{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:59:28Z","timestamp":1770447568778,"version":"3.49.0"},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND  The Haemagogus genus includes nine mosquito species reported in Brazil, each with distinct distribution patterns. Haemagogus leucocelaenus, a major yellow fever vector, is widely distributed throughout the country, while Haemagogus leucophoebus, a morphologically similar species, has only been identified in Acre State.  OBJECTIVES  This study evaluated the presence of Haemagogus species in southern Brazil by comparing their morphological and molecular characteristics.  METHODS  Mosquitoes were collected from five municipalities in southern Santa Catarina State, Brazil. Each specimen was identified morphologically and photographed. Genomic DNA was extracted, and a Cytochrome C Oxidase Subunit I (COI) gene fragment was amplified using polymerase chain reaction (PCR). The positive amplicons were sequenced for molecular identification.  FINDINGS  New records of Hg. leucocelaenus were found in Santa Rosa de Lima, Rio Fortuna, Bra\u00e7o do Norte, S\u00e3o Martinho, and Pedras Grandes, located at the southern edge of the Atlantic Forest. This study expands the known distribution of Hg. leucocelaenus, the only Haemagogus species identified in the area, with 91 specimens collected. Although some specimens exhibited morphological variations that might lead to misidentification as Hg. leucophoebus, molecular identification confirmed that all were Hg. leucocelaenus.  MAIN CONCLUSIONS  This study is the first to report Hg. leucocelaenus in Santa Catarina, Brazil, and provides DNA barcoding sequences from southern Brazil. This method offers a reliable alternative for species identification, especially when combined with morphological analysis. Further molecular studies are needed to determine whether the morphological variations observed indicate intraspecific differences.<\/jats:p>","DOI":"10.1590\/0074-02760240240","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T09:44:10Z","timestamp":1751967850000},"source":"Crossref","is-referenced-by-count":0,"title":["Expanded range of Haemagogus leucocelaenus in yellow fever hotspots: new findings from Santa Catarina State, southern Brazil"],"prefix":"10.1590","volume":"120","author":[{"given":"Sabrina Fernandes","family":"Cardoso","sequence":"first","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil; Secretaria da Sa\u00fade do Estado de Santa Catarina,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iara Carolini","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Akemi Oliveira","family":"Kikuti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre Akira Gonzaga","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 N\u00f3brega","family":"Pitaluga","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3400-3950","authenticated-orcid":false,"given":"Lu\u00edsa Damazio P","family":"Rona","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil; Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever","volume":"64","author":"Monath TP","year":"2015","journal-title":"J Clin Virol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s00705-023-05835-1","article-title":"Renaming of the genus Flavivirus to Orthoflavivirus and extension of binomial species names within the family Flaviviridae","volume":"168","author":"Postler TS","year":"2023","journal-title":"Arch Virol"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adg9204","article-title":"Genomic epidemiology unveils the dynamics and spatial corridor behind the yellow fever virus outbreak in Southern Brazil","volume":"9","author":"Giovanetti M","year":"2023","journal-title":"Sci Adv"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1080\/22221751.2019.1568180","article-title":"Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016-2018","volume":"8","author":"Abreu FVS","year":"2019","journal-title":"Emerg Microbes Infect"},{"key":"ref5","article-title":"Mosquito studies (Diptera, Culicidae)","author":"Arnell JH","year":"1973","journal-title":"Contributions of the American Entomological Institute"},{"key":"ref6","series-title":"Culicidologia m\u00e9dica: identifica\u00e7\u00e3o, biologia e epidemiologia. vol. 2","author":"Forattini OP","year":"2002"},{"key":"ref7","article-title":"Mosquitoes of the World. Baltimore","author":"Wilkerson RC","year":"2021","journal-title":"Johns Hopkins University Press"},{"key":"ref8","article-title":"Ecological relationships of Haemagogus spegazzinii (Diptera Culicidae) in a semiarid area of Brazil","volume":"53","author":"Silva-Inacio CL","year":"2020","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.11646\/zootaxa.1779.1.6","article-title":"New records of Haemagogus (Haemagogus) from northern and northeastern Brazil (Diptera Culicidae, Aedini)","volume":"1779","author":"Alencar J","year":"2008","journal-title":"Zootaxa"},{"key":"ref10","series-title":"Principais mosquitos de import\u00e2ncia sanit\u00e1ria no