{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:45:09Z","timestamp":1774982709753,"version":"3.50.1"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USAID\/PAHO","award":["7200AA22IO00001"],"award-info":[{"award-number":["7200AA22IO00001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p> BACKGROUND  In malaria-endemic regions, rapid diagnostic tests (RDTs) play a crucial role in promptly identifying infections, especially in remote areas with limited microscopy services.  OBJECTIVES  Conduct a cross-sectional, multi-site study to determine whether the local prevalence of mutations in the Plasmodium falciparum hrp2\/3 genes in false-negative RDTs has reached a threshold that might require a local or national change in diagnostic strategy in accordance with the WHO guidelines (2018).  METHODS  Individuals were screened for P. falciparum with microscopy and HRP2-based RDT at health facilities. Discordant results between these two tests triggered diagnostic confirmation by polymerase chain reaction (PCR) and detection of the pfhrp2\/pfhrp3 genes.  FINDINGS  Among the 347 patients included, false negatives constituted 4.61% (16\/347). Molecular analysis revealed all 16 false negatives were P. falciparum positive with hrp2 gene present, displaying high polymorphism. However, hrp3 gene deletion was observed in 93.8% (15\/16) of these cases.  MAIN CONCLUSIONS  The prevalence of false-negative RDTs is low, and these results were not linked to deletions in the hrp2 gene. This suggests that there is no immediate need to modify the RDTs used along the Colombian Pacific Coast. However, molecular surveillance for hrp2 deletions remains crucial to detect any potential increase in prevalence.<\/jats:p>","DOI":"10.1590\/0074-02760240134","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T20:37:30Z","timestamp":1749501450000},"source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of pfhrp2\/pfhrp3 gene deletions among patients with Plasmodium falciparum malaria with false-negative in the HRP2-based rapid diagnostic test in Colombia"],"prefix":"10.1590","volume":"120","author":[{"given":"Mario Javier","family":"Olivera","sequence":"first","affiliation":[{"name":"Instituto Nacional de Salud,  Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6133-8502","authenticated-orcid":false,"given":"Angela Patricia","family":"Guerra","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud,  Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana Jazm\u00edn","family":"Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud,  Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aravy Geohanna","family":"Su\u00e1rez-Jurado","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud,  Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda de la Paz","family":"Ade","sequence":"additional","affiliation":[{"name":"Pan-American Health Organization,  United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iv\u00e1n Mauricio","family":"C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Ministerio de Salud y Protecci\u00f3n Social,  Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"100246","DOI":"10.1016\/j.health.2023.100246","article-title":"A propensity score matching analysis using statistical methods for estimating the impact of intervention the cost of malaria and its impact on the health system","volume":"4","author":"Olivera MJ","year":"2023","journal-title":"Healthc Anal"},{"key":"ref2","year":"2024"},{"key":"ref3","year":"2023"},{"key":"ref4","first-page":"309","article-title":"Epidemiological evolution and historical anti-malarial control initiatives in Colombia, 1848-2019","volume":"30","author":"Padilla-Rodr\u00edguez JC","year":"2022","journal-title":"Infez Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1083\/jcb.103.4.1269","article-title":"Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodium 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