{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T13:02:49Z","timestamp":1772197369556,"version":"3.50.1"},"reference-count":39,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cooperation and Immigration Directorate, Govern de les Illes Balears, National Institute for Health of Peru","award":["OCDS-CUD2022\/07"],"award-info":[{"award-number":["OCDS-CUD2022\/07"]}]},{"name":"Cooperation and Immigration Directorate, Govern de les Illes Balears, National Institute for Health of Peru","award":["R0 project"],"award-info":[{"award-number":["R0 project"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND While Chagas disease (CD) has been controlled in many South American regions, the Amazon basin has emerged as a new focus of transmission. Metropolitan Iquitos (Loreto, Peru), has recently shown signs of potential disease emergence.  OBJECTIVE To assess the risk of CD transmission by evaluating triatomine presence and infection rates in households across Iquitos and nearby communities.  METHODS Entomological surveillance was conducted in domestic and peridomestic environments, following blood donor screenings (2011-2018) that confirmed local Trypanosoma cruzi cases. Triatomines were collected manually, with traps, and through community reporting. Specimens were identified, epidemiological indices calculated, and infestation risk factors analysed using penalised logistic regression, receiver operating characteristic (ROC)\/area under the curve (AUC) metrics, and exploratory principal component analysis (PCA).  FINDINGS Of 142 houses visited, 113 were inspected, yielding a density index of 0.26. Nine houses were infested, mostly in Loboyacu, with 29 adult triatomines collected \u2014 by Rhodnius robustus (89.7%) and Panstrongylus geniculatus. T. cruzi infection was confirmed, and palm roofs emerged as the strongest predictor of infestation [odds ratio (OR) &gt; 16, p &lt; 0.001].  MAIN CONCLUSIONS This first evidence of T. cruzi circulation in sylvatic triatomines within Metropolitan Iquitos highlights an emerging risk of CD. Although vectors remain scarce, palm-roofed houses, deforestation, and urban expansion may facilitate future transmission.<\/jats:p>","DOI":"10.1590\/0074-02760240257","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T12:12:06Z","timestamp":1772194326000},"source":"Crossref","is-referenced-by-count":0,"title":["Detection of Trypanosoma cruzi-infected triatomines in Iquitos: possible incipient colonisation in the largest metropolis of the Peruvian Amazon"],"prefix":"10.1590","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8084-2723","authenticated-orcid":false,"given":"Fabiola","family":"D\u00edaz-Soria","sequence":"first","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Zevallos","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Amazonia Peruana,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan","family":"Cabrera-Campos","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Sinti-Hesse","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e0","family":"Jaume-Ramis","sequence":"additional","affiliation":[{"name":"University of the Balearic Islands,  Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wieslawa","family":"Alava-Flores","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darcy","family":"Acho-Bernuy","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Vega-Chirinos","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Pinto-Caballero","sequence":"additional","affiliation":[{"name":"National Institute of Health,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"Ramal-Asayag","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Amazonia Peruana,  Peru; Hospital Regional de Loreto,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mois\u00e9s","family":"Sihuincha","sequence":"additional","affiliation":[{"name":"Arzobispo Loayza National Hospital,  Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Paredes-Esquivel","sequence":"additional","affiliation":[{"name":"University of the Balearic Islands,  Spain; Biomedical Research Networking Center for Infectious Diseases,  Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","year":"2024"},{"issue":"10115","key":"ref2","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0140-6736(17)31612-4","article-title":"Chagas disease","volume":"391","author":"P\u00e9rez-Molina JA","year":"2018","journal-title":"Lancet"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1089\/vbz.2009.0015","article-title":"Entomological factors affecting the low endemicity of Chagas disease in Nazca, southwestern Peru","volume":"10","author":"Paredes-Esquivel C","year":"2010","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"556","DOI":"10.3389\/fvets.2020.00556","article-title":"Chagas disease in pregnant women in the Peruvian Amazon basin. Cross-sectional study","volume":"7","author":"Ramos-Rincon JM","year":"2020","journal-title":"Front Vet Sci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"S33","DOI":"10.1590\/S0102-311X2007001300005","article-title":"Chagas disease and globalization of the Amazon","volume":"23","author":"Briceho-Leon