{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T17:07:20Z","timestamp":1770224840637,"version":"3.49.0"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"10","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Neuro-Psiquiatr."],"abstract":"<jats:p>ABSTRACT This study aimed to analyze the cerebellum of rats submitted to an experimental focal cerebral ischemia, by middle cerebral artery occlusion for 90 minutes, followed by reperfusion for 48 hours, associated with an alcoholism model.  Methods  Fifty adult Wistar rats were used, subdivided into five experimental groups: control group (C): animals submitted to anesthesia only; sham group (S): animals submitted to complete simulation of the surgical procedure; ischemic group (I): animals submitted to focal cerebral ischemia for 90 minutes followed by reperfusion for 48 hours; alcoholic group (A): animals that received daily absolute ethanol diluted 20% in water for four weeks; and, ischemic and alcoholic group (I + A): animals receiving the same treatment as group A and, after four weeks, submitted to focal cerebral ischemia for 90 minutes, followed by reperfusion for 48 hours. The cerebellum samples were collected and immunohistochemical analysis of Caspase-9 protein and serum analysis by RT-PCR of microRNAs miR-21, miR-126 and miR155 were performed.  Results  The expression of Caspase-9 was higher in groups I, A and I + A. In the microRNAs analyses, miR-126 was higher in groups A and I + A, miR-155 was higher in groups I and I + A.  Conclusions  We conclude that apoptosis occurs in the cerebellar cortex, even if it is distant from the ischemic focus, and that microRNAs 126 and 155 show a correlation with cellular apoptosis in ischemic rats and those submitted to the chronic alcohol model.<\/jats:p>","DOI":"10.1590\/0004-282x20190126","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T15:52:42Z","timestamp":1571845962000},"page":"689-695","source":"Crossref","is-referenced-by-count":10,"title":["Analysis of Caspase-9 protein and microRNAs miR-21, miR-126 and miR-155 related to the apoptosis mechanism in the cerebellum of rats submitted to focal cerebral ischemia associated with an alcoholism model"],"prefix":"10.1590","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5213-8525","authenticated-orcid":false,"given":"Jairo Pinheiro da","family":"SILVA","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0046-7300","authenticated-orcid":false,"given":"Fermino Sanches","family":"LIZARTE NETO","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7872-8331","authenticated-orcid":false,"given":"Mucio Luiz de Assis","family":"CIRINO","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2325-2844","authenticated-orcid":false,"given":"Camila Albuquerque Melo de","family":"CARVALHO","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7055-0564","authenticated-orcid":false,"given":"Carlos Gilberto","family":"CARLOTTI JR","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0222-6770","authenticated-orcid":false,"given":"Benedicto Oscar","family":"COLLI","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1886-4988","authenticated-orcid":false,"given":"Daniela Pretti da Cunha","family":"TIRAPELLI","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2251-1106","authenticated-orcid":false,"given":"Lu\u00eds Fernando","family":"TIRAPELLI","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2019,10]]},"reference":[{"issue":"1","key":"ref1","first-page":"1","article-title":"Isquemia cerebral: mecanismos fisiopatol\u00f3gicos y oportunidades terap\u00e9uticas","volume":"2","author":"Campos-Mojena R","year":"2016","journal-title":"Rev Cien Farmac Aliment"},{"issue":"1","key":"ref2","first-page":"56","article-title":"Mortality Trend Due to Cerebrovascular Accident in the City of Maring\u00e1, Paran\u00e1 between the Years of 2005 to 2015","volume":"31","author":"Sard\u00e1 A","year":"2018","journal-title":"Int JCardiovasc Sci"},{"key":"ref3","first-page":"e28","article-title":"Heart disease and stroke statistics: 2014 update: A report from the American Heart Association","volume":"129","author":"Go AS","year":"2014","journal-title":"Circulation"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1159\/000489241","article-title":"Current Mechanistic Concepts in Ischemia and Reperfusion Injury","volume":"46","author":"Wu MY","year":"2018","journal-title":"Cell Physiol Biochem"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/498232","article-title":"MicroRNA Signature in alcoholic liver disease","volume":"2012","author":"Bala S","year":"2012","journal-title":"Int J Hepatol"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1134\/S0026893317040057","article-title":"The effects of chronic alcoholization on the expression of brain-derived neurotrophic factor and its receptors in the brains of mice genetically predisposed to depressive-like behavior","volume":"51","author":"Bazovkina D. V","year":"2017","journal-title":"Mol Biol"},{"key":"ref7","series-title":"Translational research in traumatic brain injury","volume-title":"Genetic influences in traumatic brain injury","author":"Bennett