Brasil","author":"Consoli RAGB","year":"1994"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/aesa\/45.4.529","article-title":"The taxonomic status of the Aedes leucocelaenus complex with descriptions of two new forms (Diptera, Culicidae)","volume":"45","author":"Galindo P","year":"1952","journal-title":"Ann Entomol Soc Am"},{"issue":"3","key":"ref12","first-page":"221","article-title":"Revis\u00e3o de mosquitos Haemagogus Williston (Diptera Culicidae) do Brasil","volume":"21","author":"Marcondes CB","year":"2010","journal-title":"Rev Biomed"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"101","DOI":"10.5123\/S1679-49742010000200003","article-title":"Ecologia de Haemagogus e Sabethes (Diptera Culicidae) em \u00e1reas epizo\u00f3ticas do v\u00edrus da febre amarela, Rio Grande do Sul, Brasil","volume":"19","author":"Gomes AC","year":"2010","journal-title":"Epidemiol Serv Sa\u00fade"},{"key":"ref14","article-title":"Mapping environmental suitability of Haemagogus and Sabethes spp mosquitoes to understand sylvatic transmission risk of yellow fever virus in Brazil","volume":"16","author":"Li S","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"ref15","series-title":"Situa\u00e7\u00e3o da febre Amarela em Santa Catarina","year":"2021"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jmedent\/41.5.01","article-title":"DNA barcodes can distinguish species of Indian mosquitoes (Diptera Culicidae)","volume":"44","author":"Kumar NP","year":"2007","journal-title":"J Med Entomol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies","volume":"32","author":"Nguyen LT","year":"2015","journal-title":"Mol Biol Evol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive tree of life (iTOL) v5 an online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic I","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"ref19","first-page":"1","article-title":"Mosquito studies (Diptera Culicidae). XXVIII. The New World species formerly placed in Aedes (Finlaya)","volume":"8","author":"Zavortink TJ","year":"1972","journal-title":"Contr Am Ent Inst"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"16948","DOI":"10.1038\/s41598-020-73790-x","article-title":"Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera Culicidae)","volume":"10","author":"da Silva FS","year":"2020","journal-title":"Sci Rep"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"669379","DOI":"10.3389\/fcell.2021.669379","article-title":"The mitochondrial response to DNA damage","volume":"9","author":"Rong Z","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1051\/parasite\/2019053","article-title":"Altitudinal variations in wing morphology of Aedes albopictus (Diptera, Culicidae) in Albania, the region where it was first recorded in Europe","volume":"26","author":"Prudhomme J","year":"2019","journal-title":"Parasite"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/syen.12559","article-title":"A phylogenomic approach to species delimitation in the mango fruit fly (Bactrocera frauenfeldi) complex a new synonym of an important pest species with variable morphotypes (Diptera: Tephritidae)","volume":"48","author":"Doorenweerd C","year":"2022","journal-title":"Systematic Entomology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s13592-024-01057-9","article-title":"Morphological and molecular evidence for considering Xylocopa nigrocincta as the senior synonym of Xylocopa suspecta (Apidae Xylocopini)","volume":"55","author":"Agostini JC","year":"2024","journal-title":"Apidologie"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"107377","DOI":"10.1016\/j.actatropica.2024.107377","article-title":"COI DNA barcoding to differentiate Haemagogus janthinomys and Haemagogus capricornii (Diptera Culicidae) mosquitoes","volume":"259","author":"Telles-de-Deus J","year":"2024","journal-title":"Acta Trop"},{"key":"ref26","series-title":"Spatial data science: with applications in R","author":"Pebesma E","year":"2023","edition":"1"},{"key":"ref27","first-page":"439","article-title":"Simple features for R standardized support for spatial vector data","volume":"10","author":"Pebesma E","year":"2018","journal-title":"R Journal"},{"key":"ref28","article-title":"maps: draw geographical maps","author":"Becker RA","year":"2023","journal-title":"R package version 3.4.2."},{"key":"ref29","article-title":"mapdata: Extra map databases","author":"Becker RA","year":"2022","journal-title":"R package version 2.3.1."},{"key":"ref30","series-title":"R: a language and environment for statistical computing","year":"2014"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0074-02762025000101137&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T09:44:15Z","timestamp":1751967855000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762025000101137&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":30,"alternative-id":["S0074-02762025000101137"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760240240","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e240240"}}