{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T05:55:43Z","timestamp":1771566943551,"version":"3.50.1"},"reference-count":54,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1590\/0074-02760160435","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T13:56:37Z","timestamp":1496670997000},"page":"403-410","source":"Crossref","is-referenced-by-count":23,"title":["Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil"],"prefix":"10.1590","volume":"112","author":[{"given":"Sandra","family":"Yamashiro","sequence":"first","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vagner Ricardo da Silva","family":"Fiuza","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c2ngela Therezinha Lauand Sampaio","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilson","family":"Branco","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Em\u00edlio","family":"Levy","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel Cristina Vidal Siqueira de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina Maura Bueno","family":"Franco","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"7","key":"ref1","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1590\/S1807-59322011000700008","article-title":"Intestinal microsporidiosis: a hidden risk in rheumatic disease patients undergoing anti-tumor necrosis fator therapy combined with disease-modifying anti-rheumatic drugs?","volume":"66","author":"Aikawa NE","year":"2011","journal-title":"Clinics"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2166\/wh.2012.204","article-title":"Survey and genetic characterization of wastewater in Tunisia for Cryptospori- dium spp., Giardia duodenalis, Enterocytozoon bieneusi, Cyclospora cayetanensis and Eimeria spp","volume":"10","author":"Ayed LB","year":"2012","journal-title":"J Water Health"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0036-46652000000600001","article-title":"Clinical and diagnostic aspects of intestinal microsporidiosis in HIV-infected patients with chronic diarrhea in Rio de Janeiro, Brazil","volume":"42","author":"Brasil P","year":"2000","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1590\/S0036-46651998000400002","article-title":"A 3-year follow-up of a Brazilian AIDS patient with protracted diarrhea caused by Enterocytozoon bieneusi","volume":"40","author":"Brasil P","year":"1998","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1590\/S0036-46651996000200003","article-title":"Intestinal microsporidiosis in HIV-positive patients with chronic unexplained diarrhea in RJ, Brasil: diagnosis, clinical presentation and follow-up","volume":"38","author":"Brasil P","year":"1996","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1128\/AEM.68.5.2595-2599.2002","article-title":"Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughterhouse in Massachusetts","volume":"68","author":"Buckholt MA","year":"2002","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"89","DOI":"10.2166\/wst.2006.453","article-title":"Evaluation of activated sludge treatment and the efficiency of the disinfection of Giardia species cysts and Cryptosporidium oocysts by UV at a sludge treatment plant in Campinas, Southeast Brazil","volume":"54","author":"Cantusio Neto R","year":"2006","journal-title":"Water Sci Technol"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s00436-011-2291-x","article-title":"Municipal wastewater treatment plants as removal systems and environmental sources of human-virulent microsporidian spores","volume":"109","author":"Cheng H-W A","year":"2011","journal-title":"Parasitol Res"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.bjid.2013.07.004","article-title":"Intestinal microsporidiosis in a reference center. Letter to the editor","volume":"17","author":"Costa LNG","year":"2013","journal-title":"Braz J Infect Dis"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1086\/315112","article-title":"Waterborne outbreak of intestinal microsporidiosis in persons with and without human immunodeficiency virus infection","volume":"180","author":"Cotte L","year":"1999","journal-title":"J Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.vetpar.2015.12.019","article-title":"Widespread presence of human-pathogenic Enterocytozoon bieneusi genotypes in chickens","volume":"217","author":"Cunha MJR","year":"2016","journal-title":"Vet Parasitol"},{"issue":"6","key":"ref12","first-page":"560","article-title":"Laboratory diagnosis of opportunistic intestinal parasites with emphasis on human microsporidiosis, in Goi\u00e2nia\/GO","volume":"39","author":"de Souza Jr ES","year":"2006","journal-title":"Rev Soc Bras Med Trop"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1017\/S095026881100077X","article-title":"First reported foodborne outbreak associated with microsporidia, Sweden, October 2009","volume":"140","author":"Decraene V","year":"2012","journal-title":"Epidemiol Infect"},{"issue":"1-2","key":"ref14","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetpar.2004.09.006","article-title":"Epidemiology of microsporidiosis: sources and modes of transmission","volume":"126","author":"Didier ES","year":"2004","journal-title":"Vet Parasitol"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1097\/01.qco.0000244055.46382.23","article-title":"Microsporidiosis: current status","volume":"19","author":"Didier ES","year":"2006","journal-title":"Curr