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Inst. Oswaldo Cruz"],"abstract":"<jats:p> BACKGROUND  The treatment of the early chronic phase of Chagas disease (CD) may result in high rates of parasitological cure, which may be associated with clinical benefits.  OBJECTIVES  To evaluate children with CD from the Jequitinhonha Valley, MG, Brazil, treated with benznidazole (BZ), employing classic and alternative methodologies.  METHODS  Before and after treatment, nine individuals were examined by haemoculture, polymerase chain reaction (PCR), conventional enzyme-linked immunosorbent assay (ELISA), electrocardiogram, echocardiogram, and thoracic and gastrointestinal X-ray. Eight individuals were in the indeterminate clinical form of CD, and one was in the mild cardiac form. After treatment, all individuals were re-evaluated periodically for 4-26 years using the same methodologies cited and anti-live trypomastigotes antibodies by flow-cytometry-FC-ALTA and quantitative PCR (qPCR).  FINDINGS  The cure rate by the classic cure criteria was 33.33%. By the alternative cure criteria using FC-ALTA and qPCR, the rates of cure were 50% and 78%, respectively. Post-treatment clinical evaluations revealed stability in 5\/9 and discrete clinical evolution in 4\/9 individuals.  MAIN CONCLUSIONS  It was demonstrated the effectiveness of BZ treatment in recent chronic infections of CD with low or higher rates of parasitological cure according to the cure criterion used after long-term follow-up. The clinical status of the individuals remained stable or evolved slowly, suggesting clinical benefits from BZ treatment.<\/jats:p>","DOI":"10.1590\/0074-02760240229","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T10:57:58Z","timestamp":1747393078000},"source":"Crossref","is-referenced-by-count":3,"title":["Parasitological cure and clinical benefits of benznidazole treatment in patients from the Jequitinhonha Valley, MG, Brazil, with recent chronic infection by Trypanosoma cruzi II"],"prefix":"10.1590","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1565-4707","authenticated-orcid":false,"given":"Marta","family":"de Lana","sequence":"first","affiliation":[{"name":"Universidade Federal de Ouro Preto,  Brazil; Universidade Federal de Ouro Preto,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourena","family":"Tomazelli","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ouro Preto,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falio","family":"Santoro","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ouro Preto,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girley Francisco","family":"Machado de Assis","sequence":"additional","affiliation":[{"name":"Universidade Federal de Juiz de Fora,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Marques","family":"Milagre","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ouro Preto,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glaucia Diniz","family":"Alessio","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato Afonso","family":"Salgado","sequence":"additional","affiliation":[{"name":"Hospital Nossa Senhora das Gra\u00e7as,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olindo Assis","family":"Martins-Filho","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Albajar-Vi\u00f1as","sequence":"additional","affiliation":[{"name":"World Health Organization,  Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ros\u00e1lia Morais","family":"Torres","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","series-title":"American trypanosomiasis Chagas disease one hundred years of research","first-page":"89","volume-title":"Geographical distribution of Chagas disease","author":"Guhl F","year":"2017","edition":"2"},{"key":"ref2","year":"2023"},{"issue":"1","key":"ref3","first-page":"e00023","article-title":"Chagas disease in the United States a public health approach","volume":"33","author":"Bern C","year":"2020","journal-title":"Clin Microbiol Rev"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1111\/tmi.13915","article-title":"Oral transmission of Chagas disease from a one health approach a systematic review","volume":"28","author":"L\u00f3pez-Garc\u00eda A","year":"2023","journal-title":"Trop Med Int Health"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"06","DOI":"10.1016\/j.ejim.2017.05.001","article-title":"Chagas disease in Europe a review for the internist in the globalized world","volume":"43","author":"Spinello A","year":"2017","journal-title":"Eur J Inter Med"},{"issue":"9723","key":"ref6","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi-Jr A","year":"2010","journal-title":"Lancet"},{"issue":"9","key":"ref7","first-page":"767","article-title":"Chagas disease an overview of clinical and epidemiological aspects","volume":"62","author":"Nunes MCP","year":"2013","journal-title":"J