ER","year":"2016"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/jcmm.12223","article-title":"The functional role of microRNAs in alcoholic liver injury","volume":"18","author":"McDaniel K","year":"2014","journal-title":"J Cell Mol Med"},{"issue":"2","key":"ref9","first-page":"105","article-title":"MicroRNAs: biog\u00eanese, fun\u00e7\u00f5es e seu papel potencial na carcinog\u00eanese oral","volume":"9","author":"Aguiar BA","year":"2010","journal-title":"Odontol Clin Cient"},{"key":"ref10","first-page":"629","author":"Carvalho CAM","year":"2016"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0301-0082(98)00032-X","article-title":"Chronic ethanol consumption: from neuroadaptation to neurodegeneration","volume":"56","author":"Fadda F","year":"1998","journal-title":"Prog Neurobiol"},{"issue":"12","key":"ref12","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1177\/0963689718773361","article-title":"The roles of MicroRNAs in stroke: possible therapeutic targets","volume":"27","author":"Xu W","year":"2018","journal-title":"Cell Transplant"},{"issue":"2B","key":"ref13","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1590\/S0004-282X2001000300011","article-title":"Avalia\u00e7\u00e3o da isquemia cerebral pela respira\u00e7\u00e3o mitocondrial: Modelo experimental","volume":"59","author":"Carlotti CG","year":"2001","journal-title":"Arq Neuropsiquiatr"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1055\/s-0038-1626497","article-title":"Infarto cerebral hemisf\u00e9rico : algoritmo de tratamento baseado em evid\u00eancia","volume":"30","author":"Campos WK","year":"2011","journal-title":"Arq Bras Neurocir"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/5050469","article-title":"Neuroprotective effect of DAHP via antiapoptosis in cerebral ischemia","author":"Qin Y","year":"2018","journal-title":"Behav Neurol"},{"issue":"12","key":"ref16","first-page":"8322","article-title":"Autophagy, a process within reperfusion injury: an update","volume":"7","author":"Thapalia BA","year":"2014","journal-title":"Int J Clin Exp Pathol"},{"issue":"25","key":"ref17","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.3748\/wjg.v24.i25.2661","article-title":"Apoptosis and non-alcoholic fatty liver diseases","volume":"24","author":"Kanda T","year":"2018","journal-title":"World J Gastroenterol"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/01616412.2016.1251710","article-title":"Electrical stimulation of cerebellar fastigial nucleus protects against cerebral ischemic injury by PPAR\u03b3 upregulation","volume":"39","author":"Liu B","year":"2017","journal-title":"Neurol Res"},{"issue":"12","key":"ref19","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1038\/jcbfm.2013.157","article-title":"Inhibition of microRNA-181 reduces forebrain ischemia-induced neuronal loss","volume":"33","author":"Moon J. M.","year":"2013","journal-title":"J Cereb Blood Flow Metab"},{"issue":"8","key":"ref20","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1111\/cns.12288","article-title":"Electrical stimulation of cerebellar fastigial nucleus: mechanism of neuroprotection and prospects for clinical application against cerebral ischemia","volume":"20","author":"Wang J","year":"2014","journal-title":"CNS Neurosci Ther"},{"issue":"20","key":"ref21","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.1101\/gad.1126903","article-title":"Ways of dying: multiple pathways to apoptosis","volume":"17","author":"Adams JM","year":"2003","journal-title":"Genes Dev"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jhep.2018.02.005","article-title":"Apoptosis of enterocytes and nitration of junctional complex proteins promote alcohol-induced gut leakiness and liver injury","volume":"69","author":"Cho YE","year":"2018","journal-title":"J Hepatol"},{"issue":"6","key":"ref23","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1002\/hep.22256","article-title":"MicroRNA profiling in hepatocellular tumors is associated with clinical features and oncogene\/tumor suppressor gene mutations","volume":"47","author":"Ladeiro Y","year":"2008","journal-title":"Hepatology"},{"issue":"10","key":"ref24","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1080\/01616412.2018.1488654","article-title":"MiR-125b blocks Bax\/Cytochrome C\/Caspase-3 apoptotic signaling pathway in rat models of cerebral ischemia-reperfusion injury by targeting p53","volume":"40","author":"Xie YL","year":"2018","journal-title":"Neurol Res"}],"container-title":["Arquivos de Neuro-Psiquiatria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/anp\/v77n10\/0004-282X-anp-77-10-0689.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T18:16:17Z","timestamp":1581444977000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0004-282X2019001000689&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":24,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["S0004-282X2019001000689"],"URL":"https:\/\/doi.org\/10.1590\/0004-282x20190126","relation":{},"ISSN":["1678-4227","0004-282X"],"issn-type":[{"value":"1678-4227","type":"electronic"},{"value":"0004-282X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]}}}