{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:26:54Z","timestamp":1787063214236,"version":"build-2736575974"},"reference-count":11,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["PROEP 2023-2025"],"award-info":[{"award-number":["PROEP 2023-2025"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["478232\/2013-6"],"award-info":[{"award-number":["478232\/2013-6"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["436097\/2018-4"],"award-info":[{"award-number":["436097\/2018-4"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ILMD-FIOCRUZ AMAZ\u00d4NIA","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"ILMD-FIOCRUZ AMAZ\u00d4NIA","award":["PROEP 2023-2025"],"award-info":[{"award-number":["PROEP 2023-2025"]}]},{"name":"ILMD-FIOCRUZ AMAZ\u00d4NIA","award":["478232\/2013-6"],"award-info":[{"award-number":["478232\/2013-6"]}]},{"name":"ILMD-FIOCRUZ AMAZ\u00d4NIA","award":["436097\/2018-4"],"award-info":[{"award-number":["436097\/2018-4"]}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["PROEP 2023-2025"],"award-info":[{"award-number":["PROEP 2023-2025"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["478232\/2013-6"],"award-info":[{"award-number":["478232\/2013-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["436097\/2018-4"],"award-info":[{"award-number":["436097\/2018-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND  Coinfections of Plasmodium parasites and the dengue virus have been linked to severe disease in some patients. The interactions between these two pathogens, particularly their effects on disease progression, highlight the clinical importance of understanding the mechanisms underlying the potential synergistic effects.  OBJECTIVES  The primary objective of this study was to investigate the adhesion dynamics of Plasmodium vivax-infected erythrocytes (Pv-iRBCs) in the presence of dengue virus (DENV) infection. By examining the interaction between these pathogens, the study aimed to provide insights into how coinfections might influence disease severity and progression.  METHODS  HepG2 cells were infected with DENV to observe changes in adhesion receptors and Pv-iRBCs adhesion capacity. Experiments using trypsin-treated Pv-iRBCs and UV-inactivated DENV dissected the adhesion process. Small molecule inhibitors were used to assess innate activation. ICAM-1 expression and its functional significance was quantified using a monoclonal anti-ICAM-1 antibody.  FINDINGS  We noted a significant increase in cytoadherence of Pv-iRBCs following DENV infection compared to mock conditions. Both trypsin treatment of Pv-iRBCs and UV inactivation of DENV led to a reduction in cytoadherence, underscoring their impact on the adhesion process. Notably, DENV infection induces an innate immune activation upregulating ICAM-1 on the cell surface and blocking with a monoclonal anti-ICAM-1 antibody significantly reduced the cytoadherence of Pv-iRBCs.  MAIN CONCLUSIONS  Elevated ICAM-1 levels on DENV-permissive cells may not only trap parasites within several niches but also contribute to endothelial and haematological disturbances in individuals with coinfections. Further research is required to fully elucidate the roles of cytoadherence and immune activation in the pathogenesis of dengue and malaria coinfections.<\/jats:p>","DOI":"10.1590\/0074-02760240185","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T22:17:09Z","timestamp":1745965029000},"source":"Crossref","is-referenced-by-count":1,"title":["Impact of dengue virus infection on the cytoadherence of Plasmodium vivax-infected erythrocytes"],"prefix":"10.1590","volume":"120","author":[{"given":"Maria Geuziane Soares","family":"da Cruz","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Universidade Federal do Amazonas,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaella Oliveira","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Universidade Federal do Amazonas,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gloria Teixeira","family":"Sousa","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio TM","family":"Costa","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vin\u00edcius Guimar\u00e3es","family":"de Lacerda","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o de Medicina Tropical Dr Heitor Vieira Dourado,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie Costa Pinto","family":"Lopes","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2996-0446","authenticated-orcid":false,"given":"Pritesh","family":"Lalwani","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Universidade Federal do Amazonas,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.ijid.2023.01.033","article-title":"Antibody cross-reactivity and evidence of susceptibility to emerging Flaviviruses in the dengue-endemic Brazilian Amazon","volume":"129","author":"Salgado BB","year":"2023","journal-title":"Int J Infect Dis"},{"issue":"10","key":"ref2","article-title":"P vivax malaria and dengue fever co-infection: a cross-sectional study in the Brazilian Amazon","volume":"8","author":"Siqueira AM","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"ref3","article-title":"Total parasite biomass but not peripheral parasitaemia is associated with endothelial and haematological perturbations in","volume":"10","author":"Dos-Santos JCK","year":"2021","journal-title":"Plasmodium vivax patients. eLife"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1086\/654815","article-title":"On the cytoadhesion of Plasmodium vivax-infected erythrocytes","volume":"202","author":"Carvalho BO","year":"2010","journal-title":"J Infect Dis"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"e67","DOI":"10.1093\/cid\/cis615","article-title":"Postmortem characterization of patients with clinical diagnosis of Plasmodium vivax malaria to what extent does this parasite kill?","volume":"55","author":"Alexandre Maa","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1016\/j.ajpath.2011.01.016","article-title":"The neuropathology of fatal cerebral malaria in malawian children","volume":"178","author":"Dorovini-Zis K","year":"2011","journal-title":"Am J Pathol"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.molbiopara.2009.09.007","article-title":"Continued cytoadherence of Plasmodium falciparum infected red blood cells after antimalarial treatment","volume":"169","author":"Hughes KR","year":"2010","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.cell.2016.07.055","article-title":"Malaria biology and disease","volume":"167","author":"Cowman AF","year":"2016","journal-title":"Cell"},{"issue":"4","key":"ref9","article-title":"Rosetting in Plasmodium vivax a cytoadhesion phenotype associated with anaemia","volume":"7","author":"Hirayama K","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.virol.2012.09.038","article-title":"Essential role of RIG-I in the activation of endothelial cells by dengue virus","volume":"435","author":"Martins NB","year":"2013","journal-title":"Virology"},{"issue":"1","key":"ref11","article-title":"Dengue virus enhances thrombomodulin and ICAM-1 expression through the macrophage migration inhibitory factor induction of the MAPK and PI3K signaling pathways","volume":"8","author":"Yeh T-M","year":"2013","journal-title":"PLoS One"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0074-02762025000101120&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T22:17:26Z","timestamp":1745965046000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762025000101120&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":11,"alternative-id":["S0074-02762025000101120"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760240185","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e240185"}}