Am Coll Cardiol"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ahj.2008.04.001","article-title":"Rationale and design of a randomized placebo-controlled trial assessing the effects of etiologic treatment in Chagas' cardiomyopathy the benznidazole evaluation for interrupting trypanosomiasis (BENEFIT)","volume":"156","author":"Marin-Neto JA","year":"2008","journal-title":"Am Heart J"},{"issue":"12","key":"ref9","first-page":"e169","article-title":"Chagas cardiomyopathy an update of current clinical knowledge and management: a scientific statement from the American Heart Association","volume":"138","author":"Nunes MCP","year":"2018","journal-title":"Circulation"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1590\/S0074-02762009000900023","article-title":"Therapy, diagnosis and prognosis of chronic Chagas disease: insight gained in Argentina","author":"Sosa-Estani S","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1590\/S0074-02762002000100001","article-title":"A critical review on chagas disease chemotherapy","volume":"97","author":"Coura JR","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1097\/01.qco.0000247592.21295.a5","article-title":"Etiological treatment in patients infected by Trypanosoma cruzi experiences in Argentina","volume":"19","author":"Sosa-Estani S","year":"2006","journal-title":"Curr Opin Infect Dis"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1590\/S0074-02762011000600001","article-title":"Chronic phase of Chagas disease why should it be treated? A comprehensive review","volume":"106","author":"Coura JR","year":"2011","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"7","key":"ref14","article-title":"Feasibility, drug safety, and effectiveness of etiological treatment programs for Chagas disease in Honduras, Guatemala, and Bolivia 10-year experience of M\u00e9decins Sans Fronti\u00e8res","volume":"3","author":"Yun O","year":"2009","journal-title":"Plos Negl Tropi Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2174\/187152106776359020","article-title":"Advances in Chagas disease chemotherapy","volume":"5","author":"Guedes PM","year":"2006","journal-title":"Antiinfect Agents Med Chem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/S0140-6736(96)04128-1","article-title":"Randomised trial of efficacy of benznidazole in treatment of early Trypanosoma cruzi infection","volume":"348","author":"Andrade ALSS","year":"1996","journal-title":"Lancet"},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"526","DOI":"10.4269\/ajtmh.1998.59.526","article-title":"Efficacy of chemotherapy with benznidazole in children in the indeterminate phase of Chagas' disease","volume":"59","author":"Sosa-Estani S","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"106050","DOI":"10.1016\/j.actatropica.2021.106050","article-title":"Trypanocidal therapy among children infected by Trypanosoma cruzi Serological and electrocardiographic changes over a mean twenty-five-years follow-up period","volume":"222","author":"Suasn\u00e1bar S","year":"2021","journal-title":"Acta Trop"},{"issue":"5","key":"ref19","first-page":"365","article-title":"Longitudinal study and specific chemotherapy in children with chronic Chagas disease residing in a low endemicity area of Argentina","volume":"37","author":"Streiger ML","year":"2004","journal-title":"Rev Soc Bras Med Trop"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.actatropica.2013.07.003","article-title":"Benznidazole treatment in chronic children infected with Trypanosoma cruzi Serological and molecular follow-up of patients and identification of Discrete Typing Units","volume":"128","author":"Rumi MMM","year":"2013","journal-title":"Acta Trop"},{"issue":"2","key":"ref21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003465","article-title":"Follow-up of an asymptomatic Chagas disease population of children after treatment with nifurtimox (Lampit) in a sylvatic endemic transmission area of Colombia","volume":"9","author":"Bianchi F","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"Spe","key":"ref22","first-page":"07","article-title":"II Consenso Brasileiro em Doen\u00e7a de Chagas 2015","volume":"25","author":"Dias JCP","year":"2016","journal-title":"Epidemiol Serv Sa\u00fade"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/cdli.2.5.569-573.1995","article-title":"Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas disease","volume":"2","author":"Martins-Filho OA","year":"1995","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/CDLI.8.1.112-118.2001","article-title":"Anti-Trypanosoma cruzi immunoglobulin G1 can be a useful tool for diagnosis and prognosis of human Chagas disease","volume":"8","author":"Cordeiro FD","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1590\/S0036-46652006000200005","article-title":"Seroprevalence of Chagas disease in schoolchildren