{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:37:33Z","timestamp":1789767453887,"version":"4.0.1"},"reference-count":39,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Neuro-Psiquiatr."],"abstract":"<jats:p>ABSTRACT This study evaluated the operational characteristics of the multiplex polymerase chain reaction (PCR) for cerebrospinal fluid (CSF) from patients with cellular and biochemical characteristics of acute bacterial meningitis and positive or negative CSF cultures. Methods: Multiplex PCR was performed for 36 CSF samples: culture-proven acute bacterial meningitis (n = 7), culture-negative acute bacterial meningitis (n = 17), lymphocytic meningitis (n = 8), and normal CSF (n = 4). The operational characteristics of multiplex PCR were evaluated with definite and probable bacterial meningitis, using culture positive, cytological and biochemical CSF characteristics as the gold standard. Results: Multiplex PCR for CSF was efficient in the group with CSF cellular and biochemical characteristics of acute bacterial meningitis but with a negative CSF culture. This group demonstrated high specificity, positive predictive value, and efficiency. Conclusions: Multiplex PCR for CSF can improve the speed and accuracy of acute bacterial meningitis diagnosis in a clinical setting as a complement to classical immunological and bacteriological assays in CSF. It is also useful for CSF culture-negative acute bacterial meningitis.<\/jats:p>","DOI":"10.1590\/0004-282x20190028","type":"journal-article","created":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T19:30:09Z","timestamp":1557603009000},"page":"224-231","source":"Crossref","is-referenced-by-count":14,"title":["Validation of multiplex PCR for the diagnosis of acute bacterial meningitis in culture negative cerebrospinal fluid"],"prefix":"10.1590","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5690-105X","authenticated-orcid":false,"given":"S\u00e9rgio Monteiro","family":"de Almeida","sequence":"first","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil; Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libera Maria","family":"Dalla Costa","sequence":"additional","affiliation":[{"name":"Faculdades Pequeno Pr\u00edncipe, Brasil; Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Siebra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central do Estado do Paran\u00e1, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavinia Nery Villa Stangler","family":"Arend","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central do Estado do Paran\u00e1, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keite da Silva","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil; Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2019,4]]},"reference":[{"issue":"10063","key":"ref1","doi-asserted-by":"crossref","first-page":"P3036","DOI":"10.1016\/S0140-6736(16)30654-7","article-title":"Acute bacterial meningitis in adults","volume":"388","author":"McGill 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