu"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.brainresbull.2007.07.027","article-title":"Evidence for suppression of electroacupuncture on spinal glial activation and behavioral hypersensitivity in a rat model of monoarthritis","volume":"75","author":"Sun S","year":"2008","journal-title":"Brain Res Bull"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0304-3959(88)90026-7","article-title":"A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia","volume":"32","author":"Hargreaves K","year":"1988","journal-title":"Pain"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.pharmthera.2010.01.002","article-title":"Chemokines, neuronal-glial interactions, and central processing of neuropathic pain","volume":"126","author":"Gao YJ","year":"2010","journal-title":"Pharmacol Ther"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1177\/1073858409360822","article-title":"Glial cells and chronic pain","volume":"16","author":"Gosselin RD","year":"2010","journal-title":"Neuroscientist"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1016\/j.metabol.2010.07.011","article-title":"Spinal glia and chronic pain","volume":"59","author":"O'Callaghan JP","year":"2010","journal-title":"Metabolism"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.molmed.2006.12.005","article-title":"The tripartite synapse: roles for gliotransmission in health and disease","volume":"13","author":"Halassa MM","year":"2007","journal-title":"Trends Mol Med"},{"issue":"42","key":"ref18","doi-asserted-by":"crossref","first-page":"10482","DOI":"10.1523\/JNEUROSCI.3593-08.2008","article-title":"Supraspinal glial-neuronal interactions contribute 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