from two municipalities of Jequitinhonha Valley, Minas Gerais, Brazil; six years following the onset of epidemiological surveillance","volume":"48","author":"Borges JD","year":"2006","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1590\/S0037-86822012000100008","article-title":"In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity","volume":"45","author":"Santos LS","year":"2012","journal-title":"Rev Soc Bras Med Trop"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1590\/0037-8682-0077-2013","article-title":"Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil","volume":"46","author":"Silva JCVO","year":"2013","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref28","article-title":"Trypanosoma cruzi discret typing units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies","author":"Oliveira MT","year":"2015","journal-title":"Parasit Vectors"},{"key":"ref29","article-title":"Terap\u00eautica espec\u00edfica. In: Dias JCP, Coura JR, editors","author":"Can\u00e7ado JR","year":"1997","journal-title":"Fiocruz"},{"key":"ref30","article-title":"Consenso Brasileiro em Doen\u00e7a de Chagas, 2005","author":"Rocha A","year":"2005","journal-title":"Rev Soc Bras Med Trop"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1590\/S0037-86821989000100004","article-title":"Hemocultures for the parasitological diagnosis of human chronic Chagas' disease","volume":"22","author":"Chiari E","year":"1989","journal-title":"Rev Soc Bras Med Trop"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4269\/ajtmh.1999.60.205","article-title":"Chagas' disease diagnosis comparative analysis of parasitologic, molecular, and serologic methods","volume":"60","author":"Gomes ML","year":"1999","journal-title":"Am J Trop Med Hyg"},{"issue":"8","key":"ref33","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1128\/CVI.00274-12","article-title":"Posttherapeutic cure criteria in Chagas' disease conventional serology followed by supplementary serological parasitological, and molecular tests","volume":"19","author":"Machado-de-Assis GF","year":"2012","journal-title":"Clin Vaccine Immunol"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.actatropica.2012.05.002","article-title":"Real-time PCR strategy for parasite quantification in blood and tissue samples of experimental Trypanosoma cruzi infection","volume":"123","author":"Caldas S","year":"2012","journal-title":"Acta Trop"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0166-6851(03)00093-8","article-title":"Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR","volume":"129","author":"Cummings KL","year":"2003","journal-title":"Mol Biochem Parasitol"},{"key":"ref36","first-page":"55","article-title":"Enzyme immunoassays in diagnostic medicine Theory and practice","volume":"53","author":"Voller A","year":"1976","journal-title":"Bull World Health Organ"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/0035-9203(82)90184-5","article-title":"Effect of specific chemotherapy on the levels of lytic antibodies in Chagas's disease","volume":"76","author":"Krettli AU","year":"1982","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref38","doi-asserted-by":"crossref","DOI":"10.5935\/abc.20160054","article-title":"III Diretrizes da Sociedade Brasileira de Cardiologia sobre an\u00e1lise e emiss\u00e3o de laudos eletrocardiogr\u00e1ficos","author":"Pastore CA","year":"2016","journal-title":"Arq Bras Cardiol"},{"issue":"1","key":"ref39","first-page":"40","article-title":"Value of the cardiothoracic index on the evaluation of myocardial involvement Correlation with echocardiographic evaluation","volume":"38","author":"Pereira-Barretto AC","year":"1983","journal-title":"Rev Hosp Clin Fac Med S\u00e3o Paulo"},{"issue":"2","key":"ref40","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1590\/S0066-782X2003000200009","article-title":"Value of the radiological study of the thorax for diagnosing left ventricular dysfunction in Chagas' disease","volume":"80","author":"Perez AA","year":"2003","journal-title":"Arq Bras Cardiol"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.echo.2014.10.003","article-title":"Recommendations for cardiac chamber quantification by echocardiography in adults an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging","volume":"28","author":"Lang RM","year":"2015","journal-title":"J Am Soc Echocardiogr"},{"issue":"2","key":"ref42","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.meegid.2011.12.009","article-title":"The revised Trypanosoma cruzi subspecific nomenclature rationale, epidemiological relevance and research applications","volume":"12","author":"Zingales B","year":"2012","journal-title":"Infect Genet Evol"},{"key":"ref43","article-title":"Revisiting the posttherapeutic cure criterion in Chagas disease: time for new methods, more questions, doubts, and polemics or time to change old