{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:42:05Z","timestamp":1783953725856,"version":"3.55.0"},"reference-count":49,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2018\/23021-7"],"award-info":[{"award-number":["2018\/23021-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p> BACKGROUND  Toxocariasis is a neglected global zoonosis. The immunological diagnosis has setbacks that hinder further knowledge about its pathology, epidemiology, and public control measures, and lack of financial support and attention prevents innovative research. Although studies on synthetic peptides are common for several infectious pathologies, none evaluated chemically synthetic peptides for toxocariasis diagnosis.  OBJECTIVE  This study aimed to identify potential synthetic peptides from C-type lectin 1 (Tc-CTL-1) from Toxocara canis.  METHODS In silico analyses were made by five B-cell peptide prediction programs, 3-D modelling, BLASTp homology analysis, and signal-peptide identification. SPOT-synthesis was used for epitope mapping and assessed by dot-blot. Sera from non-infected and T. canis, Strongyloides venezuelensis, Ascaris suum, or Schistosoma mansoni-infected animals were used to assess the peptide\u2019s immunogenicity and cross-reactivity. The selection of potential immunogenic epitopes included the most immunogenic peptides with the least cross-reactivity.  FINDINGS  Fifty-five peptides were selected by in silico analysis. Dot-blot showed intense recognition by anti-Toxocara IgG and cross-reactivity with A. suum-infected mice. Selection criteria identified four epitopes with diagnostic potential.  MAIN CONCLUSIONS  The findings demonstrate that synthetic peptides should be explored for innovation of toxocariasis diagnosis, and suggest the adaptation of dot-blot using the SPOT-synthesis technique as a potential immunodiagnostic platform.<\/jats:p>","DOI":"10.1590\/0074-02760240111","type":"journal-article","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:44:32Z","timestamp":1741999472000},"source":"Crossref","is-referenced-by-count":4,"title":["Immunogenic mapping of potential epitopes from Tc-CTL-1 for the diagnosis of murine toxocariasis"],"prefix":"10.1590","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6047-3668","authenticated-orcid":false,"given":"Gabriela Rodrigues e","family":"Fonseca","sequence":"first","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo,  Brasil; Faculdade de Ci\u00eancias M\u00e9dicas da Santa Casa de S\u00e3o Paulo,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Laura","family":"Grossi de Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramayana Morais de Medeiros","family":"Brito","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Andreetta","family":"Corral","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard John","family":"Ward","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e2mela Aparecida","family":"de Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Bryan da Rocha","family":"Rihs","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Eduardo","family":"Cardozo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Keiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Toshio","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Vieira dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias M\u00e9dicas da Santa Casa de S\u00e3o Paulo,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo Cesar Borges","family":"Gryschek","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana Angelica Zevallos","family":"Lescano","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.ijpara.2013.07.004","article-title":"The epidemiology and public health importance of toxocariasis a zoonosis of global 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