R","year":"2007","journal-title":"Cad Saude Publica"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1590\/S0074-02762012000200001","article-title":"Risks of endemicity, morbidity and perspectives regarding the control of Chagas disease in the Amazon Region","volume":"107","author":"Coura JR","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"ref7","first-page":"70","article-title":"Clinical and epidemiological characteristics of cases of acute Chagas disease in the Peruvian Amazon basin, 2009-2016","volume":"38","author":"Vega S","year":"2021","journal-title":"Rev Peru Med Exp Salud Publica"},{"issue":"Suppl. I","key":"ref8","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1590\/S0074-02762007005000098","article-title":"Chagas disease in the Amazon Region","volume":"102","author":"Aguilar HM","year":"2007","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005422","article-title":"Ten years of Chagas disease research: looking back to achievements, looking ahead to challenges","volume":"11","author":"Dumonteil E","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref10"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.actatropica.2015.10.020","article-title":"The impact of indoor residual spraying of deltamethrin on dengue vector populations in the Peruvian Amazon","volume":"154","author":"Paredes-Esquivel C","year":"2016","journal-title":"Acta Trop"},{"issue":"4","key":"ref12","first-page":"288","article-title":"First case of acute Chagas disease in the Peruvian central jungle: investigation of relatives, vectors, and reservoirs [in Spanish]","volume":"23","author":"Vega S","year":"2006","journal-title":"Rev Peru Med Exp Salud Publica"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1590\/0037-8682-1195-2013","article-title":"New focus of active transmission of Chagas' disease in indigenous populations in the Peruvian Amazon basin","volume":"46","author":"Cabrera R","year":"2013","journal-title":"Rev Soc Bras Med Trop"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"473","DOI":"10.4269\/ajtmh.2008.79.473","article-title":"Eight year old with fever, hepatomegaly, and positive thick smear","volume":"79","author":"Asayag CR","year":"2008","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1590\/S0036-46652010000500009","article-title":"Epidemiological investigation of an acute case of Chagas disease in an area of active transmission in Peruvian Amazon Region","volume":"52","author":"Cabrera R","year":"2010","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"ref16","year":"2005"},{"key":"ref17","year":"2005"},{"key":"ref18","year":"2023"},{"key":"ref19","series-title":"An R Companion to Applied Regression","author":"Fox J","year":"2019","edition":"3rd ed"},{"key":"ref20","author":"Heinze G"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/1471-2105-12-77","article-title":"pROC: an open-source package for R and S+ to analyze and compare ROC curves","volume":"12","author":"Robin X","year":"2011","journal-title":"BMC Bioinformatics"},{"key":"ref22","series-title":"ggplot2: elegant graphics for data analysis","author":"Wickham H","year":"2016"},{"issue":"7","key":"ref23","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1128\/jcm.27.7.1477-1482.1989","article-title":"Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction","volume":"27","author":"Moser DR","year":"1989","journal-title":"J Clin Microbiol"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0166-6851(89)90082-0","article-title":"Sensitive detection and schizodeme classification of Trypanosoma cruzi cells by amplification of kinetoplast minicircle DNA sequences: use in diagnosis of Chagas' disease","volume":"33","author":"Sturm NR","year":"1989","journal-title":"Mol Biochem Parasitol"},{"issue":"11","key":"ref25","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/S0020-7519(01)00238-7","article-title":"Characterisation of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages","volume":"31","author":"Brisse S","year":"2001","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1590\/S0074-02762002000200005","article-title":"The triatomines of northern 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Amazonia: a risk for human populations?","volume":"15","author":"Depick\u00e8re S","year":"2022","journal-title":"Parasit Vectors"},{"key":"ref33","year":"2003"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"e200528","DOI":"10.1590\/0074-02760200528","article-title":"Systematic review on the biology, ecology, genetic diversity and parasite transmission potential of Panstrongylus geniculatus (Latreille 1811) in Latin America","volume":"116","author":"Vivas RJ","year":"2021","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"5","key":"ref35","first-page":"e170298","article-title":"Acute Chagas disease in the state of Par\u00e1, Amazon Region: is it increasing?","volume":"113","author":"Dos Santos VRC","year":"2018","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003010","article-title":"Acute chagas disease: new global challenges for an old neglected disease.","author":"Andrade 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