concepts?","author":"Lana M","year":"2015","journal-title":"BioMed Res Int"},{"key":"ref44","article-title":"Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations","author":"Britto CC","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"ref45","first-page":"1236","article-title":"Validation of the NAT Chagas IVD Kit for the detection and quantification of Trypanosoma cruzi in blood samples of patients with Chagas disease","volume":"13","author":"Moreira OC","year":"2023","journal-title":"Life (Basel)"},{"key":"ref46","article-title":"Diagnosis of Chagas disease: what has been achieved? What remains to be done with regard to diagnosis and follow up studies?","author":"Gomes YM","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"8","key":"ref47","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1590\/S0074-02762009000800011","article-title":"Clinical and laboratory status of patients with chronic Chagas disease living in a vector-controlled area in Minas Gerais, Brazil, before and nine years after aetiological treatment","volume":"104","author":"Lana M","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"7","key":"ref48","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1590\/0074-0276130122","article-title":"A serological, parasitological and clinical evaluation of untreated Chagas disease patients and those treated with benznidazole before and thirteen years after intervention","volume":"108","author":"Machado-de-Assis GF","year":"2013","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ijid.2018.05.019","article-title":"Course of serological tests in treated subjects with chronic Trypanosoma cruzi infection a systematic review and meta-analysis of individual individual data","volume":"73","author":"Sguassero Y","year":"2018","journal-title":"Int J Infect Dis"},{"issue":"5","key":"ref50","first-page":"1107","article-title":"Double-blind study to evaluate flow cytometry analysis of anti-live trypomastigote antibodies for monitoring treatment efficacy in cases of human Chagas' disease","volume":"9","author":"Martins-Filho OA","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"issue":"2","key":"ref51","first-page":"191","article-title":"Specific treatment evaluation for Trypanosoma cruzi in children, in the evolution of the indeterminate phase","volume":"33","author":"Silveira CAN","year":"2000","journal-title":"Rev Soc Bras Med Trop"},{"issue":"10","key":"ref52","doi-asserted-by":"crossref","first-page":"733","DOI":"10.7326\/0003-4819-144-10-200605160-00006","article-title":"Long-term cardiac outcomes of treating chronic Chagas disease with benznidazole versus no treatment A nonrandomized trial","volume":"144","author":"Viotti R","year":"2006","journal-title":"Ann Inter Med"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"103e113","DOI":"10.1016\/j.micinf.2007.10.009","article-title":"Etiological treatment during early chronic indeterminate Chagas disease incites an activated status on innate and adaptive immunity associated with a type 1-modulated cytokine pattern","volume":"10","author":"Sathler-Avelar R","year":"2008","journal-title":"Microbes Infect"},{"issue":"1","key":"ref54","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1590\/S0036-46652002000100006","article-title":"Long term evaluation of etiological treatment of Chagas disease with benznidazole","volume":"44","author":"Can\u00e7ado JR","year":"2002","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"7","key":"ref55","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1590\/S0074-02762009000700008","article-title":"Treatment and seroconversion in a cohort of children suffering from recent chronic Chagas infection in Yoro, Honduras","volume":"104","author":"Escrib\u00e0 JM","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"ref56","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1590\/0037-8682-0415-2016","article-title":"High seroconversion rates in Trypanosoma cruzi chronic infection treated with benznidazole in people under 16 years in Guatemala","volume":"49","author":"Brum-Soares L","year":"2016","journal-title":"Rev Soc Bras Med Trop"},{"issue":"12","key":"ref57","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010968","article-title":"Long-term cardiology outcomes in children after early treatment for Chagas disease, an observational study","volume":"16","author":"Gonz\u00e1lez NL","year":"2022","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0074-02762025000101124&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T10:58:14Z","timestamp":1747393094000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762025000101124&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":57,"alternative-id":["S0074-02762025000101124"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760240229","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e240229"}}