Opin Infect Dis"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1097\/QCO.0b013e32834aa152","article-title":"Microsporidiosis: not just in AIDS patients","volume":"24","author":"Didier ES","year":"2011","journal-title":"Curr Opin Infect Dis"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/AEM.64.9.3332-3335.1998","article-title":"Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water","volume":"64","author":"Dowd SE","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"ref18","series-title":"Microsporidia: pathogens of opportunity","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/9781118395264.ch3","volume-title":"Epidemiology of microsporidia in human infections","author":"Fayer R","year":"2014"},{"issue":"14","key":"ref19","doi-asserted-by":"crossref","first-page":"4822","DOI":"10.1128\/AEM.02803-10","article-title":"Development of a multilocus sequence typing tool for high-resolution genotyping of Enterocytozoon bieneusi","volume":"77","author":"Feng Y","year":"2011","journal-title":"Appl Environ Microbiol"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.cll.2015.02.011","article-title":"Intestinal microsporidiosis","volume":"35","author":"Field AS","year":"2015","journal-title":"Clin Lab Med"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.rvsc.2016.06.006","article-title":"Zoonotic Enterocytozoon bieneusi genotypes found in Brazilian sheep","volume":"107","author":"Fiuza VRS","year":"2016","journal-title":"Res Vet Sci"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.vetpar.2015.12.008","article-title":"New findings of Enterocytozoon bieneusi in beef and dairy cattle in Brazil","volume":"216","author":"Fiuza VRS","year":"2016","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.parint.2015.01.002","article-title":"First report of Enterocytozoon bieneusi in pigs in Brazil","volume":"64","author":"Fiuza VRS","year":"2015","journal-title":"Parasitol Int"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1574-695X.2002.tb00592.x","article-title":"Detection of microsporidia, cryptosporidia and Giardia in swimming pools: a one-year prospective study","volume":"33","author":"Fournier S","year":"2002","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1590\/S0036-46652001000200011","article-title":"Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia River, Campinas, Brazil","volume":"43","author":"Franco RMB","year":"2001","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1128\/AEM.02737-12","article-title":"Molecular characterization of human-pathogenic microsporidia and Cyclospora cayetanensis isolated from various water sources in Spain: a year-long longitudinal study","volume":"79","author":"Galv\u00e1n AL","year":"2013","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1128\/JCM.01833-10","article-title":"First cases of microsporidiosis in transplant recipients in Spain and review of the literature","volume":"49","author":"Galv\u00e1n AL","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"7","key":"ref28","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1081\/ESE-120021124","article-title":"Removal of Encephalitozoon intestinalis, calicivirus, and coliphages by conventional drinking water treatment","volume":"38","author":"Gerba CP","year":"2003","journal-title":"J Environ Sci Health A Tox Hazard Subst Environ Eng"},{"issue":"6","key":"ref29","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1128\/AEM.02412-06","article-title":"Human enteropathogens load in activated sewage sludge and corresponding sewage sludge-end products","volume":"73","author":"Graczyk TK","year":"2007","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.trstmh.2007.02.018","article-title":"Quality of reclaimed waters: a public health need for source tracking of wastewater-derived protozoan enteropathogens in engineered wetlands","volume":"101","author":"Graczyk TK","year":"2007","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"5","key":"ref31","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1007\/s00436-010-1769-2","article-title":"Relationships among bather density, levels of human waterborne pathogens, and fecal coliform counts in marine recreational beach water","volume":"106","author":"Graczyk TK","year":"2010","journal-title":"Parasitol Res"},{"key":"ref32","article-title":"Waterborne pathogens: review for the drinking-water industry","author":"Guillot E","year":"2009","journal-title":"IWA Publishing"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1128\/AEM.02997-13","article-title":"Specificity and source of Enterocytozoon bieneusi genotypes in a drinking source watershed","volume":"80","author":"Guo Y","year":"2014","journal-title":"Appl Environ Microbiol"},{"issue":"10","key":"ref34","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1128\/AEM.03026-09","article-title":"Phylogenetic approach to the variability of the microsporidian, Enterocytozoon bieneusi and its implications for inter- and intrahost transmission","volume":"76","author":"Henriques-Gil N","year":"2010","journal-title":"Appl Environ Microbiol"},{"issue":"24","key":"ref35","doi-asserted-by":"crossref","first-page":"14219","DOI":"10.1021\/es504464t","article-title":"Occurrence, source, and human infection potential of Cryptosporidium and Enterocytozoon bieneusi in drinking source water in Shanghai, China, during