{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T01:47:29Z","timestamp":1773712049981,"version":"3.50.1"},"reference-count":18,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"DOI":"10.1590\/0074-02760150070","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T19:58:27Z","timestamp":1433447907000},"page":"485-491","source":"Crossref","is-referenced-by-count":13,"title":["Molecular characterisation and disease severity of leptospirosis in Sri Lanka"],"prefix":"10.1590","volume":"110","author":[{"given":"Kanchana Kumari","family":"Bandara","sequence":"first","affiliation":[{"name":"University of Sri Jayewardenepura,  Sri Lanka; General Sir John Kotelawala Defense University,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjula","family":"Weerasekera","sequence":"additional","affiliation":[{"name":"University of Sri Jayewardenepura,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinthika P","family":"Gunasekara","sequence":"additional","affiliation":[{"name":"University of Sri Jayewardenepura,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilantha","family":"Ranasinghe","sequence":"additional","affiliation":[{"name":"Base Hospital,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chamil","family":"Marasinghe","sequence":"additional","affiliation":[{"name":"University of Sri Jayewardenepura,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neluka","family":"Fernando","sequence":"additional","affiliation":[{"name":"University of Sri Jayewardenepura,  Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002626","article-title":"Regional differences of leptospirosis in Sri Lanka: observations from a flood-associated outbreak in 2011","volume":"8","author":"Agampodi SB","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1093\/cid\/cis035","article-title":"Utility of quantitative polymerase chain reaction in leptospirosis diagnosis: association of level of leptospiremia and clinical manifestations in Sri Lanka","volume":"54","author":"Agampodi SB","year":"2012","journal-title":"Clin Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"184","DOI":"10.4269\/ajtmh.2012.12-0526","article-title":"Utility and limitations of direct multi-locus sequence typing on qPCR-positive blood to determine infecting Leptospira strain","volume":"88","author":"Agampodi SB","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"471","DOI":"10.4269\/ajtmh.2011.11-0276","article-title":"Leptospirosis outbreak in Sri Lanka in 2008: lessons for assessing the global burden of disease","volume":"85","author":"Agampodi SB","year":"2011","journal-title":"Am J Trop Med Hyg"},{"key":"ref5","author":"Bandara K","year":"2014"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2334-11-338","article-title":"Molecular detection and speciation of pathogenic Leptospira spp in blood from patients with culture-negative leptospirosis","volume":"11","author":"Boonsilp S","year":"2011","journal-title":"BMC Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/00207713-49-2-839","article-title":"Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies","volume":"49","author":"Brenner DJ","year":"1999","journal-title":"Int J Syst Bacteriol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/tbed.12014","article-title":"Carrier status of leptospirosis among cattle in Sri Lanka: a zoonotic threat to public health","volume":"61","author":"Gamage CD","year":"2014","journal-title":"Transbound Emerg Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1348-0421.2001.tb02649.x","article-title":"flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp","volume":"45","author":"Kawabata H","year":"2001","journal-title":"Microbiol Immunol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1099\/jmm.0.011528-0","article-title":"Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan","volume":"58","author":"Koizumi N","year":"2009","journal-title":"J Med Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett PN","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.sjbs.2011.11.005","article-title":"Rapid diagnosis of leptospirosis in patients with different clinical manifestations by 16S rRNA gene based nested PCR","volume":"19","author":"Natarajaseenivasan K","year":"2012","journal-title":"Saudi J Biol Sci"},{"key":"ref13","first-page":"943","article-title":"Leptospira infection at the University of Peradeniya Teaching Hospital, Sri Lanka: clinical and laboratory investigations","volume":"43","author":"Nwafor-Okoli C","year":"2012","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"ref14","first-page":"64","article-title":"Seroprevalece of leptospirosis in South Gujarat region by evaluating the two rapid commercial diagnostic kits against the MAT test for detection of antibodies to Leptospira interrogans","volume":"2","author":"Panwala T","year":"2011","journal-title":"National Journal of Community Medicine"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2334-2-13","article-title":"A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp","volume":"2","author":"Smythe LD","year":"2002","journal-title":"BMC Infect Dis"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2334-9-147","article-title":"Leptospirosis in the Asia Pacific Region","volume":"9","author":"Victoriano AF","year":"2009","journal-title":"BMC Infect Dis"},{"key":"ref17","series-title":"Report of the first meeting of the leptospirosis burden epidemiology reference group","year":"2010"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"899","DOI":"10.4269\/ajtmh.2010.09-0721","article-title":"Molecular epidemiology of leptospirosis in northern Iran by nested polymerase chain reaction\/restriction fragment length polymorphism and sequencing methods","volume":"82","author":"Zakeri S","year":"2010","journal-title":"Am J Trop Med Hyg"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/mioc\/v110n4\/0074-0276-mioc-0074-02760150070.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:04:49Z","timestamp":1520294689000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762015000400485&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,9]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,6]]}},"alternative-id":["S0074-02762015000400485"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760150070","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2015,6,9]]}}}