a pig carcass disposal incident","volume":"48","author":"Hu Y","year":"2014","journal-title":"Environ Sci Technol"},{"issue":"7","key":"ref36","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"Mega 7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar S","year":"2016","journal-title":"Mol Biol Evol"},{"issue":"12","key":"ref37","doi-asserted-by":"crossref","first-page":"4490","DOI":"10.1128\/AEM.07708-11","article-title":"Identification of Encephalitozoon and Enterocytozoon (microsporidia) spores in stool and urine samples obtained from free-living South American Coatis (Nasua nasua)","volume":"78","author":"Lallo MA","year":"2012","journal-title":"Appl Environ Microbiol"},{"issue":"3-4","key":"ref38","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.vetpar.2012.06.030","article-title":"Encephalitozoon and Enterocytozoon (microsporidia) spores in stool from pigeons and exotic birds. Microsporidia spores in birds","volume":"190","author":"Lallo MA","year":"2012","journal-title":"Vet Parasitol"},{"issue":"6","key":"ref39","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1111\/jeu.12269","article-title":"Molecular characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in captive wildlife at Zhengzhou Zoo, China","volume":"62","author":"Li J","year":"2015","journal-title":"J Eukaryot Microbiol"},{"issue":"9","key":"ref40","article-title":"Molecular surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater","volume":"6","author":"Li N","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1645\/0022-3395(2003)089[0185:IOMSSI]2.0.CO;2","article-title":"Infectivity of microsporidia spores stored in water at environmental temperatures","volume":"89","author":"Li X","year":"2003","journal-title":"J Parasitol"},{"issue":"3","key":"ref42","doi-asserted-by":"crossref","first-page":"411","DOI":"10.2166\/wh.2016.192","article-title":"Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents","volume":"14","author":"Ma J","year":"2016","journal-title":"J Water Health"},{"key":"ref43","series-title":"Portaria 2914\/MS","year":"2011"},{"issue":"4","key":"ref44","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N","year":"1987","journal-title":"Mol Biol Evol"},{"issue":"3","key":"ref45","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.rvsc.2010.07.014","article-title":"Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animals","volume":"90","author":"Sant\u00edn M","year":"2011","journal-title":"Res Vet Sci"},{"issue":"1","key":"ref46","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1645\/GE-2184.1","article-title":"A zoonotic genotype of Enterocytozoon bieneusi in horses","volume":"96","author":"Sant\u00edn M","year":"2010","journal-title":"J Parasitol"},{"issue":"1","key":"ref47","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s11270-009-0086-9","article-title":"Occurrence of emerging contaminants in Brazilian drinking waters: a sewage-to-tap issue","volume":"206","author":"Sodr\u00e9 FF","year":"2010","journal-title":"Water Air Soil Poll"},{"issue":"6","key":"ref48","article-title":"Detection of microsporidia and identification of Enterocytozoon bieneusi in surface water by filtration followed by specific PCR","volume":"44","author":"Sparfel JM","year":"1997","journal-title":"J Eukaryot Microbiol"},{"issue":"4","key":"ref49","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1111\/j.1550-7408.2009.00393.x","article-title":"Characterization of genotypes of Enterocytozoon bieneusi in immunosuppressed and immunocompetent patient groups","volume":"56","author":"ten Hove RJ","year":"2009","journal-title":"J Eukaryot Microbiol"},{"key":"ref50","doi-asserted-by":"crossref","DOI":"10.1016\/B978-0-12-407706-5.00004-6","article-title":"Microsporidia and \u201cthe art of living together\u201d","volume":"82","author":"V\u00e1vra J","year":"2013","journal-title":"Adv Parasitol"},{"key":"ref51","series-title":"Microsporidia: pathogens of opportunity","author":"Weiss LM","year":"2014","edition":"1st"},{"key":"ref52","series-title":"Microsporidia: pathogens of opportunity","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/9781118395264.ch15","volume-title":"Clinical syndromes associated with microsporidiosis","author":"Weiss LM","year":"2014","edition":"1st"},{"issue":"2","key":"ref53","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1093\/infdis\/170.2.494","article-title":"Cryptosporidial and microsporidial infections in human immunodeficiency virus-infected patients in northeastern Brazil","volume":"170","author":"Wuhib T","year":"1994","journal-title":"J Infect Dis"},{"key":"ref54","first-page":"603","article-title":"Epidemiology, clinical, immune, and molecular profiles of microsporidiosis and cryptosporidiosis among HIV\/AIDS patients","volume":"5","author":"Wumba R","year":"2012","journal-title":"Int J Gen Med"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/mioc\/v112n6\/0074-0276-mioc-112-6-0404.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T22:32:38Z","timestamp":1692829958000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762017000600403&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["S0074-02762017000600403"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760160435","relation":{},"ISSN":["0074-0276"],"issn-type":[{"value":"0074-0276